Summary
Seventeen gastric carcinomas (intestinal n=12; diffuse n=1; mixed type n=4) and one Barrett's carcinoma were prospectively studied by immunohistochemistry for the expression of different keratin polypeptides and of the brush border markers villin, sucrase isomaltase and aminopeptidase N. All carcinomas expressed the keratin polypeptides 8, 18, and 19 and were stained by the broad specific keratin antibody KL1, irrespective of histologic type. Keratin 7, however, was expressed in only one carcinoma in most tumor cells and in two further carcinomas in some tumor cells. Thus, specific differentiation of the various histologic types of gastric carcinoma does not seem to be aided by the use of keratin antibodies. Villin was positive in 80% of the tumors and sucrase isomaltase and aminopeptidase N were positive in 67% respectively with no obvious histologic difference. The frequent positivity of the brush border markers, usually typical for intestinal epithelium, reflects the high degree of intestinal differentiation of gastric carcinomas, but again does not seem to be associated with a particular histologic type.[/p]
Zusammenfassung
Siebzehn Magenkarzinome (intestinal n=12; diffus n=1; Mischtyp n=4) und ein BarrettKarzinom wurden prospektiv hinsichtlich der Expression verschiedener Keratinpolypeptide sowie der Bürsten-saummarker Villin, Sucrase Isomaltase and Aminopeptidase N immunfluoreszenzmikroskopisch untersucht. Unabhängig vom histologischen Typ exprimierten alle Karzinome die Keratinpolypeptide 8, 18 and 19 and reagierten mit dem breit spezifischen Keratinantikbrper KL1. Das Keratin 7 hingegen wurde nur in einem Karzinom von nahezu allen Tumorzellen and bei zwei weiteren Karzinomen nur von einigen Tumorzellen exprimiert. Die weitergehende Differenzierung der verschiedenen hi stologischen Typen des Magenkarzinoms ist mit Hilfe der Keratinantikörper nicht m6glich. Villin war in 80% aller Karzinome and Sucrase Isomaltase and Aminopeptidase N waren in je 67%, wiederum ohne histologiespezifische Unterschiede, positiv. Die Nachweisbarkeit der Bürstensaummarker, charakteristisches Kennzeichen des intestinalen Epithels, verdeutlicht das hohe Maß der intestinalen Differenzierung der Magenkarzinome, ohne daß eine Zuordnung zu bestimmten histologischen Typen möglich erscheint.[/p]
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Jähne, J., Osborne, M., Meyer, H.J. et al. Ermöglichen immunologische Marker eine Differenzierung zwischen den histologischen Typen des Magenkarzinoms?. Langenbecks Arch Chir 375, 135–140 (1990). https://doi.org/10.1007/BF00206805
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DOI: https://doi.org/10.1007/BF00206805