Abstract
The human recombination activating gene 1 (RAG1) has previously been mapped to chromosomes 14q and 11p. Here we confirm the chromosome 11 assignment by two independent approaches: autoradiographic and fluorescence in situ hybridization to metaphase spreads and analysis of human-hamster somatic cell hybrid DNA by the polymerase chain reaction (PCR) and Southern blotting. Our results unequivocally localize RAG1 to 11p13.
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References
Blackwell TK, Alt FW (1988) Immunoglobulin genes. In: Hames BD, Glover DM (eds) Molecular immunology. IRL Press, Washington, DC, pp 61–79
Blanquet V, Turleau C, Goossens M, Besmond C (1992) Assignment of the human recombination activating gene 1 (RAG1) to the 14q21.3-q22.2 region. Genomics 13:488–489
Couillin P, Azoulay M, Henry I, Ravise N, Grisard MC, Jeanpierre C, Barichard F, Metezeau P, Candelier JJ, Lewis W, Heyningen V van, Junien C (1989) Characterization of a panel of somatic cell hybrids for subregional mapping along 11p and within band 11p13. Subdivision of the WAGR complex region. Hum Genet 82:171–178
Davis MM (1988) T-cell antigen receptor genes. In: Hames BD, Glover DM (eds) Molecular immunology. IRL Press, Washington, DC, pp 61–79
Davisson MT, Lalley PA, Peters J, Doolittle AL, Hill RE, Favor J, Hogan BLM, Ton CCT, Saunders GF, Hanson IM, Prosser J, Jordan T, Hastie ND, Heyningen V van (1991) Mouse small eye results from mutations in a paired-like homeobox-containing gene. Nature 354:522–525
Gessler M, Thomas GH, Couillin P, Junien C, McGillivray B, Hayden J, Jaschek G, Bruns GAP (1989) A deletion map of the WAGR region on chromosome 11. Am J Hum Genet 44:486–495
Glaser T, Housman D, Lewis WH, Gerhard D, Jones C (1989) A fine-structure deletion map of human chromosome 11p: analysis of the J1 series hybrids. Somat Cell Mol Genet 15:477–501
Ichihara Y, Hirai M, Kurosawa Y (1992) Sequence and chromosome assignment to 11p13-p12 of human RAG genes. Immunol Lett 33:277–284
Kunze N, Yang GC, Jiang ZY, Hameister H, Adolph S, Wiedorn KH, Richter A, Knippers R (1989) Localization of the active type I DNA topoisomerase gene on human chromosome 20q11.2–13.1 and two pseudogenes on chromosome 1q23–24 and 22q11.2–13.1. Hum Genet 84:6–10
Lichter P, Chang Tang Ch-J, Call K, Hermanson G, Evans GA, Housman D, Ward DC (1990) High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 247:64–69
Luerssen H, Mattei M-G, Schröter M, Grzeschik K-H, Adham IM, Engel W (1990) Nucleotide sequence of the gene for human transition protein 1 and its chromosomal localization on chromosome 2. Genomics 8:324–330
Oettinger MA, Schatz DG, Gorka C, Baltimore D (1990) RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination. Science 248:1517–1523
Oettinger MA, Stanger B, Schatz DG, Glaser T, Call K, Housman D, Baltimore D (1992) The recombination activating genes, RAG1 and RAG2, are on chromosome 11p in humans and chromosome 2p in mice. Immunogenetics 35:97–101
Perry P, Wolff S (1974) New Giemsa method for the differential staining of sister chromatids. Nature 251:156–158
Saiki RK, Gelfand GH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
Schatz DG, Oettinger MA, Baltimore D (1989) The V(D)J recombination activating gene (RAG-1). Cell 59:1035–1048
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Schwarz, K., Hameister, H., Gessler, M. et al. Confirmation of the localization of the human recombination activating gene 1 (RAG1) to chromosome 11p13. Hum Genet 93, 215–217 (1994). https://doi.org/10.1007/BF00210617
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DOI: https://doi.org/10.1007/BF00210617