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Temperature dependence of the fluorescence of pyrene labeled crab nerve membranes

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Summary

A method, using albumin-pyrene complexes, has been developed for labeling, in a controlled manner, crab leg nerves whose excitability was preserved.

The excimer-to-monomer fluorescence intensity ratio of pyrene, embedded in nerve membrane lipids and in their crude lipid extracts, is a fluidity parameter which displayed the following features with temperatures.

a—a temperature-dependent increase of fluidity

b—three breaks (6°, 19° and 37°C) in the physiological medium

c—In Ca ++-depleted sea water, the 37° characteristic temperature vanished.

These breaks may reflect some lateral phase separations of the lipid components of nerve membranes. The calcium dependent temperature break may involve a segregation of acidic phospholipids while the other two breaks (6° and 19°C) may be due to neutral lipids phase separation. The relationship of these findings to nerve function is discussed.

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References

  1. Lenaz, G., Curatola, G., Mazzanti, L. and Parenti-Castelli, G., 1978. Mol. Cell. Biochem. 22, 3–32.

    Google Scholar 

  2. Kimelberg, H. K., 1977. In: Dynamic aspects of cell surface organization (Poste, G. and Nicolson, G. L. eds) pp. 205–293, North-Holland, Amsterdam.

  3. Lee, A. G., 1976. Nature 262, 545–548.

    Google Scholar 

  4. Keynes, R. D., 1972. Nature 239, 29–32.

    Google Scholar 

  5. Hille, B., 1978. Biophys. J. 22, 283–294.

    Google Scholar 

  6. Hubbell, W. L. and McConnell, H. M., 1969. Proc. Natl. Acad. Sci. USA 64, 20–27.

    Google Scholar 

  7. Hubbell, W. L. and McConnell, H. M., 1971. J. Amer. Chem. Soc. 93, 314–326.

    Google Scholar 

  8. Birks, J. B., 1970. Photophysics of Aromatic Molecules, Wiley Interscience, New York.

    Google Scholar 

  9. Vanderkooi, J. M. and Callis, J. B., 1974. Biochemistry 13, 4000–4006.

    CAS  PubMed  Google Scholar 

  10. Morrisett, J. D., Pownall, H. J., Plumlee, R. T., Smith, L. C., Zehner, Z. E., Esfahani, M. and Wakil, S. J., 1975. J. Biol. Chem. 250, 6969–6976.

    Google Scholar 

  11. Dembo, M., Glushko, V., Aberlin, M. E. and Sonenberg, M., 1979. Biochim. Biophys. Acta 522, 201–211.

    Google Scholar 

  12. Galla, H. J. and Sackman, E., 1974. Biochim. Biophys. Acta 339, 103–115.

    Google Scholar 

  13. Bothorel, P. and Desmazès, J. P., 1974. Biochim. Biophys. Acta 365, 181–192.

    Google Scholar 

  14. Bothorel, P., Desmazès, J. P. and Georgescauld, D., 1977. C.R. Acad. Sci. Paris 289, 491–493.

    Google Scholar 

  15. Lowry, O. H., Rosebrough, M. J., Farr, A. L. and Randall, R. J., 1951. J. Biol. Chem. 193, 265.

    CAS  PubMed  Google Scholar 

  16. Furusawa, I., 1929. J. Physiol. (London) 67, 325–342.

    Google Scholar 

  17. Trauble, H. and Sackmann, E., 1972. J. Amer. Chem. Soc. 94, 4499–4510.

    Google Scholar 

  18. Courchaine, A. J., Miller, W. H. and Stein, Jr. D. B., 1939. Clin. Chem. 5, 609.

    Google Scholar 

  19. Allen. R. J. L., 1940. Biochem. J. 34, 858–865.

    Google Scholar 

  20. Balerna, M., Fosset, M. Chicheportiche, R., Romey, G. and Lazdunski, M., 1975. Biochemistry 14, 5500–5511.

    Google Scholar 

  21. De Bernard, L., 1958. Bull. Soc. Chem. Biol. 40, 161–170.

    Google Scholar 

  22. Bligh, E. G. and Dyer W. J., 1959. Canad. J. Biochem. Physiol. 37, 911–917.

    Google Scholar 

  23. Desmazès, J. P., 1973. Thèse Docteur-Ingénieur, Université de Bordeaux I.

  24. Cohen, L. B., Salzberg, B. M., Davila, H. V., Ross, W. N., Landowne, D., Waggoner, A. S. and Chang, C. H., 1974. J. Membrane Biol. 19, 1–36.

    Google Scholar 

  25. Podo, F. and Blasie, J. K., 1977. Proc. Natl. Acad. Sci. USA 74, 1032–1036.

    Google Scholar 

  26. Vanderkooi, J. M., Fischkoff, S., Andrich, M., Podo, F. and Owen, C. S., 1975. J. Chem. Phys. 63, 3661–3666.

    Google Scholar 

  27. Von Muralt, A., Weibel, E. R. and Howarth, J. V., 1976. Pflugers Arch. 367, 67–76.

    Google Scholar 

  28. Georgescauld, D., Rome-Talbot, D. and Chalazonitis, N., 1974. C.R. Acad. Sci. Paris 279, 939–943.

    Google Scholar 

  29. Ito, T. and Ohnishi, S. I., 1974. Biochim. Biophys. Acta 352, 30.

    Google Scholar 

  30. Blazyk, J. F. and Steim, M., 1972. Biochim. Biophys. Acta 266, 737–741.

    Google Scholar 

  31. Hodgkin, A. L. and Huxley, A. F., 1952. J. Physiol. (London) 117, 500–544.

    Google Scholar 

  32. Gache, C., Rossi, B. and Lazdunski, M., 1976. Eur. J. Biochem. 65, 293–306.

    Google Scholar 

  33. Tasaki, I., 1968. Nerve excitation. A macromolecular approach. Charles C. Thomas, Springfield.

    Google Scholar 

  34. Georgescauld, D. and Duclohier, H., 1978. Biochem. Biophys. Res. Commun. 85, 1186–1191.

    Google Scholar 

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Authors and Affiliations

  1. Centre de Recherches Paul Pascal, Domaine Universitaire, 33405, Talence Cedex, France

    D. Georgescauld, J. P. Desmazes & H. Duclohier

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  1. D. Georgescauld
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  2. J. P. Desmazes
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  3. H. Duclohier
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Georgescauld, D., Desmazes, J.P. & Duclohier, H. Temperature dependence of the fluorescence of pyrene labeled crab nerve membranes. Mol Cell Biochem 27, 147–153 (1979). https://doi.org/10.1007/BF00215363

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  • DOI: https://doi.org/10.1007/BF00215363

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