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The ultrastructure of multilamellar bodies and surfactant in the human lung

Cell and Tissue Research volume 193, pages 219–229 (1978)Cite this article

Summary

Normal tissues from human lungs were dehydrated through Epon 812 resin to retain many of the lipids and carbohydrates in thin section. The three-dimensional structure of the multilamellar body was determined. The paired layer of phospholipid heads (PH) is 36Å thick; the layer of fatty-acid tails (FA) is 31Å, the same as reported previously for non-human primates and rodents. The human multilamellar body is apparently unique: the lamellae of the major focus divide into two or three lamellae; the matrix material of the core is without vesicular bodies and a projection core is present. When compared with those of the rat, human tissues contain a greater number of lamellar foci and fewer lamellae per focus. The presence of a peripheral layer of lamellae, an ever-present external limiting membrane, and the fusion of multilamellar bodies are also characteristic. Tubular myelin surfactant has the same appearance as in other mammals.

Multilamellar bodies were observed in direct communication with Golgi vesicles. Their origin from multivesicular bodies and their maturation through secretion and exocytosis were demonstrated.

Untransformed multilamellar bodies in the alveolar space demonstrated three periodicities (P): (1) compact regular lamellae, PH = 36Å, FA = 31Å, P = 66Å; (2) compact broad lamellae, PH = 72Å, FA = 22Å, P = 94Å; (3) loose lamellae, PH = 36Å, FA = 36Å, FA = 31Å with a variable interlamellar space.

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References

  • Bensch, K., Schaefer, K., Avery, M.D.: Granular pneumocytes: Electron microscopic evidence of their exocrinic function. Science (N.Y.) 145, 1318–1319 (1964)

    Google Scholar 

  • Callas, G., DeGroot, W.T.: Lamellar transformation from type II pneumocyte mitochondria in rat lungs. Anat. Rec. 174, 369 (1976)

    Google Scholar 

  • Douglas, W.H.J., Redding, R.A., Stein, M.: The lamellar substructure of osmiophilic inclusion bodies present in rat type II alveolar pneumocytes. Tissue and Cell 7, 137–142 (1975)

    Google Scholar 

  • Hatasa, K., Nakamura, T.: Electron microscopic observation of lung alveolar epithelial cells of normal young mice with special reference to formation and secretion of osmiophilic lamellar bodies. Z. Zellforsch. 68, 266–277 (1965)

    Google Scholar 

  • Kaibara, M., Kikkawa, Y.: Osmiophilia of the saturated phospholipid, dipalmitoyl lecithin, and its relationship to the alveolar lining layer of the mammalian lung. Amer. J. Anat. 132, 61–78 (1971)

    Google Scholar 

  • Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol. 27, 137A (1965)

  • Kikkawa, Y., Hahn, H., Young, S., Bernstein, J.: Mucopolysaccharide in the pulmonary alveolus II. Electron microscopic observations. Lab. Invest. 22, 272–280 (1970)

    Google Scholar 

  • Kikkawa, Y., Manabe, T.: The freeze-fracture study of alveolar type II cells and alveolar content in fetal rabbit lung. Anat. Rec. 190, 627–638 (1978)

    Google Scholar 

  • Kikkawa, Y., Motoyamo, E.K., Gluck, L.: Study of the lungs of fetal and newborn rabbits: Morphological, biochemical and surface physical development. Amer. J. Path. 52, 177–210 (1968)

    Google Scholar 

  • Pease, D.C.: Cytomembrane preservation in tissue dehydrated only with glutaraldehyde and Epon 812 resin. 31st Ann. Proc. Electron Microscopy Soc. Amer., C. J. Arceneaux, ed., pp. 320–321. Baton Rouge, LA: Claitor's Pub. Co. (1973a)

    Google Scholar 

  • Pease, D.C.: Glycol methacrylate copolymerized with glutaraldehyde and urea as an embedment retaining lipids. J. Ultrastruct. Res. 45, 124–148 (1973b)

    Google Scholar 

  • Pease, D.C.: The identification of phospholipid micelles in glutaraldehyde urea embedded tissue. 33rd Ann. Proc. Electron Microscopy, pp. 494–495. Las Vegas, Nevada: Soc. Am. 1975

    Google Scholar 

  • Pease, D.C., Peterson, R.G.: Polymerizable glutaraldehyde-urea mixtures as polar, water containing embedding media. J. Ultrastruct. Res. 41, 133–159 (1972)

    Google Scholar 

  • Reynolds, E.S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208 (1963)

    Google Scholar 

  • Ryan, U.S., Ryan, J.W., Smith, D.S.: Alveolar type II cells: Studies on the mode of release of lamellar bodies. Tissue and Cell 7, 587–599 (1975)

    Google Scholar 

  • Smith, D.S., Smith, U., Ryan, J.W.: Freeze-fractured lamellar body membranes of the rat lung great alveolar cell. Tissue and Cell 4, 457–468 (1972)

    Google Scholar 

  • Sorokin, S.P.: A morphological and cytochemical study on the great alveolar cell. J. Histochem. Cytochem. 14, 884–897 (1967)

    Google Scholar 

  • Stratton, C.J.: Multilamellar body formation in mammalian lung: An ultrastructural study utilizing three lipid-retention procedures. J. Ultrastruct. Res. 52, 309–320 (1975)

    Google Scholar 

  • Stratton, C.J.: The three-dimensional aspect of mammalian lung multilamellar bodies. Tissue and Cell 8, 639–712 (1976a)

    Google Scholar 

  • Stratton, C.J.: The high resolution ultrastructure of the periodicity and archirecture of lipid retained and extracted lung multilamellar body laminations. Tissue and Cell 8, 713–728 (1976b)

    Google Scholar 

  • Stratton, C.J.: The investigation of vinylcyclohexane dioxide as a polar dehydrant for the improved retention of lipids in the Spurr embedment. Tissue and Cell 8, 729–738 (1976c)

    Google Scholar 

  • Stratton, C.J.: The three-dimensional aspect of the mammalian lung surfactant myelin figure. Tissue and Cell 9, 285–300 (1977a)

    Google Scholar 

  • Stratton, C.J.: The periodicity and architecture of lipid retained and extracted lung surfactant and its origin from multilamellar bodies. Tissue and Cell 9, 301–316 (1977b)

    Google Scholar 

  • Stratton, C.J.: The human surfactant system: The step-wise formation of multilamellar bodies, their three-dimensional aspect and morphology, the three-dimensional aspect, morphology, turnover and quantification of surfactant. Abstract of a paper delivered at the Second Annual Conference on Neonatal and Perinatal Medicine, April 22, 1977, Scotssdale, Arizona (1977c)

  • Stratton, C.J.: The surfactant system of human fetal lung in organotypic cultures: Ultrastructural preservation by a lipid-carbohydrate retention method. Anat. Rec. (1978a)

  • Stratton, C.J.: The three-dimensional aspect of lipid and carbohydrate retained, human lung multilamellar bodies. Anat. Rec. 190, 553 (1978b)

    Google Scholar 

  • Sud, B.N.: Electron microscopic study of the human type II pneumonocyte and surfactant synthesis. Anat. Rec. 187, 724–725 (1977)

    Google Scholar 

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Affiliations

  1. Department of Anatomy, University of Nevada-Reno, 89557, Reno, Nevada, USA

    Clifford J. Stratton

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  1. Clifford J. Stratton
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Additional information

Appreciation is expressed to Nuket Olson and Phil Offenhauser for their technical assistance. Supported by a grant from the American Lung Association

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Stratton, C.J. The ultrastructure of multilamellar bodies and surfactant in the human lung. Cell Tissue Res. 193, 219–229 (1978). https://doi.org/10.1007/BF00209036

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  • DOI: https://doi.org/10.1007/BF00209036

Key words

  • Multilamellar bodies (human lung)
  • Surfactant
  • Ultrastructure