Summary
Serial sections from kidneys of 5 aglomerular lemon soles (Pleuronectes microcephalus) demonstrated the presence of anastomosing arteriolar networks in the caudal half of the organs. There was no preferred location of the networks, which were found both near the surface and in the deeper parts of the kidney. The size of the networks varied; the largest measured more than 900 μm in the longest axis and covered an area larger than 500000 μm2, whereas the smallest measured about 60 μm in diameter with an area of 2040 μm2. The larger networks were invariably located close to large intrarenal veins. Anastomoses were found between the arteriolar networks and the peritubular capillaries and also associated with veins.
The walls of the arterioles were composed of granulated epithelioid cells, exhibiting numerous intracytoplasmic granules. These granules occasionally had a rod-like appearance with a paracrystalline substructure. Using antibodies directed against murine and human renin, the epithelioid cells in the walls of the arteriolar networks and the cytoplasmic granules revealed a positive immunoreaction.
The results suggest that the renin-angiotensin system in the aglomerular lemon sole may be important for both the regulation of the systemic blood pressure and the blood supply to the peritubular capillaries of the kidney.
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References
Barajas L (1966) The development and ultrastructure of the juxtaglomerular cell granule. J Ultrastruct Res 15:400–413
Bean JW (1942) Specificity in the renin-hypertensinogen reaction. Am J Physiol 136:731–742
Bohle A, Walvig F (1964) Beitrag zur vergleichenden Morphologie der epitheloiden Zellen der Nierenarteriolen unter besonderer Berücksichtigung der epitheloiden Zellen in den Nieren von Seewasserfischen. Klin Wochenschr 9:415–421
Bulger RE, Trump BF (1969) Ultrastructure of granulated arteriolar cells (juxtaglomerular cells) in kidney of a fresh and a salt water teleost. Am J Anat 124:77–87
Capelli JP, Wesson LG, Gonzalo EA (1970) A phylogenetic study of the renin-angiotensin system. Am J Physiol 218:1171–1178
Capréol SV, Sutherland LE (1967) Comparative morphology of juxtaglomerular cells. I. Juxtaglomerular cells in fish. Can J Zool 46:249–256
Churchill PC, Malvin RL, Churchill MC, McDonald F (1979) Renal function in Lophius americanus: effects of angiotensin II. Am J Physiol 236 (5):R297–301
Egerer G, Taugner R, Tiedemann K (1984) Renin immunohistochemistry in the mesonephros and metanephros of the pig embryo. Histochemistry 81:385–390
Lamers APM, Verhofstad AAJ, Stadhouders ADM, Michelakis AM (1985) Immunohistochemical demonstration of renin in the juxtaglomerular apparatus of three Bufo species. Cell Tissue Res 239:677–682
Minuth M, Hackenthal E, Poulsen K, Rix E, Taugner R (1981) Renin immunocytochemistry of the differentiating juxtaglomerular apparatus. Anat Embryol 162:173–181
Mizogami S, Oguri M, Sokabe H, Nishimura H (1968) Presence of renin in the glomerular and aglomerular kidney of marine teleosts. Am J Physiol 215:991–994
Nishimura H, Mizuho O, Sawyer WH (1973) Renin-angiotensin system in primitive bony fishes and a holocephalian. Am J Physiol 224:950–956
Nishimura H, Lunde LG, Zucker A (1979) Renin response to hemorrhage and hypotension in the aglomerular toadfish Opsanus tau. Am J Physiol 237 (2):H105–112
Oguri M, Ogawa M, Sokabe H (1972) Juxtaglomerular cells in aglomerular teleosts. Bull Jp Soc Sci Fish 38:195–200
Rosen S, Tischer CC (1968) Observations on the Rhesus monkey glomerulus and Juxtaglomerular apparatus. Lab Invest 18:240–248
Small JV (1968) Measurements of section thickness. In: Bocciarelli DS (ed) Proceedings 4th European Congress on Electron Microscopy. Tipografia Poliglotta Vaticana. Roma, Vol I, p 609
Taugner Ch, Poulsen K, Hackenthal E, Taugner R (1979) Immunocytochemical localization of renin in mouse kidney. Histochemistry 62:19–27
Taugner R, Hackenthal E, Helmchen U, Ganten D, Kugler P, Marin-Grez M, Nobiling R, Unger Th, Lockwald I, Keilbach R (1982) The intrarenal renin-angiotensinsystem. An immunocytochemical study on the localization of renin, angiotensinogen, converting enzyme and the angiotensins in the kidney of mouse and rat. Klin Wochenschr 60:1218–1222
Taugner R, Bührle CP, Nobiling R, Kirschke H (1985) Coexistence of renin and cathepsin B in epithelioid cell secretory granules. Histochemistry 83:102–108
Taugner R, Yokota S, Bührle CP, Hackenthal E (1986) Catepsin D coexists with renin in the secretory granules of juxtaglomerular epithelioid cells. Histochemistry 84:19–22
Weichert G (1965) Über die Wirkung fraktionierter Nierenextrakte von Vertebraten verschiedener Evolutionsstadien auf den Blutdruck der nephrektomierten Ratte. Pflügers Arch 284:147–159
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Christensen, J.A., Taugner, R., Meyer, D.S. et al. The granular epithelioid cells in the kidney of the lemon sole (Pleuronectes microcephalus Donovani). Cell Tissue Res. 249, 137–143 (1987). https://doi.org/10.1007/BF00215427
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DOI: https://doi.org/10.1007/BF00215427