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Changes of cytoplasmic free Ca2+ in the green alga Mougeotia scalaris as monitored with indo-1, and their effect on the velocity of chloroplast movements

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Abstract

The fluorescent calcium-sensitive dye 1-[2-amino-5-(6-carboxyindol-2-yl)-phenoxy]-2-(2′-amino-5′-methylphenoxy)-ethane-N,N,N′,N′-tetraacetic acid (indo-1) was loaded by a transplasmalemma pH gradient into filamentous cells and protoplasts of Mougeotia scalaris, such that most of the indo-1 fluorescence originated from the cytoplasm. Incubation of M. scalaris filaments in ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA)-buffered media (-log [Ca2+] (=pCa) 8 versus pCa 3) caused a consistent and significant decrease in the cytoplasmic free [Ca2+]. Pulses of the fluorescence excitation light (UV-A 365 nm, 0.7 s) caused an increase in cytoplasmic free [Ca2+] in M. scalaris that was nearly independent of the external [Ca2+] and of chloroplast dislocation by centrifugation. This calcium flux, highest in UV-A light, compared with blue or red light, probably resulted from a release of Ca2+ from intracellular stores. Increased cytoplasmic [Ca2+] may affect the velocity of chloroplast rotation since UV-A-light-mediated chloroplast movement was faster than in blue or red light. Consistently, the calcium ionophore A23187 and the calcium-channel agonist Bay-K8644 both increased the velocity of the red-light-mediated chloroplast rotation. Based on these and other observations, a Ca2+-induced decrease in cytoplasmic viscosity in Mougeotia is presumed to occur.

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Abbreviations

EGTA:

ethylene glycol-bis-(β-aminoethyl ether)N,N,N′,N′-tetraacetic acid

indo-1:

1-[2-amino-5-(6-carboxyindol-2-yl)-phenoxy]-2-(2′-amino-5′-methylphenoxy)-ethane-N,N,N′,N′tetraacetic acid

pCa:

log [Ca2+]

Pfr :

far-red-absorbing form of phytochrome

Pr :

red-absorbing form of phytochrome

xG :

geometric mean

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Authors and Affiliations

  1. Membranund Bewegungsphysiologie, Botanisches Institut 1, Justus-Liebig-Universität, Senckenbergstrasse 17-25, W-6300, Giessen, Germany

    U. Russ, F. Grolig & G. Wagner

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  1. U. Russ
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  2. F. Grolig
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  3. G. Wagner
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Additional information

Dedicated to Professor Wolfgang Haupt on the occasion of his 70th birthday

This paper is part of the Ph.D. thesis of U. Russ at the Justus-Liebig-Universitat Giessen (FRG). Part of this work has been presented at a meeting on “Calcium and intracellular signalling in plants” in Plymouth, UK, Dec. 1990

We are indebted to Dr. G. Seibold and Dipl. Phys. H. Weintraut for their advice on the technique of microspectrofluorometry and for allowing access to the microspectrophotometric facilities in the Strahlenzentrum der Justus-Liebig-Universität, Giessen, FRG. We thank Mrs. A. Quanz for reliable culture of the algae and evaluation of the videotapes. Bay-K8644 was a generous gift of Bayer AG, Wuppertal, FRG. U. russ was supported by a scholarship according to the Hessisches Graduierten Förderungsgesetz. This work was supported by the Deutsche Forschungsgemeinschaft.

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Russ, U., Grolig, F. & Wagner, G. Changes of cytoplasmic free Ca2+ in the green alga Mougeotia scalaris as monitored with indo-1, and their effect on the velocity of chloroplast movements. Planta 184, 105–112 (1991). https://doi.org/10.1007/BF00208243

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