Abstract
A hydroxyproline-rich glycoprotein (HRGP) component of the maize cell wall was shown to be present in different organs of the plant by extraction of cell wall proteins and detection by Western blotting and immunocytochemistry. Antibodies raised against the protein or against synthetic peptides designed from the protein sequence immunoprecipitated a proline-rich polypeptide which was synthesized in-vitro from poly(A) + RNA extracted from different tissues of the plant and from the complete in-vitro-transcribed mRNA. A very low amount of the protein was found in immature embryos. In particular, the protein could not be detected in the scutellum either by Western blotting or by immunocytochemistry. In agreement with this finding, HRGP mRNA was barely detected in the scutellum, in contrast to its accumulation in the embryo axis. Our results indicate the existence of a unique cell wall structure in embryonic tissues from maize as well as a tissuespecific component of the control of maize HRGP gene expression, distinct to others already described such as cell division.
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Abbreviations
- HRGP(s):
-
hydroxyproline-richglycoprotein(s)
- DAP:
-
days after pollination
References
Cassab, G.I., Varner, J.E. (1989) Cell wall proteins. Annu. Rev. Plant Physiol. 39, 321–353
De Vries, S.C., Booij, H., Janssens, R., Vogels, R., Saris, L., Lo Schiavo, F., Terzi, M., van Kammen, A. (1988) Carrot somatic embryogenesis depends on the phytohormone-controlled presence of correctly glycosylated extracellular proteins. Genes Dev. 2, 462–476
Eberhard, S., Doubrava, N., Marfà, V., Mohnen, D., Southwick, A., Darvill, A., Albersheim, P. (1989) Pectic cell wall fragments regulate tobacco thin-cell-layer explant morphogenesis. Plant Cell 1, 747–755
Hong, J.C., Nagao, R.T., Key, J.L. (1987) Characterization and sequence analysis of a developmentally regulated putative cell wall protein gene isolated from soybean. J. Biol. Chem. 262, 8367–8376
Hong, J.C., Nagao, R.T., Key, J.L. (1989) Developmentally regulated expression of soybean proline-rich cell wall protein genes. Plant Cell 1, 937–943
Hood, E.E., Shen, Q.X., Varner, J.E. (1988) A developmentally regulated hydroxyproline-rich glycoprotein in maize pericarp cell walls. Plant Physiol. 87, 138–142
Keller, B., Sauer, N., Lamb, C.J. (1988) Glycine-rich cell wall proteins in bean: gene structure and association of the protein with the vascular system. EMBO J. 7, 3625–3633
Kieliszewski, M., Lamport, D.T.A. (1987) Purification and partial characterization of a hydroxyproline-rich glycoprotein in a graminaceous monocot, Zea mays. Plant Physiol. 85, 823–827
Kieliszewski, M., Leykam, J.F., Lamport, D.T.A. (1990) Structure of the threonine-rich extensin from Zea mays. Plant Physiol. 92, 316–326
Knox, J.P., Day, S., Roberts, K. (1989) A set of cell surface glycoproteins forms an early marker of cell position, but not cell type, in the root apical meristem of Daucus carota L. Development 106, 47–56
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685
Lehrach, H., Diamond, D., Wozney, J., Boedtker, H. (1977) RNA molecular weight determination by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry 21, 4743–4751
Logemann, J., Schell, J., Willmitzer, L. (1987) Improved method for the isolation of RNA from plant tissues. Anal. Biochem. 163, 16–20
Ludevid, M.D., Ruiz-Avila, L., Vallès, M.P., Stiefel, V., Torrent, M., Torné, J.M., Puigdomènech, P. (1990) Expression of genes for cell-wall proteins in dividing and wounded tissues of Zea mays L. Planta 180, 524–529
Mazeau, D., Rumeau, D., Esquerré-Tugayé, M.T. (1982) Two different families of hydroxyproline-rich glycoproteins in mellon callus: biochemical and immunochemical studies. Plant Physiol. 86, 540–546
Memelink, J., Hoge, J.H.C., Schilperoort, R.A. (1987) Cytokinin stress changes the developmental regulation of several defenserelated genes in tobacco. EMBO J. 6, 3579–3583
Montoliu, Ll., Rigau, J., Puigdoménech, P. (1989) A tandem of α-tubulin genes preferentially expressed in radicular tissues from Zea mays. Plant Mol. Biol. 14, 1–15
Morrisey, J.H. (1981) Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal. Biochem. 117, 307–310
Murray, D.R. (1984) Axis-cotyledon relationships during reserve mobilization. In: Seed Physiology, vol. 2, pp. 247–280, Murray, D.R., ed. Academic Press, Australia
Noelken M.E., Wisdom, B.J., Hudson, B.G. (1981) Estimation of the size of collagenous polypeptides by sodium dodecyl sulfatepolyacrylamide gel electrophoresis. Anal. Biochem. 110, 131–136
Phillips, G., Chauvet, M., Chaboute, M.E., Ehling, M., Gigot, C. (1986) Genomic organization and nucleotide sequences of two corn histone H4 genes. Gene 42, 225–229
Prat, S., Cortadas, J., Puigdomènech P., Palau, J. (1985) Nucleic acid (cDNA) and amino acid sequences of the maize endosperm protein glutelin-2. Nucleic Acids Res. 13, 1493–1504
Showalter, A.M., Bell, J.N., Cramer, C.L., Bailey, J.A., Varner, J.E., Lamb, C.J. (1985) Accumulation of hydroxyproline-rich glycoprotein mRNAs in response to fungal elicitor and infection. Proc. Natl. Acad. Sci. USA 82, 6551–6555
Stiefel, V., Pérez-Grau, Ll., Albericio F., Giralt E., Ruiz-Avila, L., Ludevid, M.D., Puigdomènech, P. (1988) Molecular cloning of cDNAs encoding for a putative cell wall protein from Zea mays and immunological identification of related polypeptides. Plant Mol. Biol. 11, 483–493
Stiefel, V., Ruiz-Avila, L., Raz, R., Vallés, M.P., Gómez, J., Pagés M., Martínez-Izquierdo, J.A., Ludevid, M.D., Langdale, J.A., Nelson, T., Puigdomènech, P. (1990) Expression of a maize cell wall hydroxyproline-rich glycoprotein gene in early leaf and root vascular differentiation. Plant Cell 2, 785–793
Varner, J.E. Lin, L.-S. (1989) Plant cell wall architecture. Cell 56, 231–239
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The present work was supported by grants from Plan Nacional de Investigation Cientifica y Técnica (grant BI088-0242) and European Communities (grant BAP-374). L.R.-A. is the recipient of a fellowship from the Plan Nacional de Formación de Personal Investigador.
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Ruiz-Avila, L., Ludevid, M.D. & Puigdomènech, P. Differential expression of a hydroxyproline-rich cell-wall protein gene in embryonic tissues of Zea mays L.. Planta 184, 130–136 (1991). https://doi.org/10.1007/BF00208246
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DOI: https://doi.org/10.1007/BF00208246