Abstract
Ultraviolet resonance Raman scattering spectra from aqueous solutions of hypoxanthine and its deuterated species (C8-deuterated, N-deuterated and C8-, N-deuterated derivatives) have been collected and reported in the spectral region between 400 and 1800 cm−1. The laser excitation wavelengths at 281 nm and 257 nm correspond to preresonance and pure resonance conditions, respectively, with the purine strongly allowed π → π* electronic transition: thus the observed experimental Raman features mainly correspond to inplane vibrational modes. The latter were then assigned according to the Wilson GF method by using an empirical harmonic valence force field. Normal mode calculations are based on a non-redundant set of internal coordinates. The calculated vibrational mode wavenumbers and their isotopic shifts upon selective deuterations are in good agreement with the experimental data. The present normal mode analysis rests on the transferability of the guanine and adenine force constants proposed in recent works based on resonance Raman spectroscopy and neutron inelastic scattering data from these major purine bases.
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Correspondence to: M. Ghomi
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Ulicny, J., Ghomi, M., Tomkova, A. et al. Vibrational analysis and molecular force field of hypoxanthine as determined from ultraviolet resonance Raman spectra of native and deuterated species. Eur Biophys J 23, 115–123 (1994). https://doi.org/10.1007/BF00208865
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DOI: https://doi.org/10.1007/BF00208865