Summary
The present study was undertaken to provide information on the presence and frequency of satellite nucleoli in cells with increased nucleolar biosynthetic activity. The number of hepatocytes containing satellite nucleoli was analyzed in rat liver, regenerating liver 18 h after partial hepatectomy and in Novikoff hepatoma ascites cells. In comparison with hepatocytes of normal liver, the number of both stimulated hepatocytes and malignant hepatoma cells containing satellite nucleoli was significantly reduced. The results also indicated that whereas most satellite nucleoli contain protein C23, a smaller percentage contain protein B23.
Similar content being viewed by others
References
Bouteille M, Laval M, Dupuy-Coin AM (1974) Localization of nuclear functions as revealed by ultrastructural autoradiography and cytochemistry. In: Busch H (ed) The cell nucleus. Vol. I. Academic Press, New York, pp 3–71
Bresnick E (1971) Regenerating liver: an experimental model for the study of growth. In: Busch H (ed) Methods in Cancer Research, Vol. VI. Academic Press, New York, pp 347–397
Busch H (1974) Messenger RNA and other high molecular weight RNA. In: Busch H (ed) the Molecular Biology of Cancer. Academic Press, New York, pp 188–276
Busch H, Smetana K (1970) The Nucleolus. Academic Press, New York
Busch H, Daskal Y, Gyorkey F, Smetana K (1979) Silver staining of nucleolar granules in tumor cells. Cancer Res 39:857–863
Busch RK, Daskal Y, Spohn WH, Kellermayer M, Busch H (1974) Rabbit antibodies to nucleoli of Novikoff hepatoma and normal liver of the rat. Cancer Res 34:2362–2367
Daskal Y, Smetana K, Busch H (1980) Evidence from studies on segregated nucleoli that silver stained proteins are in the fibrillar components. Exp Cell Res 127:285–291
Fujioka M, Koga M, Lieberman I (1963) Metabolism of ribonucleic acid after partial hepatectomy. J Biol Chem 238:3401–3406
Goessens G, Lepoint A (1982) Localization of Ag NOR-proteins in Ehrlich tumor cell nucleoli. Biol Cell 43:139–142
Hernandez-Verdun D, Hubert J, Bourgeois CA, Bouteille M (1980) Ultrastructural localization fo Ag-NOR stained proteins in the nucleolus during the cell cylce and in other nucleolar structures. Chromosoma 79:349–362
Hubbel HR, Lau YF, Brown RL, Hsu TC (1980) Cell cylce analysis and drug inhibition studies of silver staining in synchronous HeLa cells. Exp Cell Res 129:139–147
Likovsky Z, Smetana K (1981) Further studies on the cytochemistry of the standardized silver staining of interphase nucleoli in smear preparations of Yoshida ascitic sarcoma cells in rats. Histochemistry 72:301–313
Lischwe MA, Richards RL, Busch RK, Busch H (1981) Localization of phosphoprotein C23 to nucleolar structures and to the nucleolus organizer regions. Exp Cell Res 136:101–109
Miner KM, Reading CL, Nicolson GL (1981) In vivo and in vitro production and detection of monoclonal antibodies to surface components on metastastic variance of murine tumor cells. Invasion metastasis 1:158
Ochs R, Lischwe M, O'Leary P, Busch H (1983) Localization of nucleolar phosphoproteins during mitosis. Exp Cell Res 146:139–149
Ploton D, Bobichon H, Adnet JJ (1982) Ultrastructural localization of NOR in nucleoli of human breast cancer tissues using a one-step Ag NOR staining method. Biol Cell 43:229–232
Schwarzacher HG, Mikelsaar AV, Schnedl W (1978) The nature of the Ag-staining nucleolus organizer regions. Electron and light microscopic studies of human cells in interphase, mitosis and meiosis. Cytogenet Cell Genet 20:24–39
Smetana K, Busch H (1979) Studies on silver stained nucleolar components. In: Busch H, Crooke ST, Daskal Y (eds) Effects of Drugs on the Cell Nucleus. Academic Press, New York, pp 89–105
Smetana GF, Gyorkey P, Busch H (1971) Compact filamentous bodies of nuclei and nucleoli of human prostate gland. Exp Cell Res 64:133–139
Smetana K, Likovsky Z, Busch RK, Busch H (1984) Further studies on satellite nucleoli in rat and mouse hepatocytes. Exp Cell Res 151:80–86
Smetana K, Likovsky Z, Ochs R, Novak J, Busch H (1986) Studies on satellite nucleoli in human normal and leukemic lymphocytes. Virchows Arch [Cell Pathol] 51:155–160
Spector DI, Ochs RL, Busch H (1984) Silver staining, immunofluorescence, and immunoelectron microscopic localization of nucleolar phosphoproteins B23 and C23. Chromosoma (Berl) 90:139–148
Wachtler F, Schwarzacher HG, Smetana K (1984) On the fusion of nucleoli in interphase. Eur J Cell Biol 34:190–192
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Smetana, K., Ochs, R.L., Busch, R.K. et al. Studies on satellite nucleoli in rat hepatocytes and Novikoff hepatoma cells. Cell Tissue Res. 249, 235–239 (1987). https://doi.org/10.1007/BF00215439
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00215439