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The carbamate insecticide ZZ-Aphox® induced structural changes of gills, liver, gall-bladder, heart, and notochord of Rana perezi tadpoles

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Abstract

Embryos of Rana perezi were kept under laboratory conditions and treated with carbamate ZZ-Aphox® at chronic doses of 0.02‰ and 0.14‰ for 9 weeks. Both the histological study and the analysis of mortality show a direct relationship between the dosage and the effects of the pesticide. The histological study of the survivors over 56 days show damages in gills, liver, gall-bladder, heart, and notochord. Damages on the epithelia of gills (on their distal portion) and gall-bladder recover over a few days, whereas those provoked on the compacting of the hepatic parenchyma and the hepatocytes, the auricle and the perinotochordal collagenic fibers alter their structure in a lasting way. Potentials of such alterations are discussed, with special reference to the possible interference of the pesticide on the successful synthesis of the supporting connective sheaths.

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References

  • Alvarez R (1988) Influencia del fotoperiodo y de la glándula pineal sobre el sistema immune de Rana perezi. Resumen tesis doctoral. Publicaciones de la Universidad de León

  • Antunes-Madeira MC, Carvalho AP, Madeira VMC (1981) Interactions of insecticides with erythrocyte membranes. Pestic Biochem Physiol 15(1):79–89

    Google Scholar 

  • Brown HR, Sharma ML (1976) Synaptosomal adenosine triphosphatase (ATP-ase) inhibition by organophosphates. Experientia 32(12):1540–1544

    Google Scholar 

  • Cossarini-Dunier M, Demael A, Siwicki AK (1990) In vivo effect of the organophosphorous insecticide Tricholrphon on the immune response of carp (Cyprinus carpio). Ecotoxicol Environ Saf 19:93–98

    Google Scholar 

  • Dawson DA, McCormick CA, Bantle JA (1985) Detection of teratogenic substances in acidic mine water samples using the frog embryo teratogenesis assay-Xenopus (FETAX). J Appl Toxicol 5(4):234–243

    Google Scholar 

  • Deli E, Kiss Z (1988) Effect of parathion and methylparathion on protein content of chicken embryo muscle in vivo. Biochem Pharmacol 37(17):3251–3256

    Google Scholar 

  • Domenech CE, Domenech EM, Balengo HF, Mendoza D, Farias RN (1977) Pesticide action and membrane fluidity: Allosteric behavior of rat erythrocyte membrane-bound acetylcholinesterase in the presence of organophosphorus compounds. FEBS Lett 74(2):243–246

    Google Scholar 

  • Edery H, Schatzberg-Porath G (1960) Studies on the effects of organophosphorus insecticides on amphibians. Arch Int Pharmacodyn 124:212–224

    Google Scholar 

  • Fawcett DW (1987) Tratado de histología. Ed. Interamericana-McGraw Hill, Madrid

    Google Scholar 

  • Fikes JD (1990) Organophosphorous and carbamate insecticides. Vet Clin North Am: Small Anim Pract 20(2):353–367

    Google Scholar 

  • Garrison JC, Wyttenbach CR (1985) Teratogenic effects of the organophosphate insecticide dicrotophos (Bidrin): Histological characterization of defects. Anat Rec 213:464–472

    Google Scholar 

  • Ghosh P, Bhattacharya S, Bhattacharya S (1989) Impact of non lethal levels of Metacid-50 and Carbaryl on thyroid function and cholinergic system of Chana punctatus. Biomed Environ Sci 2:92–97

    Google Scholar 

  • Gosner KL (1960) A simplified table for staging anuran embryos and larvae with notes on identification. Herpetologica 16:183–190

    Google Scholar 

  • Hall RJ, Kolbe E (1980) Bioconcentration of organophosphorous insecticides to hazardous levels by amphibians. J Toxicol Environ Healt 6:853–860

    Google Scholar 

  • Hanke W, Lange C, Weindel K (1990) The metabolic situation of the liver during development in respect to corticosteroid function. In: Hanke W (ed) Biology and Physiology of amphibians. Gustav Fischer Verlag 1990, Stuttgart, pp 167–176

    Google Scholar 

  • Haya K (1989) Toxicity of pyrethroid insecticides to fish. Environ. Toxicol Chem 8:381–391

    Google Scholar 

  • Honegger RE (1978) Amphibiens el reptiles menacés en Europe. Conseil de l'Europe 15, Strasbourg

    Google Scholar 

  • Huckle KR, Millburn P (1990) Metabolism, bioconcentration and toxicity of pesticides in fish. In: Hutson DH, Roberts TR (eds) Environmental fate of pesticides. John Wiley and Sons, Chichester, 1990, pp 175–244

    Google Scholar 

  • Laurent P, Perry SF (1991) Environmental effects on fish gill morphology. Physiol Zool 64(1):4–25

    Google Scholar 

  • Lima SL, Agostinho CA (1984) Técnicas e propostas para alimentaçao de rãs. Universidade Federal de Viçosa, Informe Técnico 50, Viçosa

    Google Scholar 

  • Marian MP, Arul V, Pandian TJ (1983) Acute and chronic effects of Carbaryl or survival, growth and metamorphosis in the bullfrog Rana tigrina. Arch Envirom Contam Toxicol 12:217–275

    Google Scholar 

  • Meeks RL (1968) The accumulation of 36Cl ring-labeled DDT in a fresh water marsh. J Wildl Manage 32:376–398

    Google Scholar 

  • Meerdink GL (1989) Organophosphorous and carbamate insecticide poisoning large animals. Vet Clin North Am: Food Anim Pract 5(2):375–389

    Google Scholar 

  • Meneely CA, Wyttenbach CR (1989) Effects of the organophosphate insecticides diazinon and parathion on bobwhite quail embryos: skeletal defects and acetyl-cholinesterase activity. J Exp Zool 252:60–70

    Google Scholar 

  • Moreno AJM, Madeira VMC (1990) Interference of parathion with mitochondrial bioenergetics. Biochim Biophys Acta 1015:361–367

    Google Scholar 

  • Nayak PL, Bender ML (1978) Organophosphorus compounds as active site-directed inhibitors of lastase. Biochem Biophys Res Commun 83(3):1178–1183

    Google Scholar 

  • Phillips K (1990) Where have all the frogs and toads gone? BioScience 40(6):422–424

    Google Scholar 

  • Powell GVN, De Weese LR, Lamont TG (1982) A field evaluation of frogs as a potential source of secondary organophosphorous insecticide poisoning. Can J Zool 60:2233–2235

    Google Scholar 

  • Richmonds C, Dutta HM (1989) Histophathological changes induced by Malathion in the gills of bluegill Lepomis macrochirus. Bull Environ Toxicol 43:123–130

    Google Scholar 

  • Riggin GW, Schultz TW (1986) Teratogenic effects of Benzoil Hydrazine on frog embryos. Trans Am Microsc Soc 105:197–210

    Google Scholar 

  • Roy PK, Munshi JSD (1991) Malathion induced structural and morphometric changes of gills of a fresh water major carp, Cirrhinus mrigala (Ham.) J Envirom Biol 12(1):79–87

    Google Scholar 

  • Schultz TW, Dumont JN, Epler RG (1985) The embryotoxic and osteolathyrogenic effects of semicarbazide. Toxicology 36(2): 183–198

    Google Scholar 

  • Schuytema GS, Nebeker AV, Griffis WL, Wilson KN (1991) Teratogenesis, toxicity and bioconcentration in frogs exposed to dieldrin. Arch Environ Contam Toxicol 21:332–350

    Google Scholar 

  • Snawder JE, Chambers JE (1989) Toxic and developmental effects of organophosphorous insecticides embryos of the south african clawed frog. J Environ Sci Health B24(3):205–218

    Google Scholar 

  • —, — (1990) Critical time periods and the effect of tryptophan in malathion-induced developmental defects in Xenopus embryos. Life Sci 46:1635–1642

    Google Scholar 

  • Studnicka M (1970) Investigations on the remnants of the phospho-organic compound foschlor in tissues of fishes subjected to the treatment with this preparation. Pol Arch Weter 13(3):107–121

    Google Scholar 

  • Sultatos LG, Shao M, Murphy SD (1984) The role of hepatic biotransformation in mediating the acute toxicity of the phosphorothionate insecticide chlorpyrifos. Toxicol Appl Pharmacol 73:60–68

    Google Scholar 

  • Szubartowska E, Gromysz-Kalkowska K, Wójcik K (1990) Behavior of the formed blood elements in Rana esculenta L. after repeated contacts of the animal with a therapeutic dose of Foschlor. Bull Environ Contam Toxicol 45:796–803

    Google Scholar 

  • Tucker RK, Crabtree DG (1970) Handbook of toxicity of pesticides to wildlife. U.S. Fish and Wildl. Serv Resour Publ 84, Washington, DC

  • Wyttenbach CR, Thompson SC (1985) The effects of the organophosphate insecticide malathion on very young chick embryos: Malformations detected by histological examination. Am J Anat 174: 187–202

    Google Scholar 

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Authors and Affiliations

  1. Dpto. Biología Celular y Anatomía, Universidad de León, 24071, León, Spain

    M. Pilar Honrubia, M. Paz Herráez & Rafael Alvarez

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  1. M. Pilar Honrubia
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  2. M. Paz Herráez
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  3. Rafael Alvarez
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Honrubia, M.P., Herráez, M.P. & Alvarez, R. The carbamate insecticide ZZ-Aphox® induced structural changes of gills, liver, gall-bladder, heart, and notochord of Rana perezi tadpoles. Arch. Environ. Contam. Toxicol. 25, 184–191 (1993). https://doi.org/10.1007/BF00212129

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  • DOI: https://doi.org/10.1007/BF00212129

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