Summary
Several environments were sampled in a screening procedure to obtain 23 different dextranase-producing fungal strains. The most productive strains were identified as Penicillium purpurogenum and Paecilomyces lilacinus. The culture medium for P. lilacinus strain 6R was optimized, increasing the initial productivity twofold. The enzyme showed optimal activities at pH 5.4 and 65° C, as well as excellent thermal stability at 60° C. An average K m value of 0.26 g/l was found for dextran over a wide range of substrate molecular mass. The enzyme did not show substrate or product inhibition. From HPLC chromatograms, the 6R dextranase was found to readily reduce dextran to low molecular mass oligosaccharides and isomaltose. An integrated kinetic equation is used to describe batch reactions and application dose.
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References
Box GEP, Wilson KB (1951) On the experimental attainment of optimum conditions. J R Stat Soc Ser B 13:1–45
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
Godfrey T, Reichelt J (1983) Industrial enzymology. The application of enzymes in industry. Nature Press, N. York, NY
Hattori A, Ishibashi K, Sadamasa M (1981) The purification of the dextranase of Chaetomium gracile. Agric Biol Chem 45:2409–2416
Koenig DW, Day DF (1988) Production of dextranase by a Lipomyces starkeyi. Biotechnol Lett 10:117–122
Kosaric N, Yu K, Zajic JE (1973) Dextranase production from Penicillium funiculosum. Biotechnol Bioeng 15:729–741
Lee JM, Fox PF (1985) Purification and characterization of Paecilomyces lilacinus dextranase. Enzyme Microb Technol 7:573–577
Lopez-Munguía CA, Monsan P (1980) Dextran synthesis by immobilized dextransucrase. Biochimie 62:323–329
Peterson GL (1977) A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem 83:346
Pelenc V, López-Munguía CA, Remaud M, Remaud M, Biton J, Michel JM, Paul F, Monsan P (1991) Enzymatic synthesis of oligoalternans. Sci Aliments 11:465–476
Pitt JI (1979) The genus Penicillium and its teleomorphic states. Eupenicillium and Talaromyces. Academic Press, London
Preobrazhenskaya ME, Manikova AI (1975) Dextranase of Penicillium purpurogenum. Dokl Acad Nauk SSSR Ser Biol 224:483
Samson RA (1984) Paecilomyces and some allied Hyphomycetes. Studies in mycology, no. 6. Institute of the Royal Netherlands Academy of Sciences and Letters, The Netherlands
Sawai T, Ikagi I, Nawa A (1976) Identification of an isomaltodextranase producing bacterium Arthrobacter globiformis. Agric Biol Chem 40:1249
Smith JE (1985) Mycotoxins hazards in the production of fungal products and by-products. In: Comprehensive Biotechnology. Moo-Young M (ed) Vol. 4 Pergamon Press, Elmsford, NY, p 543
Sun J, Cheng X, Zhang Y, Yan Z, Zhang S (1988) A strain of Paecilomyces lilacinus producing high quality dextranase. Purification and characterization. Ann NY Acad Sci 542:192–194
Von Arx JA (1971) The genera of fungi sporulating in pure culture. Cramer, Lehre
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Galvez-Mariscal, A., Lopez-Munguia, A. Production and characterization of a dextranase from an isolated Paecilomyces lilacinus strain. Appl Microbiol Biotechnol 36, 327–331 (1991). https://doi.org/10.1007/BF00208151
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DOI: https://doi.org/10.1007/BF00208151