Abstract
Among the HLA-DR-specific monoclonal antibodies (mAbs) that have been characterized previously, there is a marked shortage of DRα chain-specific mAbs which stain unfixed cells. This may result from the high degree of sequence similarity between the α1 domains of HLA-DR and H-2E leading to a state of cross-tolerance to DRα in H-2-expressing mice. BALB/b (E-negative) mice were immunized with DR1-transfected mouse L cells. The chain specificity of the resulting DR-specific mAb was determined using a panel of transfectants expressing hybrid mouse/human class II heterodimers. A DRα-specific mAb was generated which was capable of immunoprecipitating DRαβ dimers and inhibiting the anti-DR alloresponse of human T-cell clones. The present study demonstrates that, with the selection of suitable recipient strain, transfectants can be useful in the generation and definition of chain-specific mouse mAbs.
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Palmer, D., Kevany, M., Mackworth-Young, C. et al. Generation and characterization of an HLA-DRα-specific monoclonal antibody using L-cell transfectants expressing human and mouse class II major histocompatibility dimers. Immunogenetics 33, 12–17 (1991). https://doi.org/10.1007/BF00211690
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DOI: https://doi.org/10.1007/BF00211690