Abstract
Acyl-CoA : lysolecithin and lysolecithin : lysolecithin acyltransferases, as well as acyl-CoA hydrolase are important enzymes in lung lipid metabolism. They use amphiphylic lipids as substrates and differ in subcellular localization. In this sense, lipid-protein interactions can be an essential factor in their activity. We have studied the effect of albumin, as lipid-binding protein model, in the activities of these enzymes. Acyl-CoA hydrolase was inhibited in the presence of albumin, whereas acyl-CoA : lysolecithin acyltransferase showed a complex effect of activation depending on both albumin concentration and palmitoyl-CoA/lysolecithin molar ratio. Lysolecithin : lysolecithin acyltransferase was affected differentially on its two activities. Hydrolysis remained unaffected and transacylation was inhibited by albumin. These results are consequence of the interaction of albumin with both lipidic substrates that changes their critical micellar concentration.
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Abbreviations
- TNS:
-
6-(p-toluidino)-2-naphthalene-sulfonic acid
- CMC:
-
Critical Micellar Concentration
- LP:
-
Lysolecithin (1-acyl-sn-glycero-3-phosphocholine)
- PalmCoA:
-
palmitoyl-CoA
References
Infante JR, Huszagh VA: Is there a new biosynthetic pathway for lung surfactant phosphatidylcholine? Trends Biochem Sci 12: 131–133, 1987
Rooney SA: The surfactant system and lung phospholipid biochemistry. Am Rev Respir Dis 131: 439–460, 1985
Wright JR, Clements JA: Metabolism and turnover of lung surfactant. Am Rev Resp Dis 135: 426–444, 1987
Hasegawa-Sasaki H, Ohno K: Acyltransferase activities in rat lung microsomes. Biochim Biophys Acta 380: 486–495, 1975
Oldenborg V, van Golde LMG: Activity of choline phosphotransferase, lysolecithin: lysolecithin acyltransferase and lysolecithin acyltransferase in developing mouse lung. Biochem Biophys Acta 441: 433–442, 1976
Casals C, Acebal C, Cruz-Alvarez M, Estrada P, Arche R: Lysolecithin: lysolecithin acyltransferase from rabbit lung: enzymatic properties and kinetic study.Arch Biochem Biophys 217: 422–433, 1982
Casals C, Acebal C, Pérez-Gil J, Arche R: Effects of lipids on activity and conformation of lysolecithin: lysolecithin acyltransferase from rabbit lung. Mol Cell Biochem 63: 13–20, 1984
Berge RK: Purification and characterization of a long-chain acyl-CoA hydrolase from rat liver microsomes. Biochim Biophys Acta 574: 321–333, 1979
Leibovitz-BenGershon Z, Kobiler I, Gatt S: Lysophospholipases of rat brain. J Biol Chem 247: 6840–6847, 1972
Gatt S, Bartfai T: Rate equations and simulation curves for enzymatic reaction which utilize lipids as substrate. II. Effect of adsorption of the substrate or enzyme on the steadystate kinetics. Biochem Biophys Acta 488: 13–24, 1977
Estrada P, Acebal C, Bauluz C, Casals C, Arche R: Influence of temperature on stability and activity of lysolecithin acyltransferase and acyl-CoA hydrolase from rabbit lung. Biochem Int 8: 339–346, 1984
Barden RE, Cleland WW: 1-Acylglycerol 3-phosphate acyl transferase from rat liver. J Biol Chem 224: 3677–3684, 1969
Constantinides PP, Steim JM: Physical properties of fatty acyl-CoA. J Biol Chem 260: 7573–7580, 1985
Lowry DH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 93: 265–275, 1951
Ross E, Schatz C: Assay of protein in the presence of high concentrations of sulphydryl compounds 54: 304–306, 1973
Haberland ME, Reynolds JA: Interactions of L-a-palmitoyl lysophosphatidylcholine with the AI polypeptide of high density lipoprotein. J Biol Chem 250: 6636–6639, 1975
Wassef MK, Lin YN, Horowitz MI: Phospholipid-deacylating enzymes of rat stomach. Biochim Biophys Acta 528: 318–330, 1978
Berge RK, Shade E, Farstad M: Variations in the activity of microsomel palmitoyl-CoA hydrolase in mixed micelle solutions of palmitoyl-CoA and non-ionic detergents of the Triton X series. Biochim Biophys Acta 666: 25–35, 1981
Arche R, Acebal C, Estrada P, Herrera L: Lysolecithin: lysolecithin acyltransferase from rabbit lung. A conformational study. Int J Peptide Protein Res 23: 487–493, 1984
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Pérez-Gil, J., Estrada, P., Acebal, C. et al. Effect of albumin on acyl-CoA: lysolecithin acyltransferase, lysolecithin : lysolecithin acyltransferase and acyl-CoA hydrolase from rabbit lung. Mol Cell Biochem 94, 167–173 (1990). https://doi.org/10.1007/BF00214123
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DOI: https://doi.org/10.1007/BF00214123