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Predominance and tissue specificity of adenine methylation in rice

Theoretical and Applied Genetics volume 80, pages 402–408 (1990)Cite this article

Summary

Using ‘A’ and ‘C’ methylation-specific restriction enzymes, namely, MboI, Sau3AI, DpnI, MspI, and HpaII, total rice cv Basmati 370 DNA, repetitive DNAs, and a specific repeat sequence indicated an abundance of adenine methylation. Although cytosine methylation in 5′-CCGG-3′ sequences suggested more CpC methylation than CpG, the ‘C’ methylation in sequence 5′-GATC-3′ was comparatively less than ‘A’ methylation. Furthermore, the presence of adenine methylation was tissue specific; it was predominant in rice shoot DNA as compared to embryo DNA. This pattern was also observed in two other cultivars of rice, i.e., R-24 and Sona, and was again confirmed using a cloned probe of a specific repeat sequence. Besides the changes in adenine methylation, there was also a qualitative change in 5mC from CpG to CpC dinucleotides in these two tissue systems.

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Affiliations

  1. Division of Biochemical Sciences, National Chemical Laboratory, 411008, Pune, India

    M. S. Dhar, V. V. Pethe, V. S. Gupta & P. K. Ranjekar

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  1. M. S. Dhar
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  2. V. V. Pethe
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  3. V. S. Gupta
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  4. P. K. Ranjekar
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Communicated by G. S. Khush

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Dhar, M.S., Pethe, V.V., Gupta, V.S. et al. Predominance and tissue specificity of adenine methylation in rice. Theoret. Appl. Genetics 80, 402–408 (1990). https://doi.org/10.1007/BF00210080

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  • DOI: https://doi.org/10.1007/BF00210080

Key words

  • Rice
  • Adenine methylation
  • Tissue specificity
  • Genomic rearrangement