Abstract
The mitochondria of chive plants with normal N or male-sterile S cytoplasms have been examined by restriction fragment analysis and Southern hybridizations of mitochondrial DNA (mtDNA) and in organello protein biosynthesis. Restriction fragment patterns of the mtDNA differed extensively between N-and S-cytoplasms. The percentage of fragments with different mobility varied between 44–48% depending on the restriction enzyme used. In contrast to mtDNA, the restriction fragment patterns of the chloropolast DNA from N- and S-cytoplasms were identical. The organization of the analyzed mitochondrial genes coxII, coxIII, nad1 and nad3 was different in N- and S-cytoplasms. Comparison of mitochondrial proteins analyzed by in organello translation revealed an 18-kDa protein present only in S-cytoplasm. The restorer gene X suppressed the synthesis of that protein in S-cytoplasm. Thus, the 18-kDa protein seems to be associated with the cytoplasmic male-sterile phenotype.
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Bailey-Serres J, Dixon L, Liddell AD, Leaver CJ (1986a) Nuclear-mitochondrial interactions in cytoplasmic male sterile Sorghum. Theor Appl Genet 73:252–260
Bailey-Serres J, Hanson DK, Fox TD, Leaver CJ (1986b) Mitochondrial genome rearrangement leads to extention and relocation of the cytochrome c oxidase subunit I gene in Sorghum. Cell 47:567–576
Boutry M, Briquet M (1982) Mitochondrial modifications associated with the cytoplasmic male sterility in faba beans. Eur J Biochem 127:129–135
Boutry M, Faber AM, Charbonnier M, Briquet M (1984) Microanalysis of plant mitochondrial protein synthesis products. Plant Mol Biol 3:445–452
Brears T, Schardl CL, Lonsdale DM (1986) Chloroplast genome organisation in sugar beet and maize. Plant Mol Biol 6:171–177
Chase CD, Pring DR (1986) Properties of the linear N1 and N2 plasmid-like DNAs from mitochondria of cytoplasmic malesterile Sorghum bicolor. Plant Mol Biol 6:53–64
Church GM, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81:1191–1995
Crouzillat D, Leroy P, Perrault A, Ledoigt G (1987) Molecular analysis of the mitochondrial genome of Helianthus annuus in relation to cytoplasmic male sterility and phylogeny. Theor Appl Genet 74:773–780
Dewey RE, Timothy DH, Levings CS, III (1987) A mitochondrial protein associated with cytoplasmic male sterility in the T cytoplasm of maize. Proc Natl Acad Sci USA 84:5374–5378
De Courcel AGL, Vedel F, Boussac JM (1989) DNA polymorphism in Allium cepa cytoplasms and its implications concerning the origin of onions. Theor Appl Genet 77:793–798
Dixon LK, Leaver CJ, Brettell RIS, Gengenbach BG (1982) Mitochondrial sensitivity to Drechslera maydis T-toxin and the synthesis of a variant mitochondrial polypeptide in plants derived from maize tissue cultures with texas male-sterile cytoplasm. Theor Appl Genet 63:75–80
Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Ann Biochem 132:6–13
Feinberg AP, Vogelstein B (1984) Addendum: a technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Ann Biochem 137:266–267
Forde BG, Leaver CJ (1980) Nuclear and cytoplasmic genes controlling synthesis of variant mitochondrial polypeptides in male-sterile maize. Proc Natl Acad Sci USA 77:418–422
Fu HY (1983) Variation in mitochondrial translation products and cytoplasmic male sterility in Sorghum. Acta Genet Sinica 10:471–476
Holford P, Croft JH, Newbury HJ (1991) Differences between, and possible origins of, the cytoplasms found in fertile and male-sterile onions (Allium cepa L.). Theor Appl Genet 82:737–744
Horn R, Köhler RH, Zetsche K (1991) A mitochondrial 16 kDa protein is associated with cytoplasmic male sterility in sunflower. Plant Mol Biol 17:29–36
Kobabe G (1965) Möglichkeiten zur züchterischen Verbesserung von Schnittlauchsorten. Gemüse 1:171–172
Köhler RH, Horn R, Lössl A, Zetsche K (1991) Cytoplasmic male sterility in sunflower is correlated with the co-transcription of a new open reading frame with the atpA gene. Mol Gen Genet 227:369–376
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Leaver CJ (1989) Structure and function of the mitochondrial genome in relation to cytoplasmic male sterility. Vortr Pflanzenzuecht 16:379–390
Leaver CJ, Hack E, Forde BG (1983) Protein synthesis by isolated plant mitochondria. Methods Enzymol 97:476–484
Lee SH, Muthukrishnan SM, Sorensen EL, Liang GH (1989) Restriction endonuclease analysis of mitochondrial DNA from Sorghum with fertile and male sterile cytoplasms. Theor Appl Genet 77:379–382
Nivison HT, Hanson MR (1989) Identification of a mitochondrial protein associated with cytoplasmic male sterility in Petunia. Plant Cell 1:1121–1130
Pingitore M, Matthews B, Bottino PJ (1989) Analysis of the mitochondrial genome of Daucus carota with male sterile and male fertile cytoplasms. J Hered 80:143–145
Potz H (1992) Interaktion zwischen Chondriom und Kerngenom im CMS-System bei Schnittlauch (Allium schoenoprasum L.) und Variabilität des Chondrioms unterschiedlicher Provenienzen. PhD thesis, Fachbereich Gartenbau, University of Hanover, Germany
Ruge B, Potz H, Tatlioglu T (1993) Influence of different cytoplasms and nuclear genes involved in the CMS-system of chives (Allium schoenoprasum L.) on microsporogenesis. Plant Breed 110:24–28
Scheike R, Gerold E, Brennicke A, Mehring-Lemper M, Wricke G (1992) Unique patterns of mitochondrial genes, transcripts and proteins in different male-sterile cytoplasms of Daucus carota. Theor Appl Genet 83:419–427
Siculella L, Palmer JD (1988) Physical and gene organization of mitochondrial DNA in fertile and male sterile sunflower. CMS-associated alterations in structure and transcription of the atpA gene. Nucleic Acids Res 16:3787–3799
Singh VP, Kobabe G (1969) Cyto-morphological investigation on male sterility in A. schoenoprasum L. Indian J Genet 29:241–247
Tatlioglu T (1982) Cytoplasmic male sterility in chives (Allium schoenoprasum L.). Z Pflanzenzuecht 89:251–262
Tatlioglu T (1985) Influence of temperature on the expression of cytoplasmic male sterility in chives (Allium schoenoprasum L.). Z Pflanzenzuecht 94:156–161
Tatlioglu T (1986) Influence of tetracycline on the expression of cytoplasmic male sterility (cms) in chives (Allium schoenoprasum L.). Plant Breed 97:46–55
Tatlioglu T (1987) Genetic control of temperature-sensitivity of cytoplasmic male sterility (cms) in chives (Allium schoenoprasum L.) Plant Breed 99:65–76
Tatlioglu T, Wricke G (1988) Genetic control of tetracycline sensitivity of cytoplasmic male sterility (cms) in chives (Allium schoenoprasum L.). Plant Breed 100:34–40
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Communicated by D. R. Pring
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Potz, H., Tatlioglu, T. Molecular analysis of cytoplasmic male sterility in chives (Allium schoenoprasum L.). Theoret. Appl. Genetics 87, 439–445 (1993). https://doi.org/10.1007/BF00215089
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DOI: https://doi.org/10.1007/BF00215089