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Cell type-dependent difference in the distribution and frequency of excess thymidine-induced common fragile sites: T lymphocytes and skin fibroblasts

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Summary

Common fragile sites were induced by excess thymidine in phytohemagglutin-stimulated T lymphocytes from 4 normal individuals, and skin fibroblasts from 4 normal and 5 fra(X) positive individuals. The results indicate that the frequency and distribution of excess thymidine-induced fragile sites are different between these two types of cells. The sites at 1p13 and 2p11.2, induced in both types of cells, have not previously been described, and are thus considered to be excess thymidine-specific fragile sites. These findings extend and support our previous studies on cell type-dependent difference in aphidicolin-induced common fragile sites.

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References

  • Kuwano A, Kajii T (1987) Synergistic effect of ethanol and aphidicolin on the induction of common fragile sites. Hum Genet 75:75–78

    Google Scholar 

  • Kuwano A, Sugio Y, Murano I, Kajii T (1988) Common fragile sites induced by folate deprivation, BrdU, and aphidicolin: their frequency and distribution in Japanese individuals. Jpn J Hum Genet 33:355–364

    Google Scholar 

  • Murano I, Kuwano A, Kajii T (1989a) Fibroblast-specific common fragile sites. Hum Genet 83:45–48

    Google Scholar 

  • Murano I, Kuwano A, Kajii T (1989b) Cell type-dependent difference in the distribution and frequency of aphidicolin-induced fragile sites: T and B lymphocytes and bone marrow cells. Hum Genet 84:71–74

    Google Scholar 

  • Sutherland GR, Baker E (1986) Induction of fragile sites in fibroblasts. Am J Hum Genet 38:573–575

    Google Scholar 

  • Sutherland GR, Ledbetter DH (1988) Report of the committee on cytogenetic markers. Cytogenet Cell Genet 49:221–223

    Google Scholar 

  • Sutherland GR, Parslow MI, Baker E (1985a) New classes of common fragile sites induced by 5-azacytidine and bromodeoxyuridine. Hum Genet 69:233–237

    Google Scholar 

  • Sutherland GR, Baker E, Fratini A (1985b) Excess thymidine induces folate sensitive fragile sites. Am J Med Genet 22:433–443

    Google Scholar 

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Authors and Affiliations

  1. Department of Pediatrics, Yamaguchi University School of Medicine, 755, Ube, Japan

    Akira Kuwano, Ichiro Murano & Tadashi Kajii

Authors
  1. Akira Kuwano
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  2. Ichiro Murano
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  3. Tadashi Kajii
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Kuwano, A., Murano, I. & Kajii, T. Cell type-dependent difference in the distribution and frequency of excess thymidine-induced common fragile sites: T lymphocytes and skin fibroblasts. Hum Genet 84, 527–531 (1990). https://doi.org/10.1007/BF00210803

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  • DOI: https://doi.org/10.1007/BF00210803

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