Summary
The isolation and enrichment of the gastric chief cells of the rat are described. Ultrastructural examination showed 85% enrichment from a mixed population of mucosal cells following their centrifugation through a discontinuous Percoll gradient. When compared to homogenates of the initial mixed cell population, the enriched chief cell population showed over a three-fold increase in pepsin(ogen) content. Preliminary experiments showed that a combination of the secretagogues histamine and carbamylcholine caused a significant increase in pepsin release from enriched chief cell preparations and a concomitant decrease in their pepsin content as compared to untreated cells. The results obtained in this study indicate the feasibility of employing this procedure for the isolation of gastric chief cells for the in vitro study of secretagogue regulation of pepsin secretion.
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References
Anson ML, Mirsky AE (1932) The estimation of pepsin with hemoglobin. J Gen Physiol 16:59–63
Berstad A (1970) A modified hemoglobin substrate method for the estimation of pepsin in gastric juice. Scand J Gastroenterol 5:343–348
Daniel WE (1974) Biostatistics, a foundation for analysis in the health sciences. John Wiley and Sons, Inc, New York
Dial E, Thompson WJ, Rosenfeld GC (1981) Isolated parietal cells: histamine response and pharmacology. J Pharmacol Exp Ther 219:585–590
Ecknauer R, Dial E, Thompson WJ, Johnson LR, Rosenfeld GC (1981) Isolated rat gastric parietal cells: cholinergic response and pharmacology. Life Sci 28:609–621
Hirschowitz BI (1967) The control of pepsinogen secretion. Ann NY Acad Sci 140:709–723
Konturek SJ (1980) Secretory mechanisms of acid and pepsin. In: Sircus W, Smith AN (eds) Scientific Foundations of Gastroenterology. WB Saunders Co, Philadelphia Toronto, p 296
Lowry OH, Rosebrough NJ, Farr AL (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Mayhew TM (1979) Isolated peritoneal macrophages: component-biased sampling. In: Weibel ER (ed) Stereological methods. Academic Press, London, Vol 1, p 334
Sutton DR, Donaldson RM (1975) Synthesis and secretion of protein and pepsinogen by rabbit gastric mucosa in organ culture. Gastroenterology 69:166–174
Thompson WJ, Chang LK, Rosenfeld GC (1981) Histamine regulation of adenylyl cyclase of enriched rat gastric parietal cells. Am J Physiol 240:676–684
Wollin A, Soll AH, Samloff FM (1979) Actions of histamine, secretin, and PGE2 on cyclic AMP production by isolated canine fundic mucosal cells. Am J Physiol 237:E437-E443
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This work was supported by USPHS Grant # 20431. Dr. Rosenfeld's work is supported, in part, by Research Career Development Award # AM 00456 from the National Institute of Health
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Fatemi, S.H., Bedrossian, C., Thompson, W.J. et al. Isolation and enrichment of the gastric chief cells of the rat. Cell Tissue Res. 226, 667–674 (1982). https://doi.org/10.1007/BF00214793
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DOI: https://doi.org/10.1007/BF00214793