, Volume 195, Issue 1, pp 150–152 | Cite as

Limited period of graviresponsiveness in germinating spores of Ceratopteris richardii

  • Erin S. Edwards
  • Stanley J. RouxEmail author
Rapid Communication


Rhizoids of the fern Ceratopteris richardii Brogn. usually emerge 40 h after germination is initiated by light, and more than 90% of them emerge growing in a downward direction. However, when the spores are germinated on a clinostat, the emerging rhizoids show no preferential orientation. This indicates that under normal 1 · g conditions the initial growth direction of rhizoids can be oriented by gravity. If the orientation of the spores is changed 3 h or less after the start of germination, the growth direction of most emerging rhizoids becomes downward relative to the new orientation. However, if the orientation of the spores is changed by 180° 8 h or more after germination is initiated by light, most rhizoids emerge growing upward; i.e., the same direction as if there had been no orientation change. Emerged rhizoids also do not change their direction of growth if their orientation is changed. These results indicate that the growth direction of emerging rhizoids is set by gravity prior to actual emergence, and that the time of full orientation responsiveness is limited to a period ranging from the initiation of germination to about 3–4 h after the start of germination. There is a gravity-oriented nuclear movement beginning at about 13 h after germination, and this movement appears to predict the initial growth direction of rhizoids.

Key words

Cell polarity Ceratopteris Fern gametophyte Gravity Nuclear migration Rhizoid 


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  1. Bloom, W.W., Nichols, K.E. (1972) Rhizoid formation in megagametophytes of Marsilea in response to growth substances. Am. Fern J. 62, 24–26Google Scholar
  2. Chasan, R. (1992) Ceratopteris: a model plant for the 90s. Plant Cell 4, 113–115Google Scholar
  3. Cooke, T.J., Racusen, R.H., Hickok, L.G., Warne, T.R. (1987) The photocontrol of spore germination of the fern Ceratopteris richardii. Plant Cell Physiol. 28, 753–759Google Scholar
  4. Hickok, L.G., Warne, T.R., Slocum, M.K. (1987) Ceratopteris richardii: applications for experimental plant biology. Am. J. Bot. 74, 1304–1316Google Scholar
  5. Warne, T.R., Walker, G.L., Hickok, L.G. (1986) A novel method for surface-sterilizing and sowing fern spores. Am. Fern J. 76, 187–188Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  1. 1.Department of BotanyThe University of Texas at AustinAustinUSA
  2. 2.Department of Plant PhysiologyWageningen Agricultural UniversityWageningenThe Netherlands

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