Abstract
In order to identify the chromosomal assignment for the human indoleamine 2,3-dioxygenase (IDO) gene, we performed the polymerase chain reaction (PCR) using somatic cell hybrids and screened for the presence of amplified products. The results indicate that the human IDO gene can be assigned to chromosome 8.
References
Carlock LR, Smith D, Wasmuth JJ (1986) Genetic counterselective procedure to isolate interspecific cell hybrids containing single human chromosomes: construction of cell hybrids and recombinant DNA libraries specific for human chromosome 3 and 4. Somat Cell Mol Genet 12:163–174
Goto M, Rubenstein M, Weber J, Woods K, Drayna D (1992) Genetic linkage of Werner's syndrome to five markers on chromosome 8. Nature 355:735–738
Kadoya A, Tone S, Maeda H, Minatogawa Y, Kido R (1992) Gene structure of human indoleamine 2,3-dioxygenase. Biochem Biophys Res Commun 189:530–536
Maza LM de la, Peterson EM (1988) Dependence of the vitro antiproliferative activity of recombinant human γ-interferon on the concentration of tryptophan in culture media. Cancer Res 48:346–350
Ozaki Y, Edelstein MP, Duch DS (1988) Induction of indoleamine 2,3-dioxygenase: a mechanism of the antitumor activity of interferon γ. Proc Natl Acad Sci USA 85:1242–1246
Ruano G, Lewis M, Kouri R (1989) Gene mapping with Bios PCRable DNA from somatic cell hybrids: a comprehensive guide for users. Bios Corporation, New Haven, Conn
Slawin K, Sawczuk IS, Olsson CA, Buttyan R (1990) Chromosomal assignment of the human homologue encoding SGP-2. Biochem Biophys Res Commun 172:160–164
Takikawa O, Yoshida R, Kido R, Hayaishi O (1986) Tryptophan degradation in mice initiated by indoleamine 2,3-dioxygenase. J Bio Chem 261:3648–3653
Takikawa O, Kuroiwa T, Yamazaki F, Kido R (1988) Mechanism of interferon-γ action. Characterization of indoleamin 2,3-dioxygenase in cultured human cells induced by interferon-γ and evaluation of the enzyme-mediated tryptophan degradation in its anticellular activity. J Biol Chem 263:2041–2048
Tone S, Takikawa O, Habara-Ohkubo A, Kadoya A, Yoshida R, Kido R (1990) Primary structure of human indoleamine 2,3-dioxygenase deduced from the nucleotide sequence of its cDNA. Nucleic Acids Res 18:367
Werner ER, Hirsch-Kauffmann M, Fuchs D, Hausen A, Reibnegger G, Schweiger M, Wachter H (1988) Interferon-β-induced degradation of tryptophan by human cells in vitro. Biol Chem Hoppe-Seyler 368:1407–1412
Yamazaki F, Kuroiwa T, Takikawa O, Kido R (1985) Human indoleamine 2,3-dioxygenase. Its tissue distribution, and chracterization of the placental enzyme. Biochem J 230:635–638
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tone, S., Kadoya, A., Maeda, H. et al. Assignment of the human indoleamine 2,3-dioxygenase gene to chromosome 8 using the polymerase chain reaction. Hum Genet 93, 201–203 (1994). https://doi.org/10.1007/BF00210611
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00210611