Abstract
The prothrombin time of the normal human pooled plasma was shortened by Aldrich humic acid well water humic substances and lignin at final concentrations ranging from 5 × 10−3 mg mL−1 to 5 × 10−2 mg mL−1, with a maxmum effect at 1 × 10−2 mg mL−1. At this concentration the shortening was 5.5 s, 4.4 s, and 3.5 s by Aldrich humic acid, well water humic substances and lignin respectively. However, monomeric components of humic acid such as syringic acid, protocatechuic acid, pyrogallol, vanillic acid, gallic acid, resorcinol, ferulic acid, catechol, caffeic acid and p-coumaric acid did not have such ability to shorten prothrombin time at the same final concentration ranges. Certain reducing agents such as t-butanol (2.5 × 10− −2.0 × 10−2 mg mL−1), glutathione (8.0 × 10−2−1 .0 mg mL−1), ascorbic acid (4.2 × 10−2−5.0 × 10−1 mg mL−1) and dithiothreitol (1.0 × 10−2−1.7 × 10−1mg mL−1) could prevent the shortening effects of humic substances or lignin on prothrombin time. These results suggested that humic substances and lignin with a polymerised structure had an ability to affect activities of some blood coagulating enzymes.
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Burges, N. A., Hurst, H. M. and Walkdene, B. 1964. The phenolic constitutents of humic acid and their relationship to the lignin of the plant cover. Geochim Cosmochim Acta, 28 1547–1554.
Choudhry, G. G. 1981. Humic substances Part I: Structure aspects. Toxicology Environmental Chemistry, 4, 209–259.
Cooksey, R. C., Gaitan, E., Lindsay, R. J., Hill, J. R. and Kelly, K. 1985. Humic substances, a possible source of environmental goitrogens. Organic Geochemistry, 8, 77–80.
Gaitan, E., Medina, T. A. and Zia, M. S.. 1980. Goiter prevalence and bacterial concentration of water supply. Journal of Clinical Endocrinology and Metabolism, 51, 957–962.
Gaitan, E., Jolley, R. L., Lee, N. E. Lindsey, R. J., Cooksey, R. C., Hill, J. R. and Kelly, K. 1983. Phthalate ester possible progoitrogens in water supply of a Columbia district with endemic goiter. Journal of American Chemical Society (Divison Environmental Chemistry), 23, 175–178.
Hartenstein, R. 1981. Sludge decomposition and stabilization. Science, 212, 743–749.
Hayastu, R., Winnas, R. E., McBeth, R. L., Scott, R. G., Moore, L. P. and Studier, M. H. 1979. Lignin-like polymers in coals. Nature, 278, 41–43.
Keyser, P., Pujar, B. J. Eaton, R. W. and Ribons, D. W.. 1976. Biodegradation of the phthalates and their esters by bacteria. Environmental Health Perspective, 18, 159–166.
Ladd, J. N. and Butler, J. H. 1969. Inhibitory effect of soil humic compounds on the proteolytic enzyme pronase. Australian Journal Soil Research, 7, 241–251.
Lu, F. J., Yang, C. K. and Lin, K. H. 1975. Physico-chemical characteristics of drinking water in Blackfoot disease endemic areas in Chia-I and Tainan Hsiens. Journal of Formosan Medical Association, 74, 596–605.
Lu, F. J., Yamamura, Y. and Yamauchi, H. 1988. Studies on fluorescent compounds in water of a well in Blackfoot disease endemic areas in Taiwan: Humic substances. Journal of Formosan Medical Association, 87, 66–75.
Lu, F. J. 1990a. Fluorescent humic substances and Blackfoot disease in Taiwan. Applied Organometallic Chemistry, 4, 191–195.
Lu, F. J. 1990b. Blackfoot disease: arsenic or humic acid? Lancet, 336, 115–116.
Lu, F. J., Shih, S. R., Liu, T. M. and Shown, S. H.. 1990. The effect of fluorescent humic substances existing in the well water of blackfoot disease endemic areas in Taiwan on prothrombin time and activated partial thromboplastin time in vitro. Thrombosis Research, 57, 747–753.
Lu, F. J. and Lee, Y. S. 1992. Humic acid: inhibitor of plasmin. The Science of the Total Environment, 114, 135–139.
Malcolm, R. E. and Vaughan, D. 1979a. Humic substances and phosphatase activities in plant tissues. Soil Biology and Biochemistry, 11, 253–259.
Malcolm, R. E. and Vaughan, D. 1979b. Effects of humic acid fractions on invertase activities in plant tissues. Soil Biology and Biochemistry, 11, 65–72.
Marty, J. Y. and Bastide, J. 1982. Fixation de ribonuclease a sur des element du sol. Soil Biology and Biochemistry, 14, 519–522.
Mato, M. C., Olmedo, M. G. and Mendez, J. 1972. Inhibition of indoleacetic acid oxidase by soil humic acid fractionated on Sephadex. Soil Biology and Biochemistry, 4, 269–273.
Pflug, W. and Ziechmann, W. 1981. Inhibition of malate dehydrogenase by humic acids. Soil Biology and Biochemistry, 13, 293–299.
Ratnoff, O. D. and Crum, J. D. 1964. Activation of Hageman factor by solutions of ellagic acid. The Journal of Laboratory and Clinical Medicine, 63, 359–377.
Serban, A. and Nissenbaum, A. 1986. Humic acid association with peroxidase and catalase. Soil Biology and Biochemistry, 18, 41–44.
Tseng, W. P., Chen, W. Y. Sung, J. L. and Chen, J. S. 1961. A clinical study of Blackfoot disease in Taiwan: An endemic peripheral vascular disease. Memorie College of Medicine National Taiwan University, 7, 1–8.
Yang, H. L., Lu, F. J., Wung, S. L. and Chiu, H. C. 1994 Humic acid induces expression of tissue factor by cultured endothelial cells: regulation by cytosolic calcicum and protein kinase C. Thrombosis and Hemostasis, 71, 325–30
Zhai, S. S., Kimbrough, R. D. Meng, B., Han, J. Y. Levoid, M., Hou, X. and Yin, X. N. 1990. Kashin-Beck disease: a cross-sectional study in seven villages in the Peoples' Republic of China. Journal Toxicology Environmental Health, 30, 239–259.
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Lu, FJ., Lin, WW. & Huang, TS. Humic substances shorten human plasma prothrombin time. Environ Geochem Health 16, 65–69 (1994). https://doi.org/10.1007/BF00209826
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DOI: https://doi.org/10.1007/BF00209826