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Construction and characterisation of a hybrid-hybrid monoclonal antibody recognising both carcinoembryonic antigen (CEA) and vinca alkaloids

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Summary

Recent developments of hybridoma technology have allowed us to prepare a bispecific monoclonal antibody recognising both the tumour-associated antigen carcinoembryonic antigen (CEA) and the cytostatic vinca alkaloids. The yields of the hybrid-hybrid 28.19.8 monclonal after affinity chromatography purification are close to 50% of the total Ig produced.

The hybrid-hybrid has a molecular weight ca. 150000 daltons. The heavy chains of the hybrid-hybrid are a γ1 heavy chain from the parental anti-CEA monoclonal and a γ2a heavy chain from the anti-vinca alkaloid donor lymphocytes.

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Authors and Affiliations

  1. Lilly Research Centre Ltd, Eli Lilly & Co., Erl Wood Manor, GU20 6PH, Windlesham, Surrey, UK

    J. R. F. Corvalan & W. Smith

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  1. J. R. F. Corvalan
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  2. W. Smith
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Corvalan, J.R.F., Smith, W. Construction and characterisation of a hybrid-hybrid monoclonal antibody recognising both carcinoembryonic antigen (CEA) and vinca alkaloids. Cancer Immunol Immunother 24, 127–132 (1987). https://doi.org/10.1007/BF00205589

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  • DOI: https://doi.org/10.1007/BF00205589

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