Abstract
The hyperosmolarity-induced response of the ocular standing potential (SP) provides a method of testing the function of the retinal pigment epithelium (RPE) without using light stimulation.
In this study, the changes in potential level occurring after a short-term intravenous injection of 10 ml of 20% mannitol were determined by means of a direct current amplifier for the following groups: Group 1, normal rabbit eyes; Group 2, rabbit eyes in which the RPE was damaged by sodium iodate; Group 3, rabbit eyes in which the photoreceptors were damaged by monoiodoacetic acid; Group 4, rabbit eyes with uveoretinitis experimentally induced by Arthus-type inflammation.
The following results were obtained:
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1.
The hyperosmolarity-induced SP response consisted of a transient increase in potential level (positive wave) in Group 1.
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2.
For Group 2 a transient decrease in potential level (negative wave) was obtained, i.e., a reversal of the normal positive response to negative wave.
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3.
A positive wave and no reversal was found for Group 3.
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4.
For Group 4 a negative wave and a reversal of the normal response was obtained.
These hyperosmolarity-induced SP responses provide additional information concerning the possibilities of the method for studying the function of the RPE.
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Yamada, M., Suzuki, E., Kikuchi, G. et al. The hyperosmolarity-induced response of the ocular standing potential in mature rabbits. Doc Ophthalmol 66, 347–358 (1987). https://doi.org/10.1007/BF00213663
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DOI: https://doi.org/10.1007/BF00213663