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A pilot study of lead and cadmium exposure in young children in Stockholm, Sweden: Methodological considerations using capillary blood microsampling

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Abstract

A capillary blood microsampling technique was tested among urban young children in Stockholm. Blood lead (BPb) and hemoglobin (Hb) concentrations were determined in capillary blood obtained by fingerstick from 41 children, 13–20 months old, and the accompanying parent. The quality control included control for lead (Pb) and cadmium (Cd) contamination of material and equipment used for blood sampling, washing procedures for the hands and fingers to be punctured, comparisons of Pb and Cd concentrations in blood obtained by fingerstick and by brachial vein puncture from the same individuals, analysis of external quality control samples for Pb and Cd in blood together with the collected samples, and evaluation of the analytical performance using linear regression analysis.

The results showed that blood sampling material may contaminate the blood samples with amounts of Pb and Cd that would seriously influence the monitoring results in the low concentration range (<100 μg Pb/L and <1 μg Cd/L). However, it is possible to obtain reliable BPb concentrations (>10 μg Pb/L), but not BCd concentrations (<1 μg Cd/L), with the capillary blood microsampling technique tested provided that a strict quality control is applied. The sampling procedure tested was well accepted by the children and their parents. The children's median BPb concentration (27 μg/L; range 9–73 μg/L) was similar to the median BPb concentration of their parents (27 μg/L; range 7–74 μg/L). However, the correlation between child and parent BPb concentrations was poor (R2=0.20), which may indicate different sources to Pb exposure in children and parents.

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References

  • Baghurst PA, Tong S-L, McMichael AJ, Robertson EF, Wigg NR, Vimpani GV (1992a) Determinants of blood lead concentrations to age 5 years in a birth cohort study on children living in the lead smelting city of Port Pirie and surrounding areas. Arch Environ Health 47:203–210

    Google Scholar 

  • Baghurst PA, McMichael AJ, Wigg NR, Vimpani GV, Robertson EF, Roberts RJ, Tong S-L (1992b) Environmental exposure to lead and children's intelligence at the age of seven years. N Engl J Med 327:1279–1284

    Google Scholar 

  • Barltrop D, Smith A (1971) Interaction of lead with erythrocytes. Experienta 27:326–327

    Google Scholar 

  • Bellinger D, Leviton A, Waternaux C, Needleman H, Rabinowitz M (1987) Longitudinal analyses of prenatal and postnatal lead exposure and early cognitive development. N Engl J Med 316:1037–1043

    Google Scholar 

  • Bellinger D, Sloman J, Leviton A, Rabinowitz M, Needleman H, Waternaux C (1991) Low-level lead exposure and children's cognitive function in the preschool years. Pediatrics 87:219–227

    Google Scholar 

  • Bellinger D, Stiles K, Needleman H (1992) Low-level lead exposure, intelligence and academic achievement: A long-term follow-up study. Pediatrics 90:855–861

    Google Scholar 

  • Berglund M, Lind B, Vahter M (1989) UNEP/WHO Human exposure assessment location (HEAL) pilot project on lead and cadmium. In: Total exposure assessment methodology—A new horizon. Proceedings of the EPA/A&WMA Specialty Conference. Air & Waste Management Association and U.S. Environmental Protection Agency, Las Vegas, NV

    Google Scholar 

  • Bjerre B, Berglund M, Harsbo K, Hellman B (1993) Blood lead concentrations of Swedish preschool children in a community with high lead levels from mine waste in soil and dust. Scand J Work Environ Health 19:154–161

    Google Scholar 

  • Bratzel MP, Reed AJ (1974) Microsampling for blood-lead analysis. Clin Chem 20:217–221

    Google Scholar 

  • Bruaux P, Svartengren M (1985) Assessment of human exposure to lead: Comparison between Belgium, Malta, Mexico and Sweden. Report prepared for UNEP/WHO. National Swedish Institute of Environmental Medicine and Department of Environmental Hygiene, Karolinska Institute, Stockholm, and Institute of Hygiene and Epidemiology, Ministry of Health, Brussels

    Google Scholar 

  • CDC (1991) Preventing lead poisoning in young children: A statement by the Centers for Disease Control. Centers for Disease Control, Atlanta, GA

    Google Scholar 

  • Cooney GH, Bell A, McBride W, Carter C (1989) Low-level exposures to lead: The Sydney lead study. Dev Med Child Neurol 31:640–649

    Google Scholar 

  • Crocetti AF, Mushak P, Schwartz J (1990) Determination of numbers of lead-exposed U.S. children by areas of the United States: an integrated summary report of a report to the U.S. Congress on childhood lead poisoning. Environ Hlth Perspec 89:109–120

    Google Scholar 

  • Davis JM (1990) Risk assessment of the developmental neurotoxicity of lead. Neurotoxicology 11:285–292

    Google Scholar 

  • Dietrich KN, Succop PA, Berger OG, Hammond PB, Bornschein RL (1991) Lead exposure and the cognitive development of urban preschool children: the Cincinnati lead study cohort at age 4 years. Neurotoxicol Teratol 13:203–211

    Google Scholar 

  • Dietrich KN, Succop PA, Berger OG, Keith RW (1992) Lead exposure and the central auditory processing abilities and cognitive development of urban children: The Cincinnati lead study cohort at age 5 years. Neurotoxicol Teratol 14:51–56

    Google Scholar 

  • Elinder C-G, Friberg L, Lind B, Nilsson B, Svartengren M, Övermark I (1986) Decreased blood lead levels in residents of Stockholm for the period 1980–1984. Scand J Work Environ Health 12:114–120

    Google Scholar 

  • Fernlund P, Fex G, Hanson A, et al. (1991) Laurells Clinical chemistry in practical medicine (Klinisk kemi i praktisk medicin). Studentlitteratur, Lund, Sweden (in Swedish)

    Google Scholar 

  • IUPAC (1993) Sample collection guidelines for trace elements in: Human whole blood, serum or plasma, packed cells or erythrocytes and urine (Cornelis R, Heinzow B, Herber RFM, Molin Christensen J, Poulsen OM, Sabbioni E, Templeton D, Thomassen Y, Vahter M, Vesterberg O, eds) Technical report. International Union of Pure and Applied Chemistry, Clinical Chemistry Division, Commission of toxicology, Oxford, UK (in press)

  • Jorhem L, Slorach S (1987) Lead, chromium, tin, iron and cadmium in foods in welded cans. Food Addit Contam 4:309–316

    Google Scholar 

  • Juselius R, Lupovich P, Moriarty R (1975) Sampling problems in the micro determination of blood lead. Clin Toxicol 8:53–58

    Google Scholar 

  • Levallois P, Lavoie M, Goulet L, Nantel AJ, Gingras S (1991) Blood lead levels in children and pregnant women living near a lead-reclamation plant. Can Med Assoc J 144:877–885

    Google Scholar 

  • Lind B, Elinder C-G, Friberg L, Nilsson B, Svartengren M, Vahter M (1987) Quality control in the analysis of lead and cadmium in blood. Fresenius Z Anal Chem 326:647–655

    Google Scholar 

  • Lyngbye T, Jörgensen PJ, Grandjean P, Hansen ON (1990) Validity and interpretation of blood lead levels: a study of Danish school children. Scand J Clin Lab Invest 50:441–449

    Google Scholar 

  • Mahaffey KR, Annest JL, Barbano HE, Murphy RS (1979) Preliminary analysis of blood lead concentrations for children and adults: HANES II, 1976–1978. Trace Subst Environ health 13:37–51

    Google Scholar 

  • Meranger JC, Hollebone, BR, Blanchette GA (1981) The effects of storage times, temperatures and container types on the accuracy of atomic absorption determinations of Cd, Cu, Hg, Pb and Zn in whole heparinized blood. J Anal Toxicol 5:33–41

    PubMed  Google Scholar 

  • McMichael AJ, Baghurst PA, Wigg NR, Vimpani GV, Robertson EF, Roberts RJ (1988) Port Pirie cohort study: environmental exposure to lead and children's abilities at the age of four years. N Engl J Med 319:468–475

    Google Scholar 

  • Mielke HW, Heneghan JB (1991) Selected Chemical and physical properties of soils and gut physiological processes that influence lead bioavailability. Chem Speciation Bioavail 3:129–134

    Google Scholar 

  • Moore MR, Meredith PA (1977) The storage of samples for blood and water lead analysis. Clin Chim Acta 75:167–170

    Google Scholar 

  • Nackowski SB, Putnam RD, Robbins DA, Varner MO, White LD, Nelson KW (1977) Trace metal contamination of evacuated blood collection tubes. Am Ind Hyg Assoc J 38:503–508

    Google Scholar 

  • Needleman H, Rabinowitz M, Leviton A, Allred EN (1990) The long-term effects of childhood exposure to low doses of lead: An 11-year follow-up report. N Engl J Med 322:83–88

    CAS  PubMed  Google Scholar 

  • Rabinowitz M, Bellinger D (1988) Soil lead-blood lead relationship among Boston children. Bull Environ Contam Toxicol 41:791–797

    Google Scholar 

  • Schütz A, Attewell R, Skerfving S (1989) Decreasing blood lead in Swedish children, 1978–1988. Arch Environ Health 44:391–394

    Google Scholar 

  • Smith AE (1973a) A study of the variation with pH of the solubility and stability of some metal ions at low concentrations in aqueous solution. Part I. Analyst 98:65–68

    Google Scholar 

  • — (1973b) A study of the variation with pH of the solubility and stability of some metal ions at low concentrations in aqueous solution. Part II. Analyst 98:209–212

    Google Scholar 

  • Stoeppler M, Brandt K (1980) Contributions to automated trace analysis. Part V. Determination of cadmium in whole blood and urine by electrothermal atomic-absorption spectrophotometry. Frezenius Z Anal Chem 300:372–380

    Google Scholar 

  • Stoeppler M, Brandt K, Rains TC (1978) Contributions to automated trace analysis. Part II. Rapid method for the automated determination of lead in whole blood by electrothermal atomic-absorption spectrophotometry. Analyst 103:714–722

    Google Scholar 

  • Thornton I, Davies DJA, Watt JM, Quinn MJ (1990) Lead exposure in young children from dust and soil in the United Kingdom. Environ Health Perspect 89:55–60

    Google Scholar 

  • Vahter M (1982) Assessment of human exposure to lead and cadmium through biological monitoring. National Swedish Institute of Environmental Medicine and Department of Environmental Hygiene, Karolinska institute, Stockholm, Sweden

    Google Scholar 

  • Vahter M, Friberg L (1988) Quality control in integrated human exposure monitoring of lead and cadmium. Fresenius Z Anal Chem 332:726–731

    Google Scholar 

  • Vahter M, Berglund M, Lind B, Jorhem L, Slorach S, Friberg L (1991) Personal monitoring of lead and cadmium exposure—a Swedish study with special reference to methodological aspects. Scand J Work Environ Health 17:65–74

    Google Scholar 

  • Victorin K, Dock L, Vahter M, et al. (1990) Risk assessment of certain compounds in contaminated soil. IEM Report 4/90. Institute of Environmental Medicine, Karolinska institutet, Stockholm, Sweden

    Google Scholar 

  • Weitzman M, Aschengrau A, Bellinger D, Jones R, Hamlin JS, Beiser A (1993) Lead-contaminated soil abatement and urban children's blood lead levels. JAMA 269:1647–1654

    Google Scholar 

  • Ziegler EE, Edwards BB, Jensen RL, Mahaffey KR, Fomon SJ (1978) Absorption and retention of lead by infants. Pediatrics 12:29–34

    Google Scholar 

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Authors and Affiliations

  1. Institute of Environmental Medicine, Karolinska Institute, Box 210, S-171 77, Stockholm, Sweden

    M. Berglund, B. Lind & M. Vahter

  2. Kronan Primary Health Care Center, Sturegatan 4, S-172 31, Sundbyberg, Sweden

    E. Lannerö

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  1. M. Berglund
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  2. B. Lind
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  3. E. Lannerö
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  4. M. Vahter
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Berglund, M., Lind, B., Lannerö, E. et al. A pilot study of lead and cadmium exposure in young children in Stockholm, Sweden: Methodological considerations using capillary blood microsampling. Arch. Environ. Contam. Toxicol. 27, 281–287 (1994). https://doi.org/10.1007/BF00214276

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