Summary
The effect of sodium orthovanadate on enhancement of DNA synthesis by T and B cell mitogenic agents was studied using murine thymocytes and splenocytes. Addition of vanadate to thymocyte cultures inhibited the mitogenic response induced by concanavalin A in a dose dependent manner (50% inhibition at 10 μM). On the other hand, DNA synthesis induced in thymocytes by pokeweed lectin and periodate treatment essentially was not inhibited at the lower vanadate concentrations that were markedly effective for concanavalin A induced synthesis. In addition, no significant inhibition of mitogenesis of splenic B cells in response to lipopolysaccharide and dextran was detectable at lower vanadate concentrations. In the absence of added mitogens, vanadate was found to be mitogenic for a subpopulation of thymus cells but not for splenocytes or T cell enriched splenocyte populations. These results suggest that vanadate affects the mitogenic responses in lymphocytes and that the interaction of vanadate with T and B cells is different.
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References
Underwood, E. J., 1971. In: Trace elements in human and animal nutrition. pp. 416–424. Academic Press, New York.
Post, R. L., Hunt, D. P., Walderhaug, M. O., Perkins, R. C., Park, J. H. and Beth, A. H., 1979. In: 2nd International conference on the properties and functions of Na+-K+-ATPase. pp. 389–401. Academic Press, New York.
Waters, M. D., 1977. Adv. Mod. Toxicol. 2: 147–185.
Werdan, K., Bauriedel, G., Bozsik, M., Krawitz, W. and Erdman, E., 1980. Biochim. Biophys. Acta 597: 364–383.
Shechter, Y. andKarlish, S. J. D., 1980. Nature 284: 556–558.
Dubyak, G. R. and Kleinzeller, A., 1980. J. Biol. Chem. 255: 5306–5312.
Cantley, L. C. Jr., Josephson, L., Warner, R., Yanagisawa, M., Lechene, C. and Guidotti, G., 1977. J. Biol. Chem. 252: 7421–7423.
Cantley, L. C. Jr., Cantley, L. G. and Josephson, L., 1978. J. Biol. Chem. 253: 7361–7368.
O'Neal, S. G., Rhoads, D. B. and Racket, E., 1979. Biochem. Biophys. Res. Commun. 89: 845–850.
Schwabe, U., Puchstein, C., Hannemann, H. and Sochtig, E., 1979. Nature 277: 143–145.
Catalan, R. E., Martinez, A. M. and Aragones, M. D., 1980. Biochem. Biophys. Res. Commun. 96, 672–677.
Koller, L. D., 1980. Int. J. Immunopharmac. 2: 269–279.
Pastan, I. H., Johnson, G. S. and Anderson, W. B., 1975. Ann. Rev. Biochem. 44: 491–522.
Kaplan, J. G., 1977. Regulatory mechanisms in lymphocyte activation (Lucas, D. O., ed.), pp. 51–75, Academic Press, New York.
Swenson, R. M. and Kern, M., 1967. Proc. Natl. Acad. Sci. USA 57: 417–422.
Trizio, D. and Cudkowicz, G., 1974. J. Immunol. 113: 1093–1097.
Zimmerman, D. H. and Kern, M., 1973. J. Immunol. 111: 761–769.
Novogradsky, A., 1974. Eur. J. Immunol. 4: 646–648.
Zimmerman, D. H. and Kern, M., 1973. J. Immunol. 111: 1326–1332.
Gregory, S. H., Zimmerman, D. H. and Kern, M., 1980. J. Immunol. 125: 102–107.
Stobo, J. D. and Paul, W. E., 1973. J. Immunol. 110: 362–375.
Kisielow, P., Hirst, J. A., Shiku, H., Beverly, P. C. L., Hoffmann, M. K., Boyse, E. A. and Oettgen, H. F., 1975. Nature (Lond.) 253: 219–220.
Macara, I. G., Kustin, K. and Cantley, L. C. Jr., 1980. Biochim. Biophys. Acta 629: 95–106.
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Ramanadham, M., Kern, M. Differential effect of vanadate on DNA synthesis induced by mitogens in T and B lymphocytes. Mol Cell Biochem 51, 67–71 (1983). https://doi.org/10.1007/BF00215587
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DOI: https://doi.org/10.1007/BF00215587