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A flat-coil NMR probe with hydration control of oriented phospholipid bilayer samples

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Summary

A novel flat-coil solid-state NMR probe capable of controlling the hydration of oriented phospholipid bilayers in the course of long-term experiments, is described. Perfect hydration control for at least five days of intense radio-frequency pulsing is demonstrated using 31P NMR of oriented dimyristoylphospha-tidylcholine bilayers. The probe design will be of particular importance for studies of peptides and proteins oriented in lipid bilayers.

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References

  • Bechinger, B., Kim, Y., Chirlian, L.E., Gesell, J., Neumann, J.-M., Montal, M., Tomich, J., Zasloff, M. and Opella, S.J. (1991) J. Biomol. NMR, 1, 167–173.

    Google Scholar 

  • Bechinger, B. and Opella, S.J. (1991) J. Magn. Reson., 95, 585–588.

    Google Scholar 

  • Bechinger, B. and Seelig, J. (1991) Chem. Phys. Lipids, 58, 1–5.

    Google Scholar 

  • Bechinger, B., Zasloff, M. and Opella, S. (1992) Biophys. J., 62, 12–14.

    Google Scholar 

  • Bechinger, B., Zasloff, M. and Opella, S. (1993) Protein Sci., 2, 2077–2084.

    Google Scholar 

  • Cornell, B.A., Separovic, F., Baldassi, A.J. and Smith, R. (1988) Biophys. J., 53, 67–76.

    Google Scholar 

  • DeJongh, H.H.J., Goormaghtigh, E. and Killian, J.A. (1994) Biochemistry, 33, 14521–14528.

    Google Scholar 

  • Griffin, R.G. (1981) Methods Enzymol., 72, 108–174.

    Google Scholar 

  • Huang, H.W. and Wu, Y. (1991) Biophys. J., 60, 1079–1087.

    Google Scholar 

  • Morrison, C. (1993) Biophys. J., 64, 1063–1068.

    Google Scholar 

  • Nicholson, L.K., Moll, F., Mixon, T.E., LoGrasso, P.V., Lay, J.C., and Cross, T.A. (1987) Biochemistry, 26, 6621–6626.

    Google Scholar 

  • Ulrich, A.S., Volke, F. and Watts, A. (1990) Chem. Phys. Lipids, 55, 61–66.

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, University of Aarhus, DK-8000, Aarhus C, Denmark

    N. C. Nielsen, P. Daugaard, V. Langer, J. K. Thomsen & H. J. Jakobsen

  2. Department of Cell Biology, Institute of Anatomy, University of Aarhus, DK-8000, Aarhus C, Denmark

    S. Nielsen

  3. Novo Nordisk A/S, DK-2880, Bagsværd, Denmark

    O. W. Sørensen

Authors
  1. N. C. Nielsen
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  2. P. Daugaard
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  3. V. Langer
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  4. J. K. Thomsen
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  5. S. Nielsen
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  6. O. W. Sørensen
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  7. H. J. Jakobsen
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Nielsen, N.C., Daugaard, P., Langer, V. et al. A flat-coil NMR probe with hydration control of oriented phospholipid bilayer samples. J Biomol NMR 5, 311–314 (1995). https://doi.org/10.1007/BF00211758

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  • DOI: https://doi.org/10.1007/BF00211758

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