Abstract
Because T-cell receptor (Tcr) genes may possibly function as non-major histocompatibility complex (MHC) immune response genes or predispose for autoimmune diseases, it is important to know how these genes are inherited. We found that Bgl I-digested DNA of BALB/c, C3H, DBA/2, and C57BL/6 exhibited restriction enzyme fragment length polymorphisms (RFLPs) for the Tcra-V1, Tcra-V2, Tcra-V4, Tcra-V6, Tcra-V7, Tcra-V8, Tcra-V11, Tcra-122, Tcra-V13, and Tcra-C gene segments. Inheritance of these RFLPs in 669 offspring from (BALB/c × C57BL/6) × BALB/c, (BALB/c × C57BL/6) × C57BL/6, (C57BL/6 × DBA2) × DBA/2, and (C57BL/6 × C3H) × C3H backcrosses was studied. Since we did not find any recombinations in the offspring, Tcra-V and Tcra-C gene segments are tightly linked and inherited as a haplotype. A peculiar finding was that 22 out of 103 (BALB/c × C57BL/6) × BALB/c offspring, heterozygous for Tcra-C, had deleted a C57BL/6 Tcra-V1 band as well as Tcra-V2 and Tcra-V4 bands. As will be discussed, this deletion is probably caused by heterogeneity in the C57BL/6 breeding stock of a commercial supplier. In seven BXD and BXH recombinant inbred strains with known recombinations between the Tcra-C and Es-10 loci, all Tcra-V RFLPs cosegregated with the Tcra-C RFLP. This finding agrees with the conclusion from our backcross studies; namely that Tcra-V and Tcra-C gene segments are tightly linked.
Similar content being viewed by others
References
Babbit, D. P., Allen, P. M., Matsueda, G., Haber, E., and Unanue, E. R.: Binding of immunogenic peptides to Ia histocompatibility molecules. Nature 317: 359–361, 1985
Bailey, D. W.: Recombinant inbred strains. An aid to finding identity, linkage and function of histocompatibility and other genes. Transplantation 11: 325–327, 1971
Banerjee, S., Haqqi, T. M., Harvinder, S. L., Stuart, J. M., and David, C. S.: Susceptibility to collagen-induced arthritis in mice. Exp Med 167: 832–839, 1988
Benacerraf, B. and McDevitt, H. O.: Histocompatibility-linked immune response genes. Science 175: 273–279, 1972
Bill, J., Yagüe, J., Appel, V., White, J., Horn, G., Erlich, H., and Palmer, E.: Molecular genetic analysis of 178 I-Abm12-reactive T cells. J Exp Med 169: 115–133, 1989
Bogen, B., Snodgrass, R., Briand, J. P., and Hannestad, K.: Synthetic peptides and β-chain gene rearrangements reveal a diversified T cell repertoire for lambda light chain third hypervariable region. Eur J Immunol 16: 1379–1384, 1986
Ceci, J. D., Kingsley, D. M., Silan, C. M., Copeland, N. G., and Jenkins, N. A.: An interspecific backcross linkage map of the proximal half of mouse chromosome 14. Genomics 6: 673–678, 1990
Chien, Y., Becker, D. M., Lindsten, T., Okamura, M., Cohen, D. I., and Davis, M. M.: A third type of murine T-cell receptor gene. Nature 312: 31–35, 1984
Chien, Y., Makio, I., Kaplan, K. B., Elliott J. F., and Davis, M. M.: A new T-cell receptor gene located within the alpha locus and expressed early in T-cell differentiation. Nature 327: 677–682, 1987
Crosby, J. L., Bleackley, R. C., and Nadeau, J. H.: A complex of serine protease genes expressed preferentially in cytotoxic T-lymphocytes is closely linked to the T-cell receptor α-and δ-chain genes on mouse chromosome 14. Genomics 6: 252–259, 1990
Dembic, Z., Bannworth, W., Taylor, B. A., and Steinmetz, M.: The gene encoding the T-cell receptor of α-chain maps close to the Np-2 locus on mouse chromosome 14. Nature 314: 271–273, 1985
Elliott, R. W., Samuelson, L. C., Lambert, M. S., and Meisler, M. H.: Assignment of pancreatic ribonuclease gene to chromosome 14. Cytogenet Cell Genet 42: 110–112, 1986
Epstein, R., Sham, G., Womack, J., Yague, J., Palmer, E., and Cohn, M.: The cytotoxic T cell response to the male-specific histocompatibility antigen (H-Y) is controlled by two dominant immune response genes, one in the MHC, the other in the Tcraα-locus. J Exp Med 163: 759–773, 1986
Gibson, D. M., MacLean, S. J., Anctil, D., and Mathieson, B. J.: Recombination between kappa chain genetic markers in the mouse. Immunogenetics 20: 493–501, 1984
Hedrick, S. M., Cohen, D. I., Nielsen, E. A., and Davis, M. M.: Isolation of cDNA clones encoding T cell-specific membrane-associated proteins. Nature 308: 149–153, 1984
Jouvin-Marche, E., Morgado, M. G., Trede, N., Marche, P. N., Couez, D., Hue, I., Gris, C., Malissen, M., and Cazenave, P.-A.: Complexity, polymorphism, and recombination of mouse T-cell receptor α gene families. Immunogenetics 30: 99–104, 1989
Klotz, J. L., Barth, R. K., Kiser, G. L., Hood, L. E., and Kronenberg, M.: Restriction fragment length polymorphisms of the mouse T-cell receptor gene families. Immunogenetics 29: 191–201, 1989
Lehle, G., Kolb, C., Kappen, C., Schüppel, R., Weiler, E., and Krawinkel, V.: A map of VH genes located next to the DH region in the Igh locus of the two congenic IgH-recombinant mouse strains. Eur J Immunol 18: 1275–1281, 1988
Maniatis, T., Fritsch, E. R., and Sambrook, J.: Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1982
Pisetsky, D. S. and Sachs, D. H.: The genetic control of the immune response to staphylococcal nuclease. VI. Recombination between genes determining the A/J anti-nuclease idiotypes and the heavy chain allotype locus. J Exp Med 146: 1603–1612, 1977
Reed, K. C. and Mann, D. A.: Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res 13: 7207–7221, 1985
Riblet, R. and Brodeur, P.: The Igh-V gene repertoire of the mouse. Sinai J Med (NY) 53: 170–174, 1986
Rueff-Juy, D., Drapier, A.-M., and Cazenave, P.-A.: Mapping of Igk-V genes using backcrossed laboratory and wild mice. Immunogenetics 28: 233–239, 1988
Saito, H., Kranz, M. D., Takagaki, Y., Hayday, A. C., Eisen, H. N., and Tonegawa, S.: A third rearranged and expressed gene in a clone of cytotoxic T lymphocytes. Nature 312: 36–40, 1984
Singer, P. A., McEvilly, R. J., Balderas, R. S., Dixon, F. J., and Theofilopoulos, A. N.: T-cell receptor α-chain variable-region haplotypes of normal and autoimmune laboratory mouse strains. Proc Natl Acad Sci USA 85: 7729–7733, 1988
Taylor, B. A.: Recombinant inbred strains. In M. C. Green (ed.): Genetic Variants and Strains of the Laboratory Mouse, pp. 397–407, Fischer, Stuttgart, 1981
Tutter, A. and Riblet, R.: Duplications and deletions of Vh genes in inbred strains of mice. Immunogenetics 28: 125–135, 1988
Weigert, M. and Riblet, R.: The genetic control of antibody variable regions in the mouse. Springer Semin Immunopathol 1: 133–169, 1978
Wilson, R. K., Lai, E., Concannon, P., Barth, R. K., and Hood, L. E.: Structure, organization and polymorphism of murine and human T cell receptor α and β chain gene families. Immunol Rev 101: 150–172, 1988
Womack, J. E., Davisson, M. T., Eicher, E. M., and Kendall, D. A.: Mapping of nucleotide phosphorylase (Np-1) and Esterase 10 (Es-10) on mouse chromosome 14. Biochem Genet 15: 347–355, 1977
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gleditsch, L., Snodgrass, R. & Bogen, B. No recombinations between Tcra-V and Tcra-C gene segments in 669 backcross mice. Immunogenetics 32, 297–303 (1990). https://doi.org/10.1007/BF00211642
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00211642