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The antineoplastic effect of Corynebacterium parvum modified by lysozyme treatment

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Summary

The study was conducted to evaluate the possibility that host factors may modulate the antitumor effect of C. parvum (CP). Heat-killed CP after being subjected to limited digestion by lysozyme (L-CP) produced a superior effect on the inhibition of local growth of murine mammary carcinoma (CD 8 F 1 ) comparsed to unmodified CP. The median survival time (MST) of tumor-bearing animals that were treated with L-CP was slightly but not significantly increased over that of the immunotherapeutic response to unmodified CP. This modified CP was capable of reducing the incidence of early death in tumor-bearing animals. The modified CP was also more toxic than the unmodified. In contrast, mixed glycosidase had no effect on the antitumor potency of CP. The usefulness of enzymatic modification of whole CP in improving the tumor-therapeutic potency of CP is discussed.

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References

  1. Adlam C, Reid DE, Torkington P (1975) The nature of the active principle of C. parvum. In: Halpern B (ed) Corynebacterium parvum and its application in experimental and clinical oncology. Plenum Press, New York, p 33

    Google Scholar 

  2. Curries G (1976) Immunological aspects of host resistance to the development and growth of cancer. Biochim Biophys Acta 458:135

    Google Scholar 

  3. Dawes J, Tuach SJ, McBride WH (1974) Properties of an antigenic polysacharide from Corynebacterium parvum. J Bacteriol 120:24

    Google Scholar 

  4. Gordon S, Todd J, Cohn AZ (1974) In vitro synthesis and secretion of lysozyme of mononuclear phagocytes. J Exp Med 139:1228

    Google Scholar 

  5. Halpern BN, Biozzi G, Stiffel C, Mouton D (1966) Inhibition of tumor growth by administration of killed Corynebacterium parvum. Nature 212:853

    Google Scholar 

  6. Israel L, Edelstein R (1977) Personal experience with Corynebacterium parvum in human cancers. World J Surg 585

  7. Jolles P (1976) A possible physiological function of lysozyme. Biomedicine 25:275

    Google Scholar 

  8. Jolles P, Migliore Samour D, Korontzis M, Folch F, Maral R, Werner GH (1979) Study on soluble substances extracted from Corynebacterium parvum. In: Halpern B (ed) Corynebacterium parvum, and its application in experimental and clinical oncology. Plenum Press, New York, p 40–47

    Google Scholar 

  9. Kouznetzova B, Bizzini B, Cherman JC, Degrand F, Prevót AR, Raynand M (1974) Immunostimulating activity of whole cells, cell walls, and fractions of anaerobic Corynebacteria. Recent Results Cancer Res 47:275

    Google Scholar 

  10. Lanzerotti RH, Gullino PM (1972) Activities and quantities of lysosomal enzymes during mammary tumor regression. Cancer Res 32:2579

    Google Scholar 

  11. Martin DS, Fugmann RA, Stolfi RL, Hayworth PE (1975) Solid tumor animal model therapeutically predictive for human breast cancer. Cancer Chemother Rep. [2] 5:89

  12. McBride WH, Dawes J, Tuach S (1976) Antitumor activity of Corynebacterium parvum extracts. J Natl Cancer Inst 56:437

    Google Scholar 

  13. McClelland DBL, Van Furth R (1975) In vitro synthesis of lysozyme by human and mouse tissue and leukocytes. Immunology 28:1099

    Google Scholar 

  14. Milas L, Scott MT (1978) Antitumor effect of Corynebacterium parvum. Adv Cancer Res 26:257

    Google Scholar 

  15. Milas L, Mason K, Withers HR (1976) Therapy of spontaneous pulmonary metastases of a murine mammary carcinoma with anaerobic Corynebacteria. Cancer Immunol Immunother 1:233

    Google Scholar 

  16. Millman I, Scott AW, Halbherr T (1977) Antitumor activity of Propionibacterium acnes (Corynebacterium parvum) and isolated cytoplasmic fractions. Cancer Res 37:4150

    Google Scholar 

  17. Osserman EF, Klockars M, Halper J, Fischel RE (1973) Effects of lysozyme on normal and transformed mammalian cell. Nature 243:331

    Google Scholar 

  18. Purnell DM, Otterstrom JR, Bartlett GL, Kreider J (1976) Antitumor activity of killed Corynebacterium parvum suspensions in a murine mammary adenocarcinoma (CaD2) system. J Natl Cancer Inst 56:1171

    Google Scholar 

  19. Ribi E, McLaughlin CA, Cantrell JL, Brenmer W, Azuma I, Yamamura Y, Strain SM, Hwang KM. Imunotherapy with compound derived from microorganisms and with synthetic analogs of some of these. A review. In: Immunotherapy of human cancer, Twenty-second Annual Clinical Conference on Cancer. Raven press, New York, p 131

  20. Riveros Moreno V, Bomford R, Scott MT (1978) Antitumor activity of purified cell walls from Corynebacterium parvum. J Natl Cancer Inst 60:653

    Google Scholar 

  21. Russell RJ, McInroy RJ, Wilkinson PC, White RG (1976) A lipid chemotactic factor from anaerobic coryneform bacteria including Corynebacterium parvum with activity of macrophages and monocytes. Immunology 30:935

    Google Scholar 

  22. Shamberger RJ (1969) Lysosomal enzyme changes in growing and regressing mammary tumors. Biochem J 3:375

    Google Scholar 

  23. Stolfi RL, Martin DS, Fugmann RA (1971) Spontaneous murine mammary adenocarcinoma: Model system for evaluation of combined method of therapy, Cancer Chemother Rep [1] 55:239

  24. Stolfi RL, Stolfi LM, Spiegelman S, Martin DS (1978) Systemic treatment of spontaneous autochthonous murine breast tumor (CD8F1) with high-doses C. parvum. Proc Am Assoc Cancer Res 19:915

    Google Scholar 

  25. Terry WD, Windhorst D (ed) (1978) Immunotherapy of cancer: Present status of trials in man. In: Progress in cancer research and therapy, vol. 6. Raven Press, New York

    Google Scholar 

  26. University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute (1978) Immunotherapy of human cancer Twenty-second Annual Clinical Conference on Cancer. Raven Press, New York

    Google Scholar 

  27. Woodruff MFA, Boak JL (1966) Inhibitory effect of injection of Corynebacterium parvum on the growth of tumor transplants in isogenic hosts. Br J Cancer 20:345

    Google Scholar 

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Author notes

  1. Kou M. Hwang

    Present address: LAC/USC Cancer Center, 1303 N. Mission Road, 90033, Los Angeles, California, USA

Authors and Affiliations

  1. Section of Immunology, Department of Developmental Therapeutics MD, Anderson Hospital and Tumor Institute, 77030, Houston, Texas, USA

    Kou M. Hwang, E. M. Hersh, Byung H. Chung, D. S. Martin, R. L. Stolfi & Jordan U. Gutterman

  2. The Catholic Medical Center of Brooklyn and Queens Inc., New York, NY, USA

    D. S. Martin & R. L. Stolfi

Authors
  1. Kou M. Hwang
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  2. E. M. Hersh
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  3. Byung H. Chung
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  4. D. S. Martin
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  5. R. L. Stolfi
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  6. Jordan U. Gutterman
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Hwang, K.M., Hersh, E.M., Chung, B.H. et al. The antineoplastic effect of Corynebacterium parvum modified by lysozyme treatment. Cancer Immunol Immunother 8, 113–117 (1980). https://doi.org/10.1007/BF00205660

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  • DOI: https://doi.org/10.1007/BF00205660

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