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Generation of Listeria monocytogenes-specific T cells mediating delayed footpad reaction and protection in neonatally thymectomized mice but not in nude mice

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Abstract

Neonatally thymectomized (NTx) mice, sham-operated control mice and congenitally athymic nude mice were immunized with viable Listeria monocytogenes and their spleen cells examined for the capacity to transfer both delayed footbad reaction and protection against challenge at the site of local transfer. Cells from immune NTx mice conferred significant degrees of delayed footpad reaction and protection comparable to sham mice, while cells from immune nude (nu/nu) mice did not. This abilty was completely eliminated by the treatment of cells with anti-Thy1, anti-Lytl or anti-L3T4 antibody plus complement but not with anti-Lyt2 antibody plus complement. These results indicated that NTx mice can normally mount the immunity to L. monocytogenes by generating Lyt1+2−, L3T4+ T cells. Immune competence of NTx mice and thymus dependency of various immune responses are discussed.

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Authors and Affiliations

  1. Department of Bacteriology, Niigata University School of Medicine, 951, Niigata, Japan

    Masao Mitsuyama

  2. Department of Bacteriology, Faculty of Medicine, Medical Institute of Bioregulation, Kyushu University, 812, Fukuoka, Japan

    Masao Mitsuyama, Masatoshi Sano & Kazunobu Amako

  3. Department of Immunology, Medical Institute of Bioregulation, Kyushu University, 812, Fukuoka, Japan

    Yoshitsugu Watanabe & Kikuo Nomoto

Authors
  1. Masao Mitsuyama
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  2. Yoshitsugu Watanabe
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  3. Masatoshi Sano
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  4. Kazunobu Amako
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  5. Kikuo Nomoto
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Mitsuyama, M., Watanabe, Y., Sano, M. et al. Generation of Listeria monocytogenes-specific T cells mediating delayed footpad reaction and protection in neonatally thymectomized mice but not in nude mice. Med Microbiol Immunol 177, 207–217 (1988). https://doi.org/10.1007/BF00211220

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  • DOI: https://doi.org/10.1007/BF00211220

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