Summary
Immunohistochemistry and fluorescence histochemistry were applied to the skin of the frog, Bombina orientalis, to detect the localization of bombesin and 5-hydroxytryptamine. The dermal layer contains three types (type 1, 2 and 3) of cutaneous glands but only the type-1 gland simultaneously contains bombesin and 5-hydroxytryptamine. This result suggests for the first time the coexistence of a bioactive peptide and monoamine in a cutaneous gland of the frog.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anastasi A, Erspamer V, Cei JM (1964) Isolation and amino acid sequence of physalaemin, the main active polypeptide of the skin of Physalaemus fuscumaculatus. Arch Biochem Biophys 108:341–348
Chan-Palay V, Jonsson G, Palay SL (1978) Serotonin and substance P coexist in neurons of the rat's central nervous system. Proc Natl Acad Sci USA 75:1582–1586
Coons AH, Leduc EH, Connolly JM (1955) Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J Exp Med 102:49–63
Erspamer V, Bertaccini G, Cei JM (1962) Occurrence of an eledoisin-like polypeptide (physalaemin) in skin extracts of Physalaemus fuscumaculatus. Experientia 18:562–563
Erspamer V, Bertaccini G, Urakawa N (1964) Biogenic amines and active polypeptides in the skin of ten Japanese amphibian species. Jpn J Pharmacol 14:468–473
Falck B, Hillarp N-A, Thieme G, Trop A (1962) Fluorescence of catecholamines and related compounds condensed with formaldehyde. J Histochem Cytochem 10:348–354
Fujita T (1983) Messenger substances of neurons and paraneurons: Their chemical nature and the routes and ranges of their transport to targets. Biomed Res 4:239–256
Hökfelt T, Johansson O, Ljungdahl Å, Lundberg JM, Schultzberg M (1980) Peptidergic neurones. Nature 284:515–521
Kobayashi S, Uchida T (1983) Adrenal chromaffin cells: Immunocytochemical demonstration of catecholamines and opioid peptides. Gunma Symp Endocrinol 20:39–49
Kobayashi S, Uchida T, Ohashi T, Fujita T, Imura H, Mochizuki T, Yanaihara C, Yanaihara N (1983a) Met-enkephalin-Arg-Gly-Leu-like immunoreactivity in adrenal chromaffin cells and carotid body chief cells of the dog and monkey. Biomed Res 4:201–210
Kobayashi S, Ohashi T, Fujita T, Nakao K, Yoshimasa T, Imura H, Mochizuki T, Yanaihara C, Yanaihara N, Verhofstad AAJ (1983a) An immunohistochemical study on the co-storage of Met-enkephalin-Arg6-Gly7-Leu8 and Met-enkephalin-Arg6-Phe7 with adrenaline and/or noradrenaline in the adrenal chromaffin cells of the rat, dog, and cat. Biomed Res 4:433–442
Kobayashi S, Uchida T, Ohashi T, Fujita T, Nakao K, Yoshimasa T, Imura H, Mochizuki T, Yanaihara C, Yanaihara N, Verhofstad AAJ (1983c) Immunocytochemical demonstration of the co-storage of noradrenaline with Met-enkephalin-Arg6-Phe7 and Met-Enkephalin-Arg6-Gly7-Leu8 in the carotid body chief cells of the dog. Arch Histol Jpn 46:713–722
Kobayashi S, Ohashi T, Uchida T, Nakao K, Imura H, Yanaihara N, Verhofstad AAJ (1984) Co-storage of adrenaline and noradrenaline with Met-enkephalin-Arg6-Gly7-Leu8 and Met-enkephalin-Arg6-Phe7 in chromaffin cells of hamster adrenal medulla. Arch Histol Jpn 47:319–336
Legay C, Saffrey MJ, Burnstock G (1984) Coexistence of immunoreactive substance P and serotonin in neurones of the gut. Brain Res 302:379–382
Nakajima T (1980) Evolutional aspects of hormone-like peptides. In: Ishii S et al. (eds) Hormones, adaptation and evolution. Japan Sci Soc Press, Tokyo/Springer-Verlag, Berlin pp 287–293
Nakajima T (1981) Active peptides in amphibian skin. Trends Pharmacol Sci 2:202–205
Nihei K, Iwanaga T, Yanaihara N, Mochizuki T, Fujita T (1983) Preproenkephalin A occurs in the enterochromaffin (EC) cells of the porcine intestine: An immunocytochemical study using antisera to Met-enkephalin-Arg6-Gly7-Leu8 and to serotonin. Biomed Res 4:393–398
Nishiitsutsuji-Uwo J, Takeda M, Saito H (1984) The production of an antiserum to serotonin and serotonin-like immunoreactivity in the cockroach brain-midgut system. Biomed Res 5:211–224
Pelletier G, Steinbusch HWM, Verhofstad AAJ (1981) Immunoreactive substance P and serotonin present in the same dense-core vesicles. Nature 293:71–72
Polak JM, Pearse AGE (1970) Anti-gastrin immunofluorescence in the skin of Hyla crepitons and the cytochemistry of the cells involved. Experientia 26:288–289
Ravazzola M, Brown D, Leppaluoto J, Orci L (1979) Localisation by immunofluorescence of thyrotropin-releasing hormone in the cutaneous glands of the frog, Rana ridibunda. Life Sci 25:1331–1334
Takahashi S, Yui R (1983) Gastrin-releasing peptide (GRP) and serotonin in the human fetal lung: An immunohistochemical study. Biomed Res 4:315–320
Yanaihara C, Inoue A, Mochizuki T, Ozaki J, Sato H, Yanaihara N (1980) Bombesin-like immunoreactivity in mammalian tissues. Biomed Res 1:96–100
Yanaihara N, Yanaihara C, Mochizuki T, Iwahara K, Fujita T, Iwanaga T (1981) Immunoreactive GRP. Peptides 2, Suppl 2:185–191
Yasuhara T, Nakajima T (1975) Occurrence of Pyr-His-Pro-NH2 in the frog skin. Chem Pharm Bull 23:3301–3303
Yasuhara T, Hira M, Nakajima T, Yanaihara N, Yanaihara C, Hashimoto T, Sakura N, Tachibana S, Araki K, Bessho M, Yamanaka T (1973) Active peptides on smooth muscle in the skin of Bombina Orientalin Boulenger and characterization of a new bradykinin analogue. Chem Pharm Bull 21:1388–1391
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yoshie, S., Iwanaga, T. & Fujita, T. Coexistence of bombesin and 5-hydroxytryptamine in the cutaneous gland of the frog, Bombina orientalis . Cell Tissue Res. 239, 25–29 (1985). https://doi.org/10.1007/BF00214898
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00214898