Summary
Insulin binding sites are present on purified nuclear envelopes from liver and other tissues, and EM autoradiographs and other types of studies indicate that insulin can enter intact target cells and interact with several types of intracellular membranes, including the nuclear envelope. More recent studies indicate that insulin has direct effects on both mRNA efflux from isolated nuclei and nuclear envelope NTPase, the enzyme that regulates mRNA efflux. These studies raise the possibility, therefore, that insulin regulates mRNA levels in target cells by directly influencing nuclear membrane functions as NTPase. Since insulin does not dramatically elevate mRNA levels for all proteins, the question arises as to how insulin selectively increases mRNA for specific mRNAs. One possibility is that there is targeting of specific mRNA molecules for specific pore complexes and that insulin may only influence a certain fraction of the nuclear pores. Thus, continued investigation is needed concerning the role of polypeptide hormones such as insulin in nucleocytoplasmic exchange.
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Goldfine, I. D., 1978. Life Sci. 23: 2639–2648.
Goldfine, I. D., 1981. Biochemical Actions of Hormones (Litwack, G., ed.) Vol. 8, pp. 273–305, Academic Press, New York.
Horvat, A., Li, E. & Katsoyannis, P. G., 1975. Biochim. Biophys. Acta 382: 609–620.
Goldfine, I. D. & Smith, G. J., 1976. Proc. Natl. Acad. Sci. USA 73: 1427–1431.
Goidl, J. A., 1979. Biochemistry 18: 3674–3679.
Vigneri, R., Pliam, N. B., Cohen, D. C., Pezzino, V., Wong, K. Y. & Goldfine, I. D., 1978. J. Biol. Chem. 253: 8192–8197.
Brisson-Lougarre, A. & Blum, C. J., 1979. C.R. Acad. Sci. (D) (Paris) 289: 129–132.
Brisson-Lougarre, A. & Blum, C. J., 1980. C.R. Acad. Sci. (D) (Paris) 290: 889–892.
Vigneri, R., Goldfine, I. D., Wong, K. Y., Smith, G. J. & Pezzino, V., 1978. J. Biol. Chem. 253: 2098–2103.
Horvat, A., 1978. J. Cell Physiol. 97: 37–48.
Goldfine, I. D., Vigneri, R., Cohen, D. & Pliam, N. B., 1977. Nature 269: 698–700.
Schumm, D. E. & Webb, T. E., 1978. J. Biol. Chem. 253: 8513–8517.
Purrello, F., Vigneri, R., Clawson, G. A. & Goldfine, I. D. 1982. Science 216: 1005–1006.
Gey, G. O. & Thalhimer, W., 1924. J. Am. Med. Assoc. 82: 1609.
Smith, G. L. & Temin, H. M., 1974. J. Cell Physiol. 84: 181–192.
Rechler, M. M., Podskalny, J. M., Goldfine, I. D. & Wells, C. A., 1974. J. Clin. Endocrinol. Metab. 39: 512–521.
Chochinov, R. H. & Daughaday, W. H., 1976. Diabetes 25: 994–1007.
Bucher, N. L. R. & Weir, G. C., 1976. Metab. Clin. Exp. 25: 1423–1425.
Price, J. B. Jr., 1976. Metab. Clin. Exp. 25: 1427–1428.
Richman, R. A., Claus, T. H., Pilkis, S. J. & Friedman, D. L., 1976. Proc. Natl. Acad. Sci. USA 73: 3589–3593.
Koontz, J. W. & Iwahashi, M., 1980. Science 211: 947–949.
Steiner, D. F., 1966. Vitam. Horm. (N.Y.) 24: 1–61.
Morgan, C. R. & Bonner, J., 1970. Proc. Natl. Acad. Sci. USA 65: 1077–1080.
Krahl, M. E., 1974. Annu. Rev. Physiol. 36: 331–360.
Weber, G., 1972. Isr. J. Med. Sci. 8: 325–343.
Steiner, D. F. & King, J., 1964. J. Biol. Chem. 239: 1292–1298.
Hill, R. E., Lee, K.-L. & Kenney, F. T., 1981. J. Biol. Chem. 256: 1510–1513.
Peavy, D. E., Taylor, J. M. & Jefferson, L. S., 1978. Proc. Natl. Acad. Sci. USA 75: 5879–5883.
Pry, T. A. & Porter, J. W., 1981. Biochem. Biophys. Res. Commun. 100: 1002–1009.
Roy, A. K., Chatterjee, B., Prasad, M. S. K. & Unakar, J. J., 1980. J. Biol. Chem. 255: 11614–11618.
Korc, M., Iwamoto, Y., Sankaran, H., Williams, J. A. & Goldfine, I. D., 1981. Am. J. Physiol. 240 (Gastrointest. Liver Physiol. 3) G56-G62.
Söling, H. D. & Unger, K. O., 1972. Eur. J. Clin. Invest. 2: 199–212.
Korc, M., Owerbach, D., Quinto, C. & Rutter, W. J., 1981. Science 231: 351–353.
Hanse, R. J., Pilkis, S. J. & Krahl, M. E., 1967. Endocrinol. 81: 1397–1404.
Hansen, R. J. & Pilkis, S. J., 1970. Endocrinol. 86: 57–65.
Spooner, P. M., Chernick, S. S., Garrison, M. M. & Scow, R. O., 1979. J. Biol. Chem. 254: 10021–10029.
Stockdale, F. E. & Topper, Y. J., 1966. Proc. Natl. Acad. Sci. USA 56: 1283–1289.
Topper, Y. J., Friedberg, S. H. & Okta, T., 1970. Dev. Biol. (Suppl.) 4: 101–113.
Turkington, R. W., 1968. Endocrinology 82: 540–546.
Terry, P. M., Banerjee, M. R. & Lui, R. M., 1977. Proc. Natl. Acad. Sci. USA 74: 2441–2445.
Bolander, F. F. Jr., Nicholas, K. R., Van Wyk, J. J. & Topper, Y. J., 1981. Proc. Natl. Acad. Sci. USA 78: 5682–5684.
Freychet, P., Roth, J. & Neville, D. M. Jr., 1971. Proc. Natl. Acad. Sci. USA 68: 1833–1837.
Goldfine, I. D., 1979. Receptors in Pharmacology (Smythies, J. R. and Bradley, R. J., eds.), pp. 335–377. Marcel Dekker, New York.
Bergeron, J. J. M., Evans, W. H. & Geschwind, I. I., 1973. J. Cell Biol. 59: 771–776.
Kashnig, D. M. & Kasper, C. B., 1969. J. Biol. Chem. 244: 3786–3792.
Stein, O. & Gross, J., 1959. Endocrinology 65: 707–716.
Goldfine, I. D., Jones, A. L., Hradek, G. T., Wong, K. Y. & Mooney, J. S., 1978. Science 202: 760–762.
Goldfine, I. D., Jones, A. L., Hradek, G. T. & Wong, K. Y., 1981. Endocrinol. 108: 1821–1828.
Franke, W. W., 1974. International Review of Cytology (Bourne, G. H. & Danielli, J. F., eds.) pp. 71–236. Academic Press, New York.
Harris, J. R. & Agutter, P. S., 1976. Biochemical Analysis of Membranes (Maddy, A. H., ed.) pp. 132–173. John Wiley, New York.
Feldherr, C. M., 1972. Adv. Cell and Molec. Biol. 2: 273–281.
Gurr, M. I., Finean, J. B. & Hawthorne, J. N., 1963. Biochim. Biophys. Acta 70: 406–416.
Franke, W. W. & Scheer, U., 1970. J. Ultrastruct. Res. 30: 317–327.
Schumm, D. E. & Webb, T. E., 1978. J. Biol. Chem. 253: 8513–8517.
Clawson, G. A., James, J., Woo, C. H., Friend, D. S., Moody, D. & Smuckler, E. A., 1980. Biochem. 19: 2756–2762.
Agutter, P. S., McCaldin, B. & McArdle, H. J., 1979. Biochem. J. 182: 811–819.
Ishikawa, K., Sato-Odani, S. & Ogata, K., 1978. Biochim. Biophys. Acta 521: 650–661.
Agutter, P. S., 1980. Biochem. J. 188: 91–97.
Lam, K. S. & Kasper, C. B., 1979. Biochem. 18: 307–311.
Monneron, A., Blobel, G. & Palade, G. E., 1972. J. Cell. Biol. 55: 104–125.
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Goldfine, I.D., Clawson, G.A., Smuckler, E.A. et al. Action of insulin at the nuclear envelope. Mol Cell Biochem 48, 3–14 (1991). https://doi.org/10.1007/BF00214816
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DOI: https://doi.org/10.1007/BF00214816