Abstract
The vomeronasal organs (VNOs) of rats were cultured from embryonic 15-day littermates on collagen-coated membrane in Dulbecco's modified Eagle's medium containing serum. The explants were observed sequentially and fixed at 4, 6, 8, 10 and 14 days in vitro (DIV). Organogenesis of VNOs and cell differentiation took place in vitro. Patterns of organogenesis of the VNO in vitro were different from those in vivo. Both sensory and supporting cells in the sensory epithelium had microvilli on their surface. Epithelial cells in aggregates of non-sensory epithelial cells had cilia and microvilli on their surface. Vomeronasal axons forming two to three large fascicles were seen originating from the VNO at 4, 6, and 8 DIV, and degenerated at 10 or 14 DIV. Glial cells (ensheathing cells) were observed in the fascicles. These morphological characteristics of VNO cells in vitro were similar to those observed in vivo.
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References
Bolz J, Novak N, Gotz M, Bonhoeffer T (1990) Formation of target-specific neuronal projections in organotypic slice cultures from rat visual cortex. Nature 346:359–362
Bolz J, Gotz M, Hubener M, Novak N (1993) Reconstructing cortical connections in a dish. Trends Neurosci 16:310–316
Chuah MI, Au C (1991) Olfactory Schwann cells are derived from precursor cells in the olfactory epithelium. J Neurosci Res 29:172–180
Doucette R (1984) The glial cells in the nerve fiber layer of the rat olfactory bulb. Anat Rec 210:385–391
Doucette R (1989) Development of the nerve fiber layer in the olfactory bulb of mouse embryo. J Comp Neurol 285:514–527
Farbman AI (1977) Differentiation of olfactory receptor cells in organ culture. Anat Rec 189:187–200
Garrosa M, Coca S (1991) Postnatal development of the vomeronasal epithelium in the rat: an ultrastructural study. J Morphol 208:257–269
Garrosa M, Coca S, Mora OA (1986) Histological development of the vomeronasal complex in the pre- and postnatal rat. Acta Otolaryngol (Stockh) 102:291–301
Garrosa M, Iniguez C, Fernandez M, Gayoso MJ (1992) Developmental stages of the vomeronasal organ in the rat: a light and electron microscopic study. J Hirnforsch 33:123–132
Gonzales F, Farbman AI, Gesteland RC (1985) Cell and explant culture of olfactory chemoreceptor cells. J Neurosci Methods 14:77–90
Halatz N (1990) The vertebrate olfactory system. Acadenia Kiado, Budapest
Halpern M (1987) The organization and function of the vomeronasal system. Ann Rev Neurosci 10:325–362
Ichikawa M, Matsuoka M, Mori Y (1993a) Effect of differential rearing on synapses and soma size in rat medial amygdaloid nucleus. Synapse 13:50–56
Ichikawa M, Matsuoka M, Mori Y (1993b) Effect of differential rearing on perforated synapses in the granule cell layer of rat's accessory olfactory bulb. Neurosci Res 18:19–25
Matsuoka M, Mori Y, Ichikawa M (1994) Social environment affects synaptic stucture in the glomerulus of hamster's accessory olfactory bulb. Neurosci Res 19:187–193
Raisman G (1985) Specialized neurological arrangement may explain the capacity of vomeronasal axons to reinnervate central neurons. Neuroscience 14:237–254
Valverde F, Santacana, Heredia M (1992) Formation of an olfactory glomerulus: Morphological aspects of development and organization. Neuroscience 49:255–275
Wysocki CJ (1979) Neurobehavioral evidence for the involvement of the vomeronasal system in mammalian reproduction. Neurosci Behav Rev 3:301–340
Yamamoto N, Kurotani T, Toyama K (1989) Neuronal connections between the lateral geniculate nucleus and visual cortex in vitro. Science 245:192–195
Yamamoto N, Yamada K, Kurotani T, Toyama K (1992) Laminar specificity of extrinsic cortical connections studied in coculture preparations. Neuron 9:217–228
Yoshida J, Kimura J, Tsukise A, Okano M (1993) Developmental study on the vomeronasal organ in the rat fetus. J Reprod Dev 39:47–54
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Ichikawa, M., Osada, T. Morphology of vomeronasal organ cultures from fetal rat. Anat Embryol 191, 25–32 (1995). https://doi.org/10.1007/BF00215294
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DOI: https://doi.org/10.1007/BF00215294