Summary
Panels of 3 M KCl extracts of squamous-cell carcinomas, adenocarcinomas and oat-cell carcinomas of the lung were used for a comprehensive analysis of cross-reactivity in the leucocyte migration test. Lung cancer patients' leucocytes showed positive reactivity in 69%–100% of cases (n=353). No significant differences were observed when data were grouped with respect to the histological type of the tumours used for extraction or of the tumours of the leukocyte donors. Leukocytes of patients bearing tumours of nonpulmonary origin exposed to lung cancer extract panels and leukocytes of lung cancer patients exposed to gastrointestinal cancer extract panels were definitely less reactive (35%–47% and 6%–38%, respectively). However, a high reaction frequency was found in patients with lung metastases from different nonpulmonary tumours. This group of patients also frequently showed reactivity (52%) with normal lung tissue extracts. Patients with benign lung diseases reacted positively with lung tumour extracts in 25%–39% of cases, but donors with other benign disease and healthy controls were virtually nonreactive (0–14%).
Hence, a high degree of cross-reactivity occurs in the lung cancer system and restricted cross-reactivity occurs with tumours of other organs. Possible explanations for the lung-oriented reactivity of patients with lung metastases are discussed.
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Abbreviations
- LMI:
-
leucocyte migration inhibition
- MI:
-
migration index
- LMT:
-
leucocyte migration test
- SCC:
-
squamous-cell carcinoma
- OCC:
-
oat-cell carcinoma
- AC:
-
adenocarcinoma
References
Boddie AW Jr, Holmes EC, Roth JA, Morton DL (1975a) Inhibition of human leukocyte migration in agarose by KCl extracts of carcinoma of the lung. Int J Cancer 15:823–829
Cannon GB, McCoy JL, Connor RJ, Jerome L, Keys L, Dean JH, Herberman RB (1978) Use of leukocyte migration inhibition assay to evaluate antigenic differences in human breast cancers and melanomas. J Natl Cancer Inst 60:969–978
Cochran AJ, Grant RM, Spilg WG, Mackie RM, Ross CE, Hoyle DE, Russel JM (1974) Sensitization to tumour-associated antigens in human breast carcinoma. Int J Cancer 14:19–25
Elias EG, Elias LL (1975) Autogenous leukocyte migration in human malignancies. Cancer 36:1393–1398
Federlin K, Maini RN, Russell AS, Dumonde DC (1971) A micromethod for peripheral leukocyte migration in tuberculin sensitivity. J Clin Pathol 24:533–536
Fogel M, Gorelik E, Segal S, Cohen IR, Feldman M (1977) Demonstration of antigenic differences between a local tumor of Lewis lung carcinoma (3LL) and its pulmonary metastases. Isr. J. Med. Sci. 13:1032
Kjaer M, Christensen N (1977) Ability of renal carcinoma tissue extract to induce leukocyte migration inhibition in patients with nonmetastatic renal carcinoma: Correlation with clinical and histopathological findings. Cancer Immunol Immunother 2:41–48
Lurie BB, Bull DM, Zamcheck N, Steward AM, Helms RA (1975) Diagnosis and prognosis in colon cancer based on a profile of immune reactivity. J Natl Cancer Inst 54:319–325
Matzku S, Zöller M, Schulz U (1978) CEA and leukocyte migration test (LMT): Comparison of diagnostic validity and evaluation of a possible involvement of CEA in leukocytes' sensitization. In: Lehmann FG (ed) Carcinoembryonic proteins, vol II. Elsevier/North Holland, Amsterdam, p 819–824
McCoy JL, Jerome LF, Cannon GB, Weese JL, Herberman RB (1977) Reactivity of lung cancer patients in leukocyte migration inhibition assays to 3 M potassium chloride extracts of fresh tumor and tissue-cultered cells derived from lung cancer. J Natl Cancer Inst 59:1413–1418
Matthews MJ (1976) Problems in morphology and behaviour of bronchopulmonary malignant disease. In: Israel L, Chahinian AP (eds) Lung cancer: Natural history, prognosis and therapy. Academic, New York, p 23–62
Sugarbaker EV, Cohen AM (1972) Altered antigenicity in spontaneous pulmonary metastases from an antigenic murine sarcoma. Surgery 72:155–161
Zöller M, Matzku S, Schulz U (1977a) Leukocyte migration studies in gastric cancer detection: an approach towards improved specificity and sensitivity. J Natl Cancer Inst 58:897–904
Zöller M, Matzku S, Schulz U (1977b) Colorectal cancer diagnosis by a direct leukocyte migration test using a panel of tumor extracts. Cancer Immunol Immunother 2:257–265
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Zöller, M., Matzku, S. & Zeidler, D. Cross-reactivity of lung cancer patients' leucocytes in the leucocyte migration inhibition test. Cancer Immunol Immunother 8, 143–148 (1980). https://doi.org/10.1007/BF00205664
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DOI: https://doi.org/10.1007/BF00205664