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The effect of reserpine on the pars intermedia of the rat pituitary

An electron-microscopic, fluorescence-histochemical and immunohistochemical study

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Summary

Reserpine has a stimulatory effect on the pars intermedia of the rat pituitary, probably mediated by its action on regulatory catecholaminergic nerves. The effect of single intraperitoneal injections of 0.1–20 mg/kg b.w. of reserpine was studied in adult male rats. Reserpine at a dose of 2 mg/kg b.w. induced degranulation, orientation of the secretory granules along the cell membrane and loss of formaldehyde-chloral-induced fluorescence, accompanied by an activation of the granular endoplasmic reticulum and the Golgi apparatus. With higher doses progressive degranulation and loss of fluorescence were observed. The effect was, however, heterogeneous, and with all doses cells displaying normal ultrastructure and normal fluorescence were regularly present.

To study the release of granular products (containing a different components of the pro-opiomelanocortin chain) from individual cells, formaldehyde-chloral induced fluorescence and α-MSH- and β-endorphin immunoreactivies were demonstrated in consecutive sections from pituitaries of rats given 8 mg/kg body weight of reserpine 24 h before sacrifice. The results indicate coordinated release of these granular products at the cellular level after reserpine treatment.

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References

  • Bäck N, Hervonen H, Rechardt L (1982) Synthesis and storage of hormonal granules in cultured cells of the rat pituitary pars intermedia. Acta Endocrinol 101:507–516

    Google Scholar 

  • Baizman ER, Cox BM, Osman OH, Godstein A (1979) Experimental alterations of endorphin levels in rat pituitary. Neuroendocrinology 28:402–424

    Google Scholar 

  • Balzer H, Makinose M, Hasselbach W (1968) The inhibition of the sarcoplasmic calcium pump by prenylamine, reserpine, chlorpromazine and imipramine. Naunyn-Schmiedebergs Arch Pharmakol Exp Pathol 260:444–455

    Google Scholar 

  • Beckwith BE, Sandman CA (1978) Behavioral influences of the neuropeptides ACTH and MSH: A methodological review. Neurosci Biobehav Rev 2:311–338

    Google Scholar 

  • Bloom F, Battenberg E, Rossier J, Ling N, Leppäluoto J, Vargo TM, Guillemin R (1977) Endorphins are located in the intermediate and anterior lobes of the pituitary gland, not in the neurohypophysis. Life Sci 20:43–48

    Google Scholar 

  • Cote TE, Eskay RL, Frey EA, Grewe CW, Munemura M, Stoof JC, Tsuruta K, Kebabian JW (1982) Biochemical and physiological studies of the beta-adrenoreceptor and the D-2 dopamine receptor in the intermediate lobe of the rat pituitary gland: A review. Neuroendocrinology 35:217–224

    Google Scholar 

  • Denef C, Hautekeete E, de Wolf A, Vanderschueren B (1978) Pituitary basophils from immature male and female rats: Distribution of gonadotrophs and thyrotrophs as studied by unit gravity sedimentation. Endocrinology 103:724–735

    Google Scholar 

  • Denef C, Hautekeete, E, Dewals R, de Wolf A (1980) Differential control of luteinizing hormone and follicle-stimulating hormone secretion by androgens in rat pituitary cells in culture: functional diversity of subpopulations separated by unit gravity sedimentation. Endocrinology 106:724–729

    Google Scholar 

  • Dunn JD, Kastin AJ, Carrillo AJ, Schally AV (1972) Additional evidence for dissociation of melanocyte-stimulating hormone and corticotrophin release. J Endocrinol 55:463–464

    Google Scholar 

  • Fazekas I, Bácsy E, Rappay G (1978) Monolayer cultures of rat pituitary intermediate lobe: growth and fine structure of epithelial cells. Acta Biol Acad Sci Hung 29:273–282

    Google Scholar 

  • Guillemin R, Vargo T, Rossier J, Minick S, Ling N, Rivier C, Vale W, Bloom F (1977) β-endorphin and adrenocorticotropin are secreted concomitantly be the pituitary gland. Science 197:1367–1369

    Google Scholar 

  • Hadley ME, Davis MD, Morgan CM (1977) Cellular control of melanocyte stimulating hormone secretion. Front Horm Res 4:94–104

    Google Scholar 

  • Håkanson R, Sundler P, Larsson LI, Ekman R, Sjöberg NO (1976) Peptides with NH2-terminal tryptophan in adrenocorticotrophin hormone and melanocyte-stimulating hormone granules of adenohypophysis. J Histochem Cytochem 23:65–74

    Google Scholar 

  • Håkanson R, Ekman R, Sundler P, Nilsson R (1980) A novel fragment of the corticotropin-β-lipotropin precursor. Nature 283:789–792

    Google Scholar 

  • Howe A, Thody AJ (1969) The effect of hypothalamic lesions on the melanocyte-stimulating hormone content and histology of the pars intermedia of the rat pituitary gland. J Physiol 203:159–171

    Google Scholar 

  • Howe A, Thody AJ (1970) The effect of ingestion of hypertonic saline on the melanocyte-stimulating hormone content and histology of the pars intermedia of the rat pituitary gland. J Endocrinol 46:201–208

    Google Scholar 

  • Jackson S, Lowry PL (1983) Secretion of pro-opiocortin peptides from isolated perfused rat pars intermedia cells. Neuroendocrinology 37:248–257

    Google Scholar 

  • Kastin AJ, Schally AV, Viosca S, Barrett L, Redding TW (1967a) MSH activity in the pituitaries of rats exposed to constant illumination. Neuroendocrinology 2:257–262

    Google Scholar 

  • Kastin AJ, Arimura A, Viosca S, Barrett L, Schally AV (1967b) MSH activity in pituitaries of rats exposed to stress. Neuroendocrinology 2:200–208

    Google Scholar 

  • Kastin AJ, Beach GD, Hawley WD, Kendall JW, Jr, Edwards MS, Schally AV (1973) Dissociation of MSH and ACTH release in man. J Clin Endocrinel Metab 36:770–772

    Google Scholar 

  • Login IS, MacLeod RM (1981) The direct effect of reserpine in vitro on prolactin release from rat anterior pituitary glands. Brain Res 204:79–85

    Google Scholar 

  • Martin R, Weber E, Voigt KH (1979) Localization of corticotropin- and endorphin-related peptides in the intermediate lobe of the rat pituitary. Cell Tissue Res 196:307–319

    Google Scholar 

  • Masuoka DT, Alcaraz AF, Schott HF (1982) (3H)dopamine release by d-amphetamine from striatal synaptosomes of reserpinized rats. Biochem Pharmacol 31:1969–1974

    Google Scholar 

  • Moriarty GV, Halmi NS, Moriarty CM (1975) The effect of stress on the cytology and immunocytochemistry of pars intermedia cells in the rat pituitary. Endocrinology 96:1426–1436

    Google Scholar 

  • Mueller GP (1981) Beta-endorphin immunoreactivity in rat plasma: variations in response to different physical stimuli. Life Sci 29:1669–1674

    Google Scholar 

  • O'Donohue TL, Dorsa DM (1982) The opiomelanotropinergic neuronal and endocrine systems. Peptides 3:353–395

    Google Scholar 

  • Partanen S (1978) Carbonyl compound induced fluorescence of biogenic monoamines in the endocrine cells of the hypophysis. Progr Histochem Cytochem 10:1–47

    Google Scholar 

  • Partanen S, Bäck N (1979) Quantitative fluorescence histochemistry of combined formaldehyde-chloral-induced fluorescence of aminoterminal tryptophyl-peptide in model experiments and in the pars intermedia of the rat hypophysis. Histochemistry 61:291–300

    Google Scholar 

  • Ploem JS (1971) The microscopic differentiation of the colour of formaldehyde-induced fluorescence. Progr Brain Res 34:27–38

    Google Scholar 

  • Randle JCR, Moor BCM, Kraicer J (1983) Differential control of the release of pro-opiomelanocortin-derived peptides from the pars intermedia of the rat pituitary. Neuroendocrinology 37:131–140

    Google Scholar 

  • Rechardt L, Bäck N (1977) Observations on the depletion of hormone granules from the pars intermedia of the rat pituitary. Acta Anat 99:306

    Google Scholar 

  • Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–213

    Article  CAS  PubMed  Google Scholar 

  • Rodgers RL, Lopaschuk GD, McNeill JH (1983) Effect of reserpine pretreatment on calcium transport and Ca+ +-Mg+ + adenosine triphosphatase activity of guinea-pig cardiac microsomes. J Pharmacol Exp Ther 226:206–210

    Google Scholar 

  • Rosa PA, Policastro P, Herbert E (1980) A cellular basis for the differences in regulation of synthesis and secretion of ACTH/endophin peptides in anterior and intermediate lobes of the pituitary. J Exp Biol 89:215–237

    Google Scholar 

  • Saland LC (1978) Effects of reserpine administration on the fine structure of the rat pars intermedia. Cell Tissue Res 194:115–123

    Google Scholar 

  • Santolaya RC, Ciocca D (1981) Effects of pimozide on the ultrastructure of the pars intermedia in the rat. Cell Tissue Res 217:397–403

    Google Scholar 

  • Schmitt G, Briaud B, Mialhe C, Stutinsky F (1979) Different effects of K+ and Ca+ + on α-MSH and ACTH release from superfused neurointermediate lobe of the rat hypophysis. Neuroendocrinology 28:297–301

    Google Scholar 

  • Schmitt G, Stoeckel ME, Klein MJ, Porte A (1982) Effects of experimental hypoor hypernatremia on the fine structure of the pars intermedia of the murine pituitary. Cell Tissue Res 223:641–657

    Google Scholar 

  • Snyder J, Wilfinger W, Hymer WC (1976) Maintenance of separated rat pituitary mammotrophs in cell culture. Endocrinology 98:25–32

    Google Scholar 

  • Snyder G, Hymer WC, Snyder J (1977) Functional heterogeneity in somatotrophs isolated from the rat anterior pituitary. Endocrinology 101:788–799

    Google Scholar 

  • Sternberger LA (1979) Immunocytochemistry. John Wiley & Sons, New York

    Google Scholar 

  • Taleisnik S, Orias R (1966) Pituitary melanocyte-stimulating hormone (MSH) after suckling stimulus. Endocrinology 78:522–526

    Google Scholar 

  • Taleisnik S, Tomatis ME (1968) Effect of copulation or vaginal stimulation on melanocyte-stimulating hormone content of the hypophysis. Experientia 24:143–144

    Google Scholar 

  • Tomatis ME, Taleisnik S (1968) Influence of reserpine on the pituitary contents of melanocyte-stimulating hormone and on hypothalamic factors which affect its release. J Endocrinol 42:505–512

    Google Scholar 

  • Usategui R, Oliver C, Vaudry H, Lombardi G, Rosenberg I, Mourre AM (1976) Immunoreactive α-MSH and ACTH levels in rat plasma and pituitary. Endocrinology 98:189–196

    Google Scholar 

  • Watson ML (1958) Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol 4:475–478

    Google Scholar 

  • Zallakian M, Knoth J, Metropoulos GE, Njus D (1982) Multiple effects of reserpine on chromaffin-granule membranes. Biochemistry 21:1051–1055

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Anatomy, University of Helsinki, Helsinki, Finland

    Nils Bäck & Leena Rechardt

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  1. Nils Bäck
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  2. Leena Rechardt
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This work was supported by Finska Läkaresällskapet

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Bäck, N., Rechardt, L. The effect of reserpine on the pars intermedia of the rat pituitary. Cell Tissue Res. 241, 1–8 (1985). https://doi.org/10.1007/BF00214619

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  • DOI: https://doi.org/10.1007/BF00214619

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