European Biophysics Journal

, Volume 23, Issue 2, pp 125–132

Na+, K+ and Cl selectivity of the permeability pathways induced through sterol-containing membrane vesicles by amphotericin B and other polyene antibiotics

  • Scott C. Hartsel
  • Sandra K. Benz
  • Woubeshet Ayenew
  • Jacques Bolard

DOI: 10.1007/BF00208866

Cite this article as:
Hartsel, S.C., Benz, S.K., Ayenew, W. et al. Eur Biophys J (1994) 23: 125. doi:10.1007/BF00208866


Membrane diffusion potentials induced by amphotericin B (AmB), amphotericin B methyl ester (AmE), N-fructosyl AmB (N FruAmB) and vacidin, an aromatic polyene antibiotic, in ergosterol- or cholesterol-containing egg yolk phosphatidylcholine large unilamellar vesicles (LUV), were measured in various media, in order to determine the relative selectivity of Na+, K+, Cl and other ions in these environments. Changes in the membrane potential were followed by fluorescence changes of 3,3′-dipropylthiadicarbocyanine (diS-C3-(5)). Subtle changes in intercationic selectivity were monitored by measuring biionic potentials, using the fluorescent pH sensitive probe pyranine. In all the cases studied, the intereationic selectivity of the permeability pathways induced by the four antibiotics was weak compared to that of specific biological channels, though distinct differences were noted. With AmB the selectivity appeared to be concentration dependent. Above 5 × 10−7 M, the sequence determined for sterol-free small unilamellar vesicles (SUV) and cholesterol-containing SUV and LUV, Na+ > K+ > Rb+ ≥ Cs+ > Li+ (sulfate salts), corresponded closely to Eisenman selectivity sequence number VII. At 5 × 10−7 M and below the selectivity switched from Na+ > K+ to K+ > Na +. In contrast, Li+ was the most permeant ion for AmB channels in the presence of ergosterol. The selectivity between Na+or K+ vs. Cl varied with the antibiotic. It was very strong with vacidin at concentrations below 5 × 10−7 M, smaller with AmB, nil with AmE and N FruAmB. The selectivities observed were antibiotic, concentration and time de pendent, which confirms the existence of different types of channels.

Key words

Amphotericin B Selectivity Vesicles Polyene Membranes 



amphotericin B


amphotericin B methylester


bilayer membranes


3,3′-dipropylthi-acarbocyanine iodide




egg yolk lecithin


carbonyl cyanide p-trifluoro methoxyphenyl-hydrazone


N-(2-hydroxyethylpiperazine)-N′-(2-ethanesulfonic acid)


large unilamellar vesicles


3-(N-morpholino)propanesulfonic acid

N-Fru AmB

N(1-deoxy-D-fructos-1-yl) amphotericin B

Oxonol V1

bis(3-propyl-5-oxoisoazol-4yl)pentamethine oxonol


small unilamellar vesicles


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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Scott C. Hartsel
    • 1
  • Sandra K. Benz
    • 1
  • Woubeshet Ayenew
    • 1
  • Jacques Bolard
    • 2
  1. 1.The Chemistry DepartmentThe University of Wisconsin-Eau ClaireEau ClaireUSA
  2. 2.L.P.C.B. (UA CNRS 198)University Pierre et Marie Curie, 4 Place JussieuParis CedexFrance

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