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Clostridium botulinum type C toxin

A sketch of the molecule

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Summary

The purification and crystallization of type C botulinum toxin along with its physical characteristics are described. The shape of Clostridium botulinum type C toxin molecule is globular like a pressed ball with a 7.4 nm diameter and a 4.3 urn thickness. The molecular volume is approximately 185 nl and the molecular weight is 141 000. The toxin molecule is composed of two parts, which are separable under appropriate conditions. These parts have some differences in the electrophoretic properties, amino acid distribution, immunological, and functional characteristics.

The toxin molecule can be reconstituted by association of S-S bond between the two chains. The expression of the toxicity requires that the fragments of the polypeptide chain carrying the necessary information be functionally organized for the proper development of the specific tertiary structure for active conformation.

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Authors and Affiliations

  1. Dept. of Biochemistry, Faculty of Veterinary Medicine, Hokkaido University, 060, Sapporo, Japan

    Bunei Syuto & Shuichiro Kubo

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  1. Bunei Syuto
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  2. Shuichiro Kubo
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Syuto, B., Kubo, S. Clostridium botulinum type C toxin. Mol Cell Biochem 48, 25–32 (1991). https://doi.org/10.1007/BF00214819

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  • DOI: https://doi.org/10.1007/BF00214819

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