Summary
The lack of glutathione S-transferase μ (GSTμ) was examined in 45 healthy French Caucasians and 45 alcoholic cirrhotic French Caucasians: microsamples of blood were taken and DNA amplified by the polymerase chain reaction. We have concluded that there is no relationship between this genotype and the development of alcoholic cirrhosis in these heavy consumers of ethanol.
References
Board PG (1981) Biochemical genetics of glutathione S-transferase in man. Am J Hum Genet 33:36–43
Board PG, Suzuki T, Shaw DC (1988) Human muscle glutathione S-transferase (GST4) shows close homology to human liver GST1. Biochim Biophys Acta 953:214:217
Comstock KE, Sanderson JS, Claflin G, Henner WD (1990) GST1 gene deletion determined by polymerase chain reaction. Nucleic Acids Res 18:3670
Couzigou P, Fleury B, Groppi A, Begueret J, Iron A (1990) Genotypic study of alcohol dehydrogenase class I polymorphism in French alcoholic cirrhosis patient. Alcohol Alcohol 25:623–626
Fleury B, Nalpas B, Couzigou P, Iron A, Poupon RE (1988) Les isoenzymes de l'alcool déshydrogénase et de l'aldéhyde deshydrogénase: rôle dans le métabolisme et al toxicité hépatique de l'alcool. Gastroenterol Clin Biol 12:912–920
Groppi A, Begueret J, Iron A (1990) Improved methods for genotype determination of human alcohol dehydrogenase (ADH) at ADH2 and ADH3 loci by using PCR-directed mutagenesis. Clin Chem 36:1765–1768
Harada S, Abei M, Tanaka N, Agarwal DP, Goedde HW (1987) Liver glutathione S-transferase polymorphism in Japanese and its pharmacogenetic importance. Hum Genet 75:322–325
Hrubec Z, Omenn GS (1981) Evidence of genetic predisposition to alcoholic cirrhosis and psychosis: twin concordances for alcoholism and its biological end points by zygosity among male veterans. Alcoholism (CNY) 5:207–215
Hussey AJ, Hayes JD, Beckett GJ (1987) The polymorphic expression of neutral glutathione S-transferase in human mononuclear leukocytes as measured by specific radio immuno assay. Biochem Pharmacol 36:4013–4015
Laisney V, VanCong N, Gross MS, Frezal J (1984) Human genes for glutathione S-transferases. Hum Genet 68:221–227
Mannervik B (1985a) The isozymes of glutathione S-transferases. Adv Enzymol 57:357–417
Mannervik B, Alin P, Guthenberg C, Jensson H, Tahir MK, Warholm M, Jörnvall H (1985b) Identification of three classes of cytosolic glutathione common to several mammalian species: correlation between structural data and enzymatic properties. Proc Natl Acad Sci USA 82:7202–7206
Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification of β-globin genomic sequences and restriction site analyses for diagnosis of sickle cell anemia. Science 230:1350–1354
Seidegard J, DePierre JW, Pero RV (1985) Hereditary interindividual differences in the glutathione transferase activity towards trans-stilbene oxide in resting human mononuclear leukocytes are due to a particular isozyme(s). Carcinogenesis 6:1211–1216
Seidegard J, Pero RW, Miller DG, Beattie EJ (1986) A glutathione transferase in human leukocytes as a marker for the susceptibility to lung cancer. Carcinogenesis 7:751–753
Seidegard J, Vorachek WR, Pero RW, Pearson WR (1986) Hereditary differences in the expression of the human glutathione tranferase active on trans-stilbene oxide are due to a gene deletion. Proc Natl Acad Sci USA 85:7293–7297
Seidegard J, Pero RW, Markowitz MM, Roush G, Miller DG, Beattie EJ (1990) Isoenzyme(s) of glutathione transferase (class Mu) as a marker for the susceptibility to lung cancer: a follow up study. Carcinogenesis 11:33–36
Sparnins VL, Venegar PL, Wattenberg LW (1982) Glutathione Stransferase activity. Enhancement by compounds inhibiting carcinogenesis and dietary constituents. J Natl Cancer Inst 68:493–496
Strange RC, Faulder CG, Davis BA, Hume R, Brown JAH, Cotton W, Hopkinson DA (1984) The human glutathione S-transerases: studies on the tissue distribution and genetic variation of the GST1, GST2 and GST3 isozymes. Ann Hum Genet 48:11–20
Suzuki T, Coggan M, Shaw DV, Board PG (1987) Electrophoretic and immunological analysis of human glutathione S-transferase isozymes. Ann Hum Genet 51:95–106
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Groppi, A., Coutelle, C., Fleury, B. et al. Glutathione S-transferase class μ in French alcoholic cirrhotic patients. Hum Genet 87, 628–630 (1991). https://doi.org/10.1007/BF00209028
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DOI: https://doi.org/10.1007/BF00209028