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Environmental factors affecting neural crest differentiation: Melanocyte differentiation by crest cells exposed to cell-free (deoxycholate-extracted) dermal mesenchyme matrix

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Summary

Deoxycholate-extracted, cell-free matrices were prepared from primary expiants or dispersed cell cultures of embryonic avian dermis, ectoderm, gut mesenchyme, endoderm, pharynx, or umbilical artery. Neural crest cells in association with matrices from dermal expiants or monolayers formed melanocytes after six days. Crest cells in association with matrices from all other tissues or grown on plastic did not form melanocytes. It is concluded that a deoxycholate-resistant structural component of the dermal extracellular matrix induces melanocyte differentiation.

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Abbreviations

ECM:

extracellular matrix

GAG:

glycosaminoglycan

HANKS:

Hank's balanced salt solution

FCS:

fetal calf serum

DME:

Dulbecco's modified Eagle's medium

DOC:

deoxycholate

PMSF:

phenyl methyl sulfonyl fluoride

References

  • Cohen AM, Konigsberg IR (1975) A clonal approach to the problem of neural crest determination. Dev Biol 46:262–280

    Google Scholar 

  • Collins F (1978) Axon initiation by ciliary neurons in culture. Dev Biol 65:50–57

    Google Scholar 

  • Coughlin MD, Bloom EM, Black IB (1981) Characterization of a neuronal growth factor from mouse heart-cell-conditioned medium. Dev Biol 82:56–68

    Google Scholar 

  • Derby MA (1978) Analysis of glycosaminoglycans within the extracellular environments encountered by migrating neural crest cells. Dev Biol 66:321–336

    Google Scholar 

  • Derby MA, Newgreen DF (1982) Differentiation of avian neural crest cells in vitro: Absence of a developmental bias toward melanogenesis. Cell Tissue Res 225:365–377

    Google Scholar 

  • Greenberg JH, Pratt RM (1977) Glycosaminoglycan and glycoprotein synthesis by cranial neural crest cells in vitro. Cell Different 6:119–132

    Google Scholar 

  • Hamburger V, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Morphol 88:49–92

    Google Scholar 

  • Hedman K, Kurkinen M, Alitalo K, Vaheri A, Johansson S, Hook M (1979) Isolation of the pericellular matrix of human fibroblast cultures. J Cell Biol 81:83–91

    Google Scholar 

  • Helfand SL, Smith GA, Wessells NK (1976) Survival and development in culture of dissociated parasympathetic neurons from ciliary ganglia. Dev Biol 50:541–547

    Google Scholar 

  • Holton B, Weston JA (1982) Analysis of glial cell differentiation in peripheral nervous tissue. II. Neurons promote S100 synthesis by purified glial precursor cell populations. Dev Biol 89:72–81

    Google Scholar 

  • Kumegawa M, Nakamura T, Yamada T (1974) Studies on effect of selective membrane filtration on differentiation of tissue culture. J Cell Biol 62:908–911

    Google Scholar 

  • Le Douarin NM (1980) Migration and differentiation of neural crest cells. Current Topics Dev Biol 16:31–85

    Google Scholar 

  • Mayer TC (1973) The migratory pathway of neural crest cells into the skin of mouse embryos. Dev Biol 34:39–46

    Google Scholar 

  • Mayer TC, Oddis L (1977) Pigment cell differentiation in embryonic mouse skin and isolated epidermis: An in vitro study. J Exp Zool 202:415–424

    Google Scholar 

  • Maxwell GD (1976) Cell cycle changes during neural crest differentiation in vitro. Dev Biol 49:66–79

    Google Scholar 

  • Newgreen DF, Theiry J-P (1980) Fibronectin in early avian embryos: Synthesis and distribution along the migration pathways of neural crest. Cell Tissue Res 211:269–291

    Google Scholar 

  • Newgreen DF, Ritterman M, Peters EA (1979) Morphology and behavior of neural crest cells of chick embryo in vitro. Cell Tissue Res 203:115–140

    Google Scholar 

  • Pintar JE (1978) Distribution and synthesis of glycosaminoglycan during quail neural crest morphogenesis. Dev Biol 67:444–464

    Google Scholar 

  • Pratt RM, Larsen MA, Johnston MC (1975) Migration of cranial neural crest cells in a cell-free hyaluronate-rich matrix. Dev Biol 44:298–305

    Google Scholar 

  • Rose GG, Pomerat CM, Shindler TO, Trunnel JB (1958) A cellophane-strip technique for culturing tissue in multipurpose culture chambers. J Biophys Biochem Cytol 4:761–764

    Google Scholar 

  • Weston JA (1970) The migration and differentiation of neural crest cells. Adv Morphogen 8:41–114

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Biological Chemistry, Division of Biology and Biomedical Sciences, Washington University School of Medicine, St. Louis, Missouri, USA

    Michael A. Derby

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  1. Michael A. Derby
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Derby, M.A. Environmental factors affecting neural crest differentiation: Melanocyte differentiation by crest cells exposed to cell-free (deoxycholate-extracted) dermal mesenchyme matrix. Cell Tissue Res. 225, 379–386 (1982). https://doi.org/10.1007/BF00214690

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  • DOI: https://doi.org/10.1007/BF00214690

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