Abstract
Mutagenesis in vitro of the gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase/ oxygenase (EC 4.1.1.39) from Anacystis nidulans was used to generate novel enzymes. Two conserved residues, threonine 4 and lysine 11 in the N-terminus were changed. The substitution of threonine 4 with serine or valine had little effect on the kinetic parameters. The substitution of lysine 11 with leucine, which is non-polar, increased the K m for ribulose-1,5-bisphosphate from 82 to 190 μM but its replacement with glutamine, which has polar properties, had no appreciable effect.
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Abbreviations
- Rubisco:
-
ribulose-1,5-bisphosphate carboxylase/oxygenase
- RuBP:
-
ribulose-1,5-bisphosphate
- LSU:
-
large sub-unit of Rubisco
- SSU:
-
small subunit of Rubisco
References
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We thank Dr. S. Gutteridge (DuPont, Wilmington, USA) for structural information and for his comments on the results described. The technical assistance of Mr. A. Cowland and Mr. I. Major was invaluable.
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Kettleborough, C.A., Phillips, A.L., Keys, A.J. et al. A point mutation in the N-terminus of ribulose-1,5-bisphosphate carboxylase affects ribulose-1,5-bisphosphate binding. Planta 184, 35–39 (1991). https://doi.org/10.1007/BF00208233
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DOI: https://doi.org/10.1007/BF00208233