Skip to main content
SpringerLink
Log in

Molecular and functional diversity of non-histone protein fraction NHCP1 from hamster Kirkman-Robbins hepatoma and liver

  • Original Article
  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

Non-histone protein fraction NHCP1 of micrococcal nuclease-sensitive and nuclease-resistant chromatin from Kirkman-Robbins hepatoma and hamster liver was studied by two-dimensional electrophoresis followed by Coomassie and silver staining and by microcomplement fixation technique in the presence of antibodies elicited against NHCP1 of both tissues. Apart from many common spots several tissue specific components associated with either nuclease-sensitive or nuclease-resistant chromatin were found. The presence of tissue specific components among NHCP1 from hepatoma and liver was confirmed by immunological analysis. It was stated that these components are exclusively localized in nuclease-resistant part of chromatin from neoplastic and normal tissues thus suggesting their structural function.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Elgin SCR, Weintraub H: Chromosomal proteins and chromatin structure. Ann Rev Biochem 44:726–774, 1975.

    Google Scholar 

  2. Stein GS, Stein JL, Thomson JA: Chromosomal proteins in transformed and neoplastic cells: a review. Cancer Res 38:1181–1201, 1978.

    Google Scholar 

  3. Allfrey VG, Boffa LC: Modifications of nuclear protein structure and function during carcinogenesis. In: Busch H (ed). The Cell Nucleus. Academic Press, New York, 1979, pp 521–562.

    Google Scholar 

  4. Phillips IR, Shephard EA, Stein JL, Stein GS: Role of nonhistone chromosomal proteins in selective gene expression. In: Kolodny GM (ed). Eukaryotic Gene Regulation. CRC Press, 1980, vol II, pp 113–177.

  5. Smith GJ: Minireview: Chromatin associated proteins as markers of neoplastic transformation in mammalian cells. Cell Biol Int Rep 5:635–647, 1981.

    Google Scholar 

  6. Stein GS, Plumb M, Stein JL, Phillips IR, Shephard EA: Non-histone proteins in genetic regulation. In: Hnilica LS (ed). Chromosomal nonhistone proteins. CRC Press, Inc, Boca Raton, Florida, 1983, pp 127–186.

    Google Scholar 

  7. Sinclair JH, Rickwood D: Major changes in phosphorylation of chromatin-associated non-histone proteins accompany development in the slime mold Dictyostelium discoideum. Biochem J 229:771–778, 1985.

    Google Scholar 

  8. Dinh B-L, Bourget L: Comparison of nuclear proteins from DMBA-induced mammary tumors and lactating mammary glands by polyacrylamide gel electrophoresis. Breast Cancer Res Treat 5:37–46, 1985.

    Google Scholar 

  9. Bouchonneau M, Durand JP, London J, Pieri J: Two-dimensional gel electrophoresis of low mobility group nonhistone proteins from myeloma cells. Electrophoresis 6:179–184, 1985.

    Google Scholar 

  10. Zardi L, Lin J-C, Baserga R: Immunospecificity to nonhistoric chromosomal proteins of antichromatin antibodies. Nature New Biol 245:211–213, 1973.

    Google Scholar 

  11. Silver IM, Elgin SCR: Immunofluorescent analysis of chromatin structure in relation to gene activity: a speculative assay. Curr Top Dev Biol 13:71–88, 1979.

    Google Scholar 

  12. Bustin M: Immunological approaches to chromatin and chromosome structure and function. Curr Top Microbiol and Immunol 88:105–142, 1979.

    Google Scholar 

  13. Hnilica LS, Briggs RC: Nonhistone protein antigens. In: Sell S (ed). Cancer Markers. The Humana Press, Clifton, 1980, pp 463–483.

    Google Scholar 

  14. Hnilica LS, Schmidt WN, Briggs RC: Nonhistone protein antigens in rat hepatomas. In: Celis JE, Bravo R (eds). Gene Expression in Normal and Transformed Cells. Plenum Publishing Corporation, New York, 1983, pp 415–431.

    Google Scholar 

  15. Busch H, Busch RK, Chan P-K, Kelsey D, Takahashi K: Nucleolar antigens of human tumors. In: Busch H, Yeoman LC (eds). Methods in Cancer Research. Academic Press, New York, 1982, pp 109–178.

    Google Scholar 

  16. Schmidt WN, Hnilica LS: Distribution of Novikoff ascites hepatoma antigens p39 and p49 in various tumorigenic cell lines. Cancer Res 42:1441–1445, 1982.

    Google Scholar 

  17. Schmidt WN, Gronert BJ, McKusick KB, Page DL, Hnilica LS: Cytokeratin and nonhistone protein antigenic changes in rat liver during azo dye but not hepatotoxin feeding. Carcinogenesis 4:675–681, 1983.

    Google Scholar 

  18. Chauveau J, Moulé Y, Rouiller C: Isolation of pure and unaltered liver nuclei, morphology and biochemical composition. Exp Cell Res 11:317–321, 1956.

    Google Scholar 

  19. Lipińska A, Kiliańska Z, Krajewska WM, Kłyszejko-Stefanowicz L: Chromatin proteins associated with micrococcal nuclease-sensitive and nuclease-resistant chromatin fractions of Kirkman-Robbins hepatoma and hamster liver. Molec Biol Rep 10:31–39, 1984.

    Google Scholar 

  20. Kiliańiska Z, Skłodowska M, K⌈yszejko-Stefanowicz L: Characterization of nuclear non-histone proteins from rat liver isolated in the polyethylene glycol-dextran system and separated by hydroxyapatite chromatography. Acta Biochim Polon 27:89–97, 1980.

    Google Scholar 

  21. O'Farrell PH: High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021, 1975.

    Google Scholar 

  22. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685, 1970.

    PubMed  Google Scholar 

  23. Fairbanks J, Steck TL, Wallach DFH: Electrophoresic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10:2606–2617, 1971.

    Google Scholar 

  24. Wray W, Boulikas T, Wray VP, Hancock R: Silver staining of proteins in polyacrylamide gels. Anal Biochem 118:197–203, 1981.

    CAS  PubMed  Google Scholar 

  25. Chytil F, Spelsberg T: Tissue differences in antigenic properties of non-histone protein-DNA complexes. Nature New Biol 233:215–218, 1971.

    Google Scholar 

  26. Chytil F: Immunochemical analysis of nonhistone proteins. In: Stein G, Stein J, Kleinsmith LJ (eds). Methods in Cell Biology. Academic Press, New York, 1977, vol 18, pp 123–141.

    Google Scholar 

  27. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275, 1951.

    CAS  PubMed  Google Scholar 

  28. Burton K: A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 62:315–323, 1956.

    Google Scholar 

  29. Djondjurov LP, Ivanova EC, Tsanev R: Two chromatin fractions with different metabolic properties of nonhistone proteins of newly synthesized RNA. Eur J Biochem 97:133–139, 1979.

    Google Scholar 

  30. Dimitriadis GJ, Tata JR: Subnuclear fractionation by mild micrococcal-nuclease treatment of nuclei of different transcriptional activities caused a partition of expressed and non-expressed genes. Biochem J 187:467–477, 1980.

    Google Scholar 

  31. Weisbrod S: Active chromatin. Nature 297:289–295, 1982.

    Google Scholar 

  32. Tsanev R: Transcriptionally active chromatin. Molec Biol Rep 9:9–17, 1983.

    Google Scholar 

  33. Kiliańiska Z, Kłyszejko-Stefanowicz L: Comparison of nonhistone proteins from hamster Kirkman-Robbins hepatoma and liver. Acta Biochim Polon 32:95–100, 1985.

    Google Scholar 

  34. Heizmann CW, Arnold EM, Kuenzle CC: Fluctuations of non-histone chromosomal proteins in differentiating brain cortex and cerebellar neurone. J Biol Chem 255:11504–11511, 1980.

    Google Scholar 

  35. Defer N, Kitzis A, Levy F, Tichonicky L, Sabatier M-M, Kruh J: Presence of non-histone proteins in nucleosomes. Eur J Biochem 88:583–591, 1978.

    Google Scholar 

  36. Kitzis A, Leibovitch S-A, Leibovitsch M-P, Tichonicky L, Harel J, Kruh J: The small chromatin fragments released by micrococcal nuclease from hepatoma tissue cultured cell nuclei are strongly enriched in coding DNA sequences and are related to an actively transcribed single-stranded DNA fraction. Biochim Biophys Acta 697:60–70, 1982.

    Google Scholar 

  37. Arfmann H-A, Baydoun H: Preferential phosphorylation of high mobility group protein 17 in vitro by nuclear protein kinase. Z Naturforsch 36c:319–322, 1982.

    Google Scholar 

  38. Taylor SS: The in vitro phosphorylation of chromatin by the catalytic subunit of cAMP-dependent protein kinase. J Biol Chem 257:6056–6063, 1982.

    Google Scholar 

  39. Durban E, Roll D, Becker G, Busch H: Purification and characterization of a nuclear-DNA-binding phosphoprotein in fetal and tumor tissues. Cancer Res 41:537–545, 1981.

    Google Scholar 

  40. Dixon DK, Burkholder GD: Fractionation of micrococcal nuclease-digested chromatin solubilized at physiologic ionic strength. Exp Cell Res 156:563–569, 1985.

    Google Scholar 

  41. Lebkowski JS, Laemmli UK: Non-histone proteins and long-range organization of HeLa interphase DNA. J Mol Biol 156:325–344, 1982.

    Google Scholar 

  42. Nelkin B, Pardoll D, Robinson S, Small D, Vogelstein B: Nuclear structure and DNA organization. In: Tumor Cell Heterogeneity. Academic Press, New York, 1982, pp 441–457.

    Google Scholar 

  43. Albrecht J, Hemminki K: Protein composition of chromatin subtractions prepared from chick embryos. Biochim Biophys Acta 475:152–159, 1977.

    Google Scholar 

  44. Kiliańska Z, Kłyszejko-Stefanowicz L: Distribution of nonhistone proteins between micrococcal nuclease sensitive and nuclease resistant chromatin from chicken cells with active and inactive genomes. Cell Biochem Funct 2:78–84, 1984.

    Google Scholar 

  45. Reeves R: Transcriptionally active chromatin. Biochim Biophys Acta 782:343–393, 1984.

    Google Scholar 

  46. Smith GJ, De Luca CM: An analysis of nuclear and chromatin proteins in rat livers exhibiting hyperplastic growth induced by chemical carcinogens. Cancer Biochem Biophys 7:291–299, 1985.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cytobiochemistry, Institute of Biochemistry, University of Łódź, 90-237, Łódź, Poland

    Zofia Kiliańska, Wanda M. Krajewska, Anna Lipińska & Leokadia Kłyszejko-Stefanowicz

Authors
  1. Zofia Kiliańska
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wanda M. Krajewska
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Anna Lipińska
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Leokadia Kłyszejko-Stefanowicz
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kiliańska, Z., Krajewska, W.M., Lipińska, A. et al. Molecular and functional diversity of non-histone protein fraction NHCP1 from hamster Kirkman-Robbins hepatoma and liver. Mol Cell Biochem 71, 167–175 (1986). https://doi.org/10.1007/BF00214776

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00214776

Keywords

Search

Navigation