Summary
The distribution in the rat oviduct of γ-aminobutyric acid and its catabolic enzyme GABA-transaminase was studied by the use of immunocytochemical and enzymehistochemical techniques. At the light-microscopic level, both GABA immunoreactivity and GABA-transaminase enzyme reactivity were found primarily in the tubal epithelium while in the muscle layers of the organ only a faint GABA and GABA-transaminase positive staining could be detected. Electron-microscopic evaluation of the GABA immunoreactivity revealed a heavy labelling of the basal bodies (kinetosomes) and a moderate staining of the cilia. These findings indicate that the role of GABA in the oviduct is not related to neurotransmission but may be related to ciliary functions.
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References
Apud JA, Tappaz ML, Celotti F, Negri-Cesi P, Masotto C, Racagni G (1984) Biochemical and immunochemical studies on the GABAergic system in the rat Fallopian tube and ovary. J Neurochem 43:120–125
Erdö SL (1983) Characterization of GABAergic system in the Fallopian tube. Int J Fertil 28:8–9
Erdö SL (1986) GABAergic mechanisms and their possible role in the oviduct and the uterus. In: Erdö SL, Bowery NG (eds) GABAergic mechanisms in the mammalian periphery. Raven Press, New York pp 205–222
Erdö SL, Lapis E (1982) Presence of GABA receptors in rat oviduct. Neurosci Lett 33:275–279
Erdö SL, Rosdy B, Szporny L (1982) Higher GABA concentrations in Fallopian tube than in brain of the rat. J Neurochem 38:1174–1176
Erdö SL, László Á, Szporny L, Zsolnai B (1983) High density of specific GABA binding sites in the human Fallopian tube. Neurosci Lett 42:156–160
Erdö SL, Kiss B, Szporny L (1984a) Comparative characterization of glutamate decarboxylase in crude homogenates of oviduct, ovary and hypothalamus. J Neurochem 43:1532–1537
Erdö SL, Riesz M, Kárpáti E, Szporny L (1984b) GABAB receptor mediated stimulation of the contractility of isolated rabbit oviduct. Eur J Pharmacol 99:333–336
Fernandez I, Orensanz LM, de Ceballos ML (1984) GABA modulation of cholinergic transmission in rat oviduct. Life Sci 35:357–364
Gamrani H, Harandi M, Belin MF, DuBois MP, Calas A (1984) Direct electron microscopic evidence for the coexistence of GABA uptake and endogenous serotonin in the same rat central neurons by coupled radioautographic and immunocytochemical procedures. Neurosci Lett 45:25–30
Hodgson AJ, Penke B, Erdei A, Chubb IW, Somogyi P (1985) Antisera to γ-aminobutyric acid: I. Production and characterization using a new model system. J Histochem Cytochem 33:229–240
Jessen KR, Hills JM, Dennison ME, Mirski R (1983) γ-Aminobutyrate as an autonomic neurotransmitter: Release and uptake of [3H] γ-aminobutyrate in the guinea pig large intestine and cultured enteric neurons using physiological methods and electron microscopic autoradiography. Neuroscience 10:1427–1442
Krnjevic K (1974) Chemical nature of synaptic transmission in vertebrates. Physiol Rev 54:418–540
Martin del Rio R (1981) γ-Aminobutyric acid system in rat oviduct. J Biol Chem 256:9816–9819
Pesetsky I, Burkart J, Hamburg M (1973) Cerebellar development: Histochemical studies of succinic semialdehyde dehydrogenase and γ-aminobutyric acid transaminase in euthyroid, hyperthyroid and hypothyroid rats. Anat Rec 175:411–417
Somogyi P, Hodgson AJ, Chubb IW, Penke B, Erdei A (1985) Antiserum to γ-aminobutyric acid: II. Immunocytochemical application to the central nervous system. J Histochem Cytochem 33:240–248
Sternberger LA (1979) Immunocytochemistry, 2nd edition, Wiley and Sons, New York
Van Gelder NM (1965) The histochemical demonstration of γ- aminobutyric acid metabolism by reduction of tetrazolium salts. J Neurochem 12:231–237
Vincent SR, Kimura H, McGeer EG (1980) The pharmacohisto-chemical demonstration of GABA transaminase. Neurosci Lett 16:345–348
Wu JY (1982) Characterization of L-glutamate decarboxylase in neural and non-neural tissues. In: Okada Y, Roberts E (eds) Problems in GABA research from brain to bacteria. Excerpta Medica, Amsterdam, Oxford, Princeton
Zucker C, Yazulla S, Wu JY (1984) Non-correspondence of [3H] GABA uptake and GAD localization in goldfish amacrine cells. Brain Res 298:154–158
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Erdö, S.L., Somogyi, J., Hámori, J. et al. Light- and electron-microscopic visualization of γ-aminobutyric acid and GABA-transaminase in the oviduct of rats. Cell Tissue Res. 244, 621–626 (1986). https://doi.org/10.1007/BF00212542
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DOI: https://doi.org/10.1007/BF00212542