Abstract
Phloem sap was collected from white lupin (Lupinus albus L.), cowpea (Vigna unguiculata L.) and castor bean (Ricinus communis L.) and analysed for gibberellins (GAs) using gas chromatography-mass spectrometry (GC-MS). A large number of GAs were found in the phloem exudate of all three species, particularly where the sap was collected from pods (white lupin and cowpea) and in these legumes GAs representing both the early C-13-hydroxylation and non-hydroxylation pathways of biosynthesis were identified. In the sap collected from the vegetative tissues of castor bean the number of GAs identified was fewer than that in the other species, representing mainly the non-hydroxylation pathway. Data from sap collected from the pedicel and stylar ends of pods and by making feeds of radiolabelled GAs to seeds in situ in white lupin indicate that the GAs present in the phloem are derived mainly from the vegetative tissues of the plant. No evidence for metabolism of GAs in the phloem could be found.
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Bowen MR and Hoad GV (1968) Inhibitor content of phloem and xylem sap obtained from willows (Salix viminalis L.) entering dormancy. Planta 81: 64–70
Croker SJ, Hedden P, Lenton JR and Stoddart JL (1990) Comparison of gibberellins in normal and slender barley seedlings. Plant Physiol 94: 194–200
Hall SM and Baker DA (1972) The chemical composition of Ricinus phloem exudate. Planta 106: 131–140
Hedden P (1982) In vitro metabolism of gibberellins. In: Crozier A (ed) Gibberellins Vol. 1, pp. 99–149. Praeger: USA
Hedden P and Croker SJ (1990) GC-MS analysis of gibberellins in plant tissues. In: Kutacek M, Elliott MC and Machackova I (eds) Molecular Aspects of Hormonal Regulation of Plant Development. pp. 19–30. SBP Academic Publishing bv: The Netherlands
Hoad GV (1967) (+)-Abscisn II, (+)-Dormin in phloem exudate of willow. Life Sciences 6: 1113–1118
Hoad GV (1973) Effect of moisture stress on abscisic acid levels in Ricinus communis L., with particular reference to phloem exudate. Planta 113: 367–372
Hoad GV (1978) Effects of water stress on abscisic acid levels in white lupin (Lupinus albus L.) fruits, leaves and phloem exudate. Planta 142: 287–290
Hoad GV and Bowen MR (1968) Evidence for gibberellin-like substances in phloem exudate of higher plants. Planta 82: 22–32
Hoad GV and Gaskin P (1980) Abscisic acid and related compounds in phloem exudate of Yucca flaccida (Haw.) and coconut (Cocos nucifera L.). Planta 150: 347–348
Hoad GV, Hillman SK and Wareing PF (1971) Studies on the movement of indole auxins in willow (Salix viminali L.). Planta 99: 73–88
Kluge M, Reinhard E and Zeigler A (1964) Gibberellinaktivität von Siebröhrensäften. Naturwissenschaften 6: 145–146
Maxwell FG and Painter RH (1962) Auxins in honeydew of Toxoptera graminum, Therioaphis muculata and Macrosiphum pisi and their relation to degree of tolerance in host plants. Ann Entomol Soc Am 30: 229–233
Pate JS, Peoples MB and Atkins CA (1984) Spontaneous phloem bleeding from cryopunctured fruits of ureide-producing plants. Plant Physiol 74: 499–505
Pate JS, Sharkey PJ and Lewis OAM (1974) Phloem bleeding from legume fruits. A technique for study of fruit nutrition. Planta 120: 229–243
Readman JE (dy1983) Plant hormones and fruit development in Lupinus albus L. Ph.D. Thesis, University of Bristol, pp. 353
Weatherley PE, Peel AJ and Hill GP (1959) The physiology of the sieve tube. Preliminary experiments using aphid mouth parts. J Exp Bot 19: 1–16
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Hoad, G.V., Retamales, J.A., Whiteside, R.J. et al. Phloem translocation of gibberellins in three species of higher plants. Plant Growth Regul 13, 85–88 (1993). https://doi.org/10.1007/BF00207596
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DOI: https://doi.org/10.1007/BF00207596