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Recognition by peptide mapping of three different structural groups of outer membrane protein YOP-1 of Yersinia enterocolitica and Yersinia pseudotuberculosis

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Abstract

The structural relation of YOP-1 of “european” and “american” Yersinia enterocolitica serotypes O∶3, O∶9, O∶5, 27, and O∶8 and O∶20, respectively, and Y. pseudotuberculosis serotypes I, II, and III was compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis and peptide mapping using Staphylococcus aureus protease V8. Apparent molecular weights of YOP-1 ranged from 206,000 (O∶3) to approx. 180,000 (O∶8). According to their respective peptide maps YOP-1 of the “european” and “american” Y. enterocolitica serotypes and Y. pseudotuberculosis serotypes could be assigned to three different groups. Evaluation of several isolates of Y. enterocolitica serotypes O∶3, O∶9, and O∶8 by peptide mapping indicated that YOP-1 is conserved within a serotype. However, one serotype O∶8 isolate differed from the consensus peptide pattern of the other serotype O∶8 and O∶20 isolates. The similarity of the peptide patterns of Yersinia serotypes which predominate in certain geographical locations, i. e., “european” and “american” Y. enterocolitica serotypes, suggest common evolution of YOP-1 of these serotypes independent of the evolution of the other serotypes.

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  1. Jürgen Heesemann

    Present address: Institut für Hygiene und Mikrobiologie, Universität Würzburg, Josef-Schnei-der-Str. 2, 1, Würzburg, FRG

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  1. Institute for Medical Microbiology and Immunology, University Hospital Eppendorf, Martinistrasse 52, 20, Hamburg, Federal Republic of Germany

    Dietrich Mack, Mathias Pulz & Jürgen Heesemann

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  1. Dietrich Mack
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Mack, D., Pulz, M. & Heesemann, J. Recognition by peptide mapping of three different structural groups of outer membrane protein YOP-1 of Yersinia enterocolitica and Yersinia pseudotuberculosis . Med Microbiol Immunol 180, 205–211 (1991). https://doi.org/10.1007/BF00215249

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  • DOI: https://doi.org/10.1007/BF00215249

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