Summary
Serotonergic cell bodies and fibers were identified in the pedal ganglia of Mytilus gattoprovincialis with a serum raised against serotonin and the unlabelled peroxidase-antiperoxidase pre- and post-embedding methods. Examination of Vibratome and serial semithin sections showed that most reactive perikarya are located in the ganglionic cortex, being mainly concentrated at the medial aspect of the postero-dorsal portion of the ganglia. Immunoreactive fibers form a dense network in the neuropil, extend throughout the commissure and run parallel in the nerves and connective tracts. The morphology of serotonin-positive cells compared with that of Golgi-impregnated neurons allows the identification of a main population of unipolar, probably projecting neurons and of smaller multipolar cells likely representing local circuit elements. The ultrastructure of labelled neurons is comparable to that of serotonergic cells described in both vertebrate and invertebrate nervous systems.
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References
Boer HH, Schot LPC, Steinbusch HWM, Montagne C, Reichelt D (1984) Co-existence of immunoreactivity to anti-dopamine, anti-serotonin and anti-vasotocin in the cerebral giant neuron of the pond snail Lymnaea stagnalis. Cell Tissue Res 238:411–412
Bullock TH, Horridge GA (1965) Structure and function in the nervous system of invertebrates. Vol I, II, WH Freeman and Company San Francisco and London
Dahl E, Falk B, Von Mecklenburg C, Myhrburg H, Rosengren E (1966) Neuronal localization of dopamine and 5-hydroxy-tryptamine in some mollusca. Z Zellforsch 71:489–498
De Biasi S, Vitellaro-Zuccarello L, Blum I (1984) Histochemical localization of monoamines and cholinesterases in Mytilus pedal ganglion. Histochemistry 81:561–565
Gerschenfeld HM (1973) Chemical transmission in invertebrate central nervous systems and neuromuscular junctions. Physiol Rev 53:1–119
Kapadia SE, De Lanerolle NC, La Motte CC (1985) Immunocytochemical and electron microscopic study of serotonin neuronal organization in the dorsal raphe nucleus of the monkey. Neuroscience 15:729–746
Matsutani T, Nomura T (1986) Serotonin-like immunoreactivity in the central nervous system and gonad of the scallop Patinopecten yessoensis. Cell Tissue Res 244:515–517
Maxwell MH (1978) Two rapid and simple methods used for the removal of resins from 1.0 μ thick epoxy sections. J Microsc 112:253–255
Nässel DR, Meyer EP, Klemm N (1985) Mapping and ultrastructure of serotonin-immunoreactive neurons in the optic lobes of three insect species. J Comp Neurol 232:190–204
Ono JK, McCaman RE (1984) Immunocytochemical localization and direct assays of serotonin-containing neurons in Aplysia. Neuroscience 11:549–560
Osborne NN, Cuello AC, Dockray GJ (1982) Substance P and cholecystokinin-like peptides in Helix neurons and cholecystokinin and serotonin in a giant neuron. Science 216:409–411
Patterson JA (1985) A modified, prefixed, Golgi-rapid technique for the cephalopod retina. J Neurosci Methods 12:219–225
Pentreath VW, Berry MS (1978) Radioautographic study of 5-hydroxytryptamine-containing nerve terminals in central ganglia of Planorbis corneus: comparison with other species and characteristics of the serotoninergic nerve terminal. J Neurocytol 7:443–459
Roubos EW, Moorer-Van Delft CM (1979) Synaptology of the central nervous system of the freshwater snail Lymnaea stagnalis (L.), with particular reference to neurosecretion. Cell Tissue Res 198:217–235
S-Rozsa K (1984) The pharmacology of molluscan neurons. Progr Neurobiol 23:79–150
Stefano GB, Aiello E (1975) Histofluorescent localization of serotonin and dopamine in the nervous system and gill of Mytilus edulis (Bivalvia). Biol Bull 148:141–156
Stefano GB, Martin R (1983) Enkephalin-like immunoreactivity in the pedal ganglion of Mytilus edulis (Bivalvia) and its proximity to dopamine-containing structures. Cell Tissue Res 230:147–153
Stefano GB, Catapane EJ, Aiello E (1976) Dopaminergic agents: influence on serotonin in the molluscan nervous system. Science 194:539–541
Sternberger LA (1979) Immunocytochemistry. Second edition, John Wiley and Sons, New York Chichester
Sweeney D (1968) The anatomical distribution of monoamines in freshwater bivalve mollusc Sphaerium sulcantiem. Comp Biochem Physiol 25:601–614
Tyrer NM, Turner JD, Altman JS (1984) Identifiable neurons in the locust central nervous system that react with antibodies to serotonin. J Comp Neurol 227:313–330
Vitellaro-Zuccarello L, De Biasi S, Blum I (1983) Histochemical and ultrastructural study on the innervation of the byssus glands of Mytilus galloprovincialis. Cell Tissue Res 233:403–413
Walker RJ (1985) 5-hydroxytryptamine in invertebrates. Comp Biochem Physiol 79C:231–235
Welsh JH, Moorehead M (1960) The quantitative distribution of serotonin in invertebrates, especially in their nervous system. J Neurochem 6:146–169
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De Biasi, S., Vitellaro-Zuccarello, L. Distribution of 5HT-immunoreactivity in the pedal ganglion of Mytilus galloprovincialis . Cell Tissue Res. 249, 111–116 (1987). https://doi.org/10.1007/BF00215424
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DOI: https://doi.org/10.1007/BF00215424