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Effects of trans- and cis-zeatin and optical isomers of synthetic cytokinins on protein kinase activity in vitro

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Abstract

The effects of cis- and trans-zeatin on the activity of the protein kinase associated with barley leaf chromatin were studied. Substances tested were added directly into the incubation medium for enzyme activity estimation. Only trans-zeatin activated the chromatin-associated protein kinase. Maximum activation was detected at a trans-zeatin concentration of 10−9 m. cis-Zeatin had no activity in a range of concentrations from 10−10 to 10−5 m. Comparison of the R-(-)-N 6-1-(1-naphthyl)ethyl-1H-purine-6-amine (R-NEPA) and S-(+)-N 6-(1-naphthyl)ethyl-1H-purine-6-amine (S-NEPA) effects on the enzyme activity showed that only S-NEPA activated the protein kinase from barley leaf chromatin, whereas R-NEPA had no such effect. The data on the effect of other synthetic analogues of cytokinin on the protein kinase activity are also presented. The results are discussed in terms of the specificity and sensitivity of the in vitro response of the chromatin-associated protein kinase from barley leaves to cytokinins. The advantages and limitations of this in vitro assay to test cytokinin activity are also considered.

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Abbreviations

BAP:

N6-benzylaminopurine

R-NEPA:

R(-)-N6-(1-naphthyl)ethyl-1H-purine-6-amine

S-NEPA:

S-(+)-N6-1-(1-naphthyl)ethyl-1H-purine-6-amine

CBP:

cytokinin-binding protein

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya St. 35, 127276, Moscow, Russia

    O. N. Kulaeva & S. Y. Selivankina

  2. Western Regional Center, USDA Agricultural Research Service, 800 Buchanan St., 94710, Albany, California, USA

    J. Corse

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  1. O. N. Kulaeva
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  2. J. Corse
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  3. S. Y. Selivankina
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Kulaeva, O.N., Corse, J. & Selivankina, S.Y. Effects of trans- and cis-zeatin and optical isomers of synthetic cytokinins on protein kinase activity in vitro . J Plant Growth Regul 14, 41–47 (1995). https://doi.org/10.1007/BF00212645

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  • DOI: https://doi.org/10.1007/BF00212645

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