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Catecholamines in the coelenterate Renilla köllikeri

Uptake and radioautographic localization

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Summary

The characteristics of uptake of 3H-noradrenaline (3H-NA) and 3H-adrenaline (3H-A) in the tissues of the sea pansy, Renilla köllikeri, were studied by in vivo incubations. Lineweaver-Burk plots indicated two components of catecholamine accumulation, one representing a high-affinity uptake with an apparent K m of 4.91×10-7 M (3H-NA) or 4.39×10-7 M (3H-A), and the other a low affinity process with an apparent Km of 5.52×10-5 M (3H-NA) or 1.49×10-5 M (3H-A). The high-affinity uptake of both tracers was strongly inhibited at low temperature and in a calcium-free medium, thus suggesting the involvement of a carrier-mediated transport mechanism, but was largely insensitive to sodium omission and ouabain. Accumulations of 3H-NA, but not 3H-A, were highly desipramine-sensitive.

Light-microscopic radioautographic studies demonstrated the presence of cells reactive to both 3H-NA and 3H-A in the ectoderm, mesoglea and endoderm. Extraneuronal accumulations of 3H-NA and 3H-A were prominent in some ectodermal cells, in amoebocytes and spicule cells. Reactive neuronal processes were tentatively identified throughout the mesoglea and over all muscle layers on the basis of several morphological criteria. 3H-A, but not 3H-NA label, was more intense over the presumed photocytic zone and circular muscle than elsewhere. These and other observations support a neurotransmitter role for adrenaline (and probably noradrenaline) in control of luminescence and modulation of slow rachidial contractions.

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Authors and Affiliations

  1. Département de Sciences biologiques, Université de Montréal, Montréal, Canada

    Michel Anctil, Guy Germain & Luc LaRivière

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  1. Michel Anctil
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  2. Guy Germain
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  3. Luc LaRivière
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Supported by a grant from the Natural Sciences and Engineering Research Council of Canada

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Anctil, M., Germain, G. & LaRivière, L. Catecholamines in the coelenterate Renilla köllikeri . Cell Tissue Res. 238, 69–80 (1984). https://doi.org/10.1007/BF00215146

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