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The peripheral innervation of the gill of the marine mollusc demonstrated by the aluminium-formaldehyde (ALFA) histofluorescence method

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Summary

The aluminium-formaldehyde (ALFA) histofluorescence method was used to study the innervation of the gill of the marine bivalve mollusc Mytilus edulis and the results were contrasted with those obtained with the standard formaldehyde-induced-fluorescence (FIF) method. The ALFA method produced more fluorescing structures than the FIF method, thus revealing fine branches of the branchial nerve running beneath the gill epithelium which previously remained undetected. This study demonstrates the usefulness of the ALFA histofluorescence method in the study of marine invertebrates.

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Authors and Affiliations

  1. Department of Natural Science, Medgar Evers College, Cuny and The East Coast Neuroscience, Foundation, Inc., USA

    Edward J. Catapane

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  1. Edward J. Catapane
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Additional information

This study was supported in part by Grants 1506RR08171 from NIMH and 5T32GM07641 from the MARC Program of NIGMS. I wish to thank E. Aiello for thoughtful discussions of the work

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Catapane, E.J. The peripheral innervation of the gill of the marine mollusc demonstrated by the aluminium-formaldehyde (ALFA) histofluorescence method. Cell Tissue Res. 225, 449–454 (1982). https://doi.org/10.1007/BF00214696

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  • DOI: https://doi.org/10.1007/BF00214696

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