Summary
The fine structure of ADH-induced intramembrane particle aggregates has been studied in different tissues and under different experimental conditions. Particle aggregates similar to those previously observed in the amphibian urinary bladder and in the mammalian collecting duct were also found in the frog skin, another ADH target tissue. In the frog urinary bladder, typical aggregates were observed in the absence of glutaraldehyde fixation. Two experimental approaches were used a) the absence of both fixative and cryoprotectant treatments and b) the absence of only glutaraldehyde treatment. In the latter case the reversal of hydrosmotic action was prevented by exposing the preparations to N-ethyl maleimide. In specimens of frog urinary bladder conventionally fixed with glutaraldehyde, two fracture levels could be observed in the aggregates, suggesting that the aggregated particles span an appreciable part of the membrane thickness.
Résumé
L'ultrastructure des agrégats de particules intramembranaires liés à l'action de l'hormone antidiurétique a été étudiée dans différentes conditions. Des agrégats comparables à ceux décrits précédemment dans la vessie des amphibiens et dans les canaux collecteurs des mammifères, ont également été observés, après stimulation hormonale, dans un autre effecteur de l'hormone antidiurétique, la peau de grenouille. Nous avons par ailleurs observé, dans la vessie de grenouille, des agrégats typiques en absence de fixation par le glutaraldéhyde. Deux protocoles ont été expérimentés a) l'absence de toute fixation ou cryoprotection b) l'absence de la seule fixation par le glutaraldéhyde. Dans ce dernier cas, la dissipation de l'effet hydrosmotique a été évitée par un traitement des préparations par le N-ethylmaleimide. Enfin nous avons pu mettre en évidence, dans certains specimens de vessie de grenouille fixés par le glutaraldéhyde, l'existence de deux niveaux distincts de fracture des particules des agrégats, ce qui suggère que les structures sousjacentes s'étendent transversalement dans la membrane.
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J. Chevalier is a career investigator from the Institut National de la Santé et de la Recherche Médicale, INSERM U 48, France
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Chevalier, J., Adragna, N., Bourguet, J. et al. Fine structure of intramembranous particle aggregates in ADH-treated frog urinary bladder and skin: Influence of glutaraldehyde and N-ethyl maleimide. Cell Tissue Res. 218, 595–606 (1981). https://doi.org/10.1007/BF00210118
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DOI: https://doi.org/10.1007/BF00210118