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The demonstration of calcium in the axonal granules of the peripheral nervous system of male Schistosoma mansoni by X-ray microanalysis

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Summary

A conspicuous feature of freeze-dried cryosections of male Schistosoma mansoni is the presence of groups of electron-dense granules. X-ray analysis in the TEM of these granules shows them, in comparison with the adjacent cytoplasm, to contain significantly greater amounts of sodium and calcium. In glutaraldehyde-fixed material, these granules have been correlated with the membrane-bounded, electron-dense granules found within the axons of the peripheral nervous system.

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References

  • Appleton TC (1978) The contribution of cryoultramicrotomy to X-ray microanalysis in biology. In: Erasmus DA (ed) Electron probe microanalysis in biology. Chapman and Hall, London, 148–182

    Google Scholar 

  • Bennett JL, Bueding E (1971) Localization of biogenic amines in Schistosoma mansoni. Comp Biochem Physiol 39(A):859–867

    Google Scholar 

  • Bennett JL, Bueding E, Timms AR, Engstrom RG (1969) Occurrence and levels of 5-hydroxytryptamine in Schistosoma mansoni. Molecular Pharmacol 5:542–545

    Google Scholar 

  • Bloom FE (1973) Ultrastructural identification of catecholamine-containing central synaptic terminals. J Histochem Cytochem 21:333–348

    Google Scholar 

  • Chandler JA (1978) The application of X-ray microanalysis in TEM to the study of ultrathin biological specimens — a review. In: Erasmus DA (ed) Electron probe microanalysis in biology. Chapman and Hall, London, 35–93

    Google Scholar 

  • Chubb IW (1977) The release of non-transmitter substances. In: Cottrell GA, Usherwood PNR (eds) Synapses. Blackie and Son Ltd, Glasgow London, 264–290

    Google Scholar 

  • Dahl G, Ekerdt R, Gratzl M (1979) Models for exocytotic membrane fusion. In: Hopkins CR, Duncan CJ (eds) Secretory mechanisms. Symposia for the Society for Experimental Biology. Cambridge University Press, London New York, Vol XXXIII, 349–368

    Google Scholar 

  • Davies TW, Morgan AJ (1976) The application of X-ray analysis in the transmission electron analytical microscope (TEAM) to quantitative bulk analysis of biological microsamples. J Microscopy 107:47–54

    Google Scholar 

  • Dei-Cass E, Dhainaut-Courtois N, Dhainaut A, Vernes A (1979) Ultrastructural localization of tritiated 5-HT in adult Schistosoma mansoni. A preliminary report. Biol Cellulair 35:321–324

    Google Scholar 

  • Erasmus DA, Davies TW (1979) Schistosoma mansoni and S. haematobium: Calcium metabolisim of the vitelline cell. Exp Parasitol 47:91–106

    Google Scholar 

  • Erasmus DA, Popiel I, Shaw JR (1982) A comparative study of the vitelline cell in Schistosoma mansoni, S. haematobium, S. japonicum and S. mattheei. Parasitol, in press

  • Fetterer R, Pax R, Bennett JL (1980) Praziquantel, potassium and 2,4-dinitrophenol: Analysis of their action on the musculature of Schistosoma mansoni. European J Pharmacol 64:31–38

    Google Scholar 

  • Foreman JC, Garland LG, Mongar JL (1976) The role of calcium in secretory processes: model studies in mast cells. In: Duncan CJ (ed) Calcium in biological systems. Symposia for the Society for Experimental Biology. Cambridge University Press, London New York, Vol XXX, 193–218

    Google Scholar 

  • Matthews EK (1979) Calcium translocation and control mechanisms for endocrine secretion. In: Hopkins CR, Duncan DJ (eds) Secretory mechanisms. Symposia for the Society for Experimental Biology. Cambridge University Press, London New York, Vol XXXIII, 225–249

    Google Scholar 

  • Morgan AJ (1980) Preparation of specimens: Changes in chemical integrity. In: Hayat MA (ed) X-ray microanalysis in biology. University Park Press, Baltimore, 65–165

    Google Scholar 

  • Morgan AJ, Davies TW, Erasmus DA (1978) Specimen preparation. In: Erasmus DA (ed) Electron probe microanalysis in biology. Chapman and Hall, London, 94–147

    Google Scholar 

  • Pax R, Bennett JL, Fetterer R (1978) A benzodiazepine derivative and praziquantel: Effects on musculature of Schistosoma mansoni and Schistosoma japonicum. Archives of Pharmacol 304:309–315

    Google Scholar 

  • Shaw JR, Erasmus DA (1981) Schistosoma mansoni: An examination of the reproductive status of females from single-sex infections. Parasitology 82:121–124

    Google Scholar 

  • Shaw MK, Erasmus DA (1982) Schistosoma mansoni: The presence and ultrastructure of vitelline cells in adult males. J Helminth, in press

  • Stinnakre J (1977) Calcium movements across synaptic membranes and the release of transmitter. In: Cottrell GA, Usherwood PNR (eds) Synapses. Blackie and Son Ltd, Glasgow London, 117–136

    Google Scholar 

  • Winkler H, Smith AD (1975) The chromaffin granule and the storage of catecholamines. In: Blaschko H, Sayers G, Smith AD (eds) Handbook of Physiology. American Physiological Society. Vol VI section 7, 321–339

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Shaw, M.K., Davies, T.W. & Erasmus, D.A. The demonstration of calcium in the axonal granules of the peripheral nervous system of male Schistosoma mansoni by X-ray microanalysis. Cell Tissue Res. 222, 597–604 (1982). https://doi.org/10.1007/BF00213857

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