Summary
Two ‘physiological’ buffers for NMR spectroscopy of biological samples are described. They reflect intracellular and extracellular fluids as far as possible, are easy to prepare, and do not lead to any additional 1H NMR resonances.
References
Godt, R.E. and Maughan, D.W. (1988) Am. J. Physiol., 254, C591–604.
Lehninger, A. (1982) Principles of Biochemistry, Worth Publishers Inc., New York, NY.
Seidel, D. and Schmidt-Gayk, H. (1980) In Innere Medizin, 5th ed. (Ed, Schettler, G.) Thieme, Stuttgart, pp. 548–562.
Smith, G.L. and Martell, A.E. (1976) Critical Stability Constants: Inorganic Complexes, Vol. 4, Plenum Press, New York, NY.
Wang, A.C. and Bax, A. (1993) J. Biomol. NMR, 3, 715–720.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Freund, J., Kalbitzer, H.R. Physiological buffers for NMR spectroscopy. J Biomol NMR 5, 321–322 (1995). https://doi.org/10.1007/BF00211760
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00211760