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Optical methods to measure membrane transport processes

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References

  1. Agre, P., Preston, G., Smith, B., Jung, S., Raina, C., Moon, W., Guggino, W., Nielsen, S. 1993. Aquaporin CHIP: the archetypal molecular water channel. Am. J. Physiol. 265:F463-F476

    Google Scholar 

  2. Al-Zahid, B., Schafer, J.A., Troutman, S.L., Andreoli, T.E. 1977. Effect of antidiuretic hormone on water and solute permeation, and the activation energies of these processes in mammalian cortical collecting tubules. J. Membrane Biol. 31:103–129

    Google Scholar 

  3. Andreoli, T.E., Hoffman, J.F., Fanestil, D.D., Schultz, S.G. 1986. Physiology of Membrane Disorders. Plenum Press, New York

    Google Scholar 

  4. Bae, H.-R., Verkman, A.S. 1990. Protein kinase A regulates chloride conductance in endocytic vesicles from proximal tubule. Nature 348:635–637

    Google Scholar 

  5. Barry, P.H., Diamond, J.M. 1984. Effects of unstirred layers on membrane phenomena. Physiol. Rev. 64:763–872

    Google Scholar 

  6. Bicknese, S., Periasamy, N., Shohet, S.B., Verkman, A.S. 1993. Cytoplasmic viscosity near the cell plasma membrane: measurement by evanescent field frequency-domain microfluorimetry. Biophys.J. 65:1272–1282

    Google Scholar 

  7. Bicknese, S., Shahrohk, Z., Shohet, S.B., Verkman, A.S. 1992. Single photon radioluminescence. I. Theory and spectroscopic properties. Biophys. J. 63:1256–1266

    Google Scholar 

  8. Biwersi, J., Farah, N., Wang, Y.-X., Ketchum, R., Verkman, A.S. 1992. Synthesis of cell-impermeable Cl-sensitive fluorescent indicators with improved sensitivity and optical properties. Am. J. Physiol. 262:C243-C250

    Google Scholar 

  9. Biwersi, J., Tulk, B., Verkman, A.S. 1994. Long wavelength chloride-sensitive fluorescent indicators. Anal. Biochem. 219:139–143

    Google Scholar 

  10. Biwersi, J., Verkman, A.S. 1991. Cell permeable fluorescent indicator for cytosolic chloride. Biochemistry 30:7879–7883

    Google Scholar 

  11. Borle, A.B., Bender, C. 1991. Effects of pH on Ca 2+ i , Na + i , and pH i of MDCK cells: Na+-Ca2+ and Na+-H+ antiporter interactions. Am. J. Physiol. 261: C482-C489

    Google Scholar 

  12. Brahm, J. Diffusional water permeability of human erythrocytes and their ghosts. J. Gen. Physiol. 79:791–819

  13. Cabrini, G., Verkman, A.S. 1986. Potential sensitive response mechanism of diS-C3-(5) in biological membranes. J. Membrane Biol. 92:171–182

    Google Scholar 

  14. Chao, A.C., Dix, J.A., Sellers, M., Verkman, A.S. 1989. Fluorescence measurement of chloride transport in monolayer cultured cells: mechanisms of chloride transport in fibroblasts. Biophys. J. 56:1071–1081

    Google Scholar 

  15. Chao, A.C., Widdicombe, J.H., Verkman, A.S. 1990. Chloride transport mechanisms in cultured canine tracheal epithelial cells measured by an entrapped fluorescent indicator. J. Membrane Biol. 113:193–202

    Google Scholar 

  16. Chen, P.−Y., Illsley, N.P., Verkman, A.S. 1988. Renal brush border chloride transport mechanisms characterized using a fluorescent indicator. Am. J. Physiol. 254:F114-F120

    Google Scholar 

  17. Chen, P−Y., Pearce, D., Verkman, A.S. 1988. Membrane water and solute permeability determined quantitatively by self−quenching of an entrapped fluorophore. Biochemistry 27:5713–5719

    Google Scholar 

  18. Chen, P.-Y., Verkman, A.S. 1987. Nonelectrolyte transport across renal proximal tubule cell membranes measured by tracer efflux and light scattering. Pfluegers Arch. 408:491–496

    Google Scholar 

  19. Cheng, S.H., Rich, D.P., Marshall, J., Gregory, R.J., Welsh, M.J., Smith, A.E. 1991. Phosphorylation of the R domain by cAMP-dependent protein kinase regulates the CFTR chloride channel. Cell 66:1027–36

    Google Scholar 

  20. Dho, S., Foskett, J.K. 1993. Optical imaging of Cl-permeabilities in normal and CFTR-expressing mouse L cells. Biochim. Biophys. Acta 1152:83–90

    Google Scholar 

  21. Dunn, S.M.J., Martin, C. Agey, M.W., Miyazaki, R. 1989. Functional reconstitution of the bovine brain GABA receptor from solubilized components. Biochemistry 28:2545–2551

    Google Scholar 

  22. Echevarria, M., Verkman, A.S. 1992. Optical measurement of osmotic water transport in cultured cells: evaluation of the role of glucose transporters. J. Gen. Physiol. 99:573–589

    Google Scholar 

  23. Etter, E.F., Kuhn, M.A., Fay, F.S. 1994. Detection of changes in near-membrane Ca2+ concentration using a novel membrane associated with Ca2+ indicator. J. Biol. Chem. 269:10141–10149

    Google Scholar 

  24. Farinas, J., Simenak, V., Verkman, A.S. 1995. Cell volume measured in adherent cells by total internal reflection microfluorimetry: application to permeability in cells transfected with water channel homologs. Biophys. J. 68:1613–1620

    Google Scholar 

  25. Finkelstein, A. 1987. Water Movement through Lipid Bilayers, Pores, and Plasma Membranes: Theory and Reality. New York, Wiley & Sons

    Google Scholar 

  26. Fischbarg, J., Kunyan, K., Hirsch, J., Lecuona, S., Rogozinski, L., Silverstein, S., Loike, J. 1989. Evidence that the glucose transporter serves as a water channel in J774 macrophages. Proc. Natl. Acad. Sci. USA 86:8397–8401

    Google Scholar 

  27. Fischbarg, J., Kunyan, K., Vera, J.C., Arant, S., Silverstein, S., Loike, J., Rosen, O.M. 1990. Glucose transporters serve as water channels. Proc. Natl. Acad. Sci. USA 87:3244–3247

    Google Scholar 

  28. Flamion, B., Spring, K.R. 1990. Water permeability of apical and basolateral cell membranes of rat inner medullary collecting duct. Am. J. Physiol. 259:F986-F999

    Google Scholar 

  29. Frederickson, C.J., Kasarskis, E.J., Ringo, D., Frederickson, R.E. 1987. A quinoline fluorescence method for visualizing and assaying the histochemically reactive zinc (Bouton zinc) in the brain. J. Neurosci. Meth. 20:91–103

    Google Scholar 

  30. Garcia-Calvo, M., Ruiz-Gomez, A., Vazquez, J., Morato, E., Val-divieso, R., Mayor, F. 1989. Functional reconstitution of the glycine receptor. Biochemistry 28:6405–6409

    Google Scholar 

  31. Giuliano, K., Gillies, R. 1987. Determination of intracellular pH of BALB/c-3T3 cells using fluorescence of pyranine. Anal. Biochem. 167:362–371

    Google Scholar 

  32. Graber, M.L., DiLillo, D.C., Friedman, B.L., Pastoriza, E., Munoz, E. 1986. Characteristics of fluoroprobes for measuring intracellular pH. Anal. Biochem. 156:202–212

    Google Scholar 

  33. Harootunian, A.T., Kao, J.P., Eckert, B.K., Tsien, R.Y. 1989. Fluorescence ratio imaging of cytosolic free Na+ in individual fibroblasts and lymphocytes. J. Biol. Chem. 264:19458–19467

    Google Scholar 

  34. Harris, H. W., Handler, J.S., Blumenthal, R. 1990. Apical membrane vesicles of ADH-stimulated toad bladder are highly water permeable. Am. J. Physiol. 258:F237-F243

    Google Scholar 

  35. Hartmann, T., Verkman, A.S. 1990. Model of ion transport regulation in chloride-secreting airway epithelial cells: integrated description of electrical, chemical and fluorescence measurements. Biophys. J. 58:391–401

    Google Scholar 

  36. Illsley, N.P., Verkman, A.S. 1987. Membrane chloride transport measured using a chloride-sensitive fluorescent indicator. Biochemistry 26:1215–1219

    Google Scholar 

  37. Jiang, C., Finkbeiner, W.E., Widdicombe, J.H., McCray, P.B., Jr., Miller, S.S. Altered fluid transport across airway epithelium in cystic fibrosis. Science 262:424–427

  38. Jung, D.W., Apel, L., Brierley, G.P. 1990. Matrix free Mg2+ changes with metabolic state in isolated heart mitochondria. Biochemistry 29:4121–4128

    Google Scholar 

  39. Kao, H.P., Verkman, A.S. 1994. Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position. Biophys. J. 67:1291–1300

    Google Scholar 

  40. Kedem, O., Katchalsky, A. 1958. Thermodynamic analysis of the permeability of biological membranes to nonelectrolytes. Biochim. Biophys. Acta 27:229–246

    Google Scholar 

  41. Kim, Y., Illsley, N.P., Verkman, A.S. 1988. Rapid fluorescence assay of glucose and neutral solute transport using an entrapped volume indicator. Anal. Biochem. 172:403–409

    Google Scholar 

  42. Krapf, R., Berry, C.A., Verkman, A.S. 1988. Estimation of intracellular chloride activity in isolated perfused rabbit proximal tubules using a fluorescent probe. Biophys. J. 53:955–962

    Google Scholar 

  43. Krapf, R., Illsley, N.P., Tseng, H.C., Verkman, A.S. 1988. Structure-activity relationships of chloride-sensitive fluorescent indicators for biological application. Anal. Biochem. 169:142–150

    Google Scholar 

  44. Kuwahara, M., Berry, C.A., Verkman, A.S. 1988. Rapid development of vasopressin-induced hydroosmosis in kidney collecting tubules measured by a new fluorescence technique. Biophys. J. 54:595–602

    Google Scholar 

  45. Kuwahara, M., Shi, L.-B., Marumo, F., Verkman, A.S. 1991. Transcellular water flow modulates water channel exocytosis and endocytosis in kidney collecting tubule. J. Clin. Invest. 88:423–429

    Google Scholar 

  46. Kuwahara, M., Verkman, A.S. 1988. Direct fluorescence measurement of diffusional water permeability in the vasopressin-sensitive kidney collecting tubule. Biophys. J. 54:587–593

    Google Scholar 

  47. Levitt, D.G., Mlekoday, H.J. 1983. Reflection coefficient and permeability of urea and ethyleneglycol in the human red cell membrane. J. Gen. Physiol. 81:239–254

    Google Scholar 

  48. Loew, L.M. 1992. Voltage-sensitive dyes: measurement of membrane potentials induced by DC and AC electric fields. Bioelectromag. Suppl. 1:179–189

    Google Scholar 

  49. Loew, L.M., Cohen, L.B., Dix, J., Fluhler, E.N., Montana, V., Salama, G., Wu, J.Y. 1992. A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations. J. Membrane Biol. 130:1–10

    Google Scholar 

  50. Lukacs, G.L., Kapus, A., Nanda, A., Romanek, R., Grinstein, S. 1993. Proton conductance of the plasma membrane: properties, regulation, and functional role. Am. J. Physiol. 265: C3-C14

    Google Scholar 

  51. MacVinish, L.J., Reancharoen, T., Cuthbert, A.W. 1993. Kinin-induced chloride permeability changes in colony 29 epithelia estimated from 125I efflux and MEQ fluorescence. Brit. J. Pharm. 108:469–478

    Google Scholar 

  52. Mason, M.J., Grinstein, S. 1990. Effect of cytoplasmic acidification on the membrane potential of T-lymphocytes: role of trace metals. J. Membrane Biol. 116:139–148

    Google Scholar 

  53. Minta, A., Kao, J., Tsien, R. 1989. Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. J. Biol. Chem. 264:8171–8178

    Google Scholar 

  54. Minta, A., Tsien, R.Y. 1989. Fluorescent indicators for cytosolic sodium. J. Biol. Chem. 264:19449–19457

    Google Scholar 

  55. Moore, E.D., Fay, F.S. 1993. Isoproterenol stimulates rapid extrusion of sodium from isolated smooth muscle cells. Proc. Natl. Acad. Sci. USA 90:8058–8062

    Google Scholar 

  56. Muallem, S., Zhang, R., Loessberg, P.A., Star, R.A. 1992. Simultaneous recording of cell volume changes and intracellular pH or Ca2+ concentration in single osteosarcoma cells UMR-106–01. J. Biol. Chem. 267:17658–17664

    Google Scholar 

  57. Mulberg, A.E., Tulk, B.M., Forgac, M. 1991. Modulation of coated vesicle chloride channel activity and acidification by reversible protein kinase A-dependent phosphorylation. J. Biol. Chem. 266:20590–20593

    Google Scholar 

  58. Negulescu, P.A., Machen, T.E. 1990. Intracellular ion activities and membrane transport in parietal cells measured with fluorescent dyes. Meth. Enzymol. 192:38–81

    Google Scholar 

  59. Pearce, D., Verkman, A.S. 1989. NaCl reflection coefficients in proximal tubule apical and basolateral membrane vesicles: measurement by induced osmosis and solvent drag. Biophys. J. 55:1251–1259

    Google Scholar 

  60. Plasek, J., Dale, R.E., Sigler, K., Laskay, G. 1994. Transmembrane potentials in cells: a diS-C3(3) assay for relative potentials as an indicator of real changes. Biochim. Biophys. Acta 1196:181–190

    Google Scholar 

  61. Ram, S.J., Kirk, K.L. 1989. Cl permeability of human sweat duct cells monitored with fluorescence-digital imaging microscopy: evidence for reduced plasma membrane Cl permeability in cystic fibrosis. Proc. Natl. Acad. Sci. USA 24:10166–10170

    Google Scholar 

  62. Reers, M., Smith, T.W., Chen, L.B. 1991. J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential. Biochemistry 30:4480–4486

    Google Scholar 

  63. Seamer, L.C., Mendler, R.N. 1992. Method to improve the sensitivity of flow cytometric membrane potential measurements in mouse spinal cord cells. Cytometry 13:545–552

    Google Scholar 

  64. Seksek, O., Biwersi, J., Verkman, A.S. 1995. Direct measurement of trans-Golgi pH in living cells and regulation by second messengers. J. Biol. Chem. 270:4967–4970

    Google Scholar 

  65. Shahrohk, Z., Bicknese, S., Shohet, S.B., Verkman, A.S. 1992. Single photon radioluminescence. II. Experimental detection and biological applications. Biophys. J. 63:1267–1279

    Google Scholar 

  66. Shi, L.-B., Fushimi, K., Verkman, A.S. 1991. Solvent drag measurement of transcellular and basolateral membrane NaCl reflection coefficient in mammalian proximal tubule. J. Gen. Physiol. 98:379–398

    Google Scholar 

  67. Shi, L.-B., Verkman, A.S. 1989. Very high water permeability in vasopressin-dependent endocytic vesicles in toad urinary bladder. J. Gen. Physiol. 94:1101–1115

    Google Scholar 

  68. Sullivan, L.P., Grantham, J.A., Rome, L., Wallace, D., Grantham, J.J. 1990. Fluorescein transport in isolated proximal tubules in vitro: epifluorometric analysis. Am. J. Physiol. 258:F46-F51

    Google Scholar 

  69. Thomas, J.A., Buchsbaum, R.N., Zimniak, A., Racker, E. 1979. Intracellular pH measurements in Ehrlich ascites tumor cells utilizing spectroscopic probes generated in situ. Biochemistry 18:2210–2218

    CAS  PubMed  Google Scholar 

  70. Van Hoek, A.N., Verkman, A.S. 1992. Functional reconstitution of the isolated erythrocyte water channel CHIP28. J. Biol. Chem. 267:18267–18269

    Google Scholar 

  71. Verkman, A.S. 1987. Mechanism and kinetics of merocyanine 540 binding to phospholipid membranes. Biochemistry 26:4050–4056

    Google Scholar 

  72. Verkman, A.S. 1987. Passive H+/OH permeability in epithelial brush border membranes. J. Bioenerg. Biomembr. 19:481–493

    Google Scholar 

  73. Verkman, A.S. 1989. Mechanisms and regulation of water permeability in renal epithelia. Am. J. Physiol. 257:C837-C850

    Google Scholar 

  74. Verkman, A.S. 1990. Development and biological applications of chloride-sensitive fluorescent indicators. Am. J. Physiol. 259: C375-C388

    Google Scholar 

  75. Verkman, A.S. 1993. Water Channels. In: Molecular Biology Intelligence Series. pp. 1–18. R.G. Landes, Austin, Texas

    Google Scholar 

  76. Verkman, A.S. 1995. Molecular Biophysics of Kidney Water Channels. In: Molecular Biology of the Kidney in Health and Disease. D. Schlondorff and J.V. Bonventre, editors, pp. 459–468. Marcel Dekker, New York

    Google Scholar 

  77. Verkman, A.S., Biwersi, J. 1995. Chloride-sensitive Fluorescent Indicators. In: Methods in Neurosciences, vol. 27 J. Kraicer and S.J. Dixon, editors, pp. 328–339. Academic Press

  78. Verkman, A.S., Chao, A.C., Hartmann, T. 1992. Hormonal regulation of chloride conductance in cultured polar airway cells measured by a fluorescent indicator. Am. J. Physiol. 262: C23-C31

    Google Scholar 

  79. Verkman, A.S., Dix, J.A., Seifter, J.L. 1985. Water and urea transport in renal microvillus membrane vesicles. Am. J. Physiol. 248:F650-F655

    Google Scholar 

  80. Verkman, A.S., Lencer, W., Brown, D., Ausiello, D.A. 1988. Endosomes from kidney collecting tubule contain the vasopressin-sensitive water channel. Nature 333:268–269

    Google Scholar 

  81. Verkman, A.S., Sellers, M., Chao, A.C., Leung, T., Ketcham, R. 1989. Synthesis and characterization of improved chloride-sensitive fluorescent indicators for biological applications. Anal. Biochem. 178:355–361

    Google Scholar 

  82. Verkman, A.S., Takla, R., Sefton, B., Basbaum, C., Widdicombe, J.H. 1989. Quantitative fluorescence measurement of chloride transport in phospholipid vesicles. Biochemistry 28:4240–4244

    Google Scholar 

  83. Whitaker, J.E., Haugland, R.P., Prendergast, F.G. 1991. Spectral and photophysical studies of benzoacxanthene dyes: dual emission of pH sensors. Anal. Biochem. 194:330–344

    Google Scholar 

  84. Wilson, H.A., Seligman, B.E., Chused, T.M. 1985. Voltage-sensitive cyanine dye fluorescence signals in lymphocytes: plasma membrane and mitochondrial components. J. Cell Biol. 125:61–71

    Google Scholar 

  85. Xia, P., Persson, B.-E., Spring, K.R. 1995. The chloride concentration in the lateral intercellular space of MDCK cell monolayers. J. Membrane Biol. 144:21–30

    Google Scholar 

  86. Ye, R., Verkman, A.S. 1989. Osmotic and diffusional water permeability measured simultaneously in cells and liposomes. Biochemistry 28:824–829

    Google Scholar 

  87. Zhang, R., Logee, K., Verkman, A.S. 1990. Expression of mRNA coding for kidney and red cell water channels in Xenopus oocytes. J. Biol. Chem. 265:15375–15378

    Google Scholar 

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I thank Joachim Biwersi, Javier Farinas and Nallagounder Periasamy for critical review of this manuscript. This work was supported by grants DK43840, DK35124, HL42368 and HL85854 from the National Institutes of Health, and grants from the National Cystic Fibrosis Foundation and American Heart Association.

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Verkman, A.S. Optical methods to measure membrane transport processes. J. Membarin Biol. 148, 99–110 (1995). https://doi.org/10.1007/BF00207267

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